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Jun 28, 2023Open Access
We performed aurora kinase gene expression analysis, in combination with FISH and micronucleus test, in buccal mucosa cells isolated from agricultural workers to better understand the dynamics of genomic instability in these groups of professionals. Two out of a total of 97 agricultural workers who consented to participate in the study (39 men and 58 women) reported a history of cancer based on a previously applied questionnaire. The participants were 28.4 years on average and were categorized i...
May 15, 2020Open Access
Chloroquine (CQ) was initially synthesized as an antimalarial agent, but later on, it also shows immunomodulatory, anticancer, and antiviral effects in clinical practice. Although CQ has been used to treat various conditions for more than half century, the underlying mechanisms of its diverse therapeutic actions remain incomplete. In this paper, we hypothesize that targeting three-dimensional genome architecture might be one of the mechanisms of CQ’s diverse therapeutic actions. Based on this hy...
Jun 28, 2019Open Access
We once proposed that cell-type-associated chromatin configurations determine cell types and that cancer cell type is determined by cancer-associated chromatin configuration (CACC). In this paper, we hypothesize that flexible cell-type-associated chromatin configuration is associated with cell potency and has an advantage over inflexible one in regulating genome related activities, such as DNA replication, DNA tra ...
Feb 28, 2019Open Access
More than 30 years ago, we published a hypothesis in which the 3-dimen- sional structure of entire chromatin fibres in a eukaryotic cell is named as chromatin configuration that determines the pattern of gene expression in each type of cell. Based on this hypothesis, cell differentiation is the process in which different types of cells are endowed with different chromatin configura-tions. Therefore, this chrom ...
Feb 01, 2019Open Access
More than 30 years ago, we published a paper entitled as “Abnormal Chromatin Configuration and Oncogenesis”, which proposed the first hy-pothesis that links oncogenesis to abnormal three-dimensional (3D) genome structure. Recently, many studies have demonstrated that the 3D genome structure plays a major role in oncogenesis, which strongly supports our hypothesis. In this paper, further thoughts about our hypothesis are presented. ...
Feb 09, 2017Open Access
Although it is not widely-known,
the strands of circular duplex plasmid and viral chromosomes have been
non-destructively separated, and the separated strands have been reconstituted
to yield a new duplex structure with all the properties of the native chromosome
restored. This suggests a paranemic structure for the DNA, that is, a
structure whose strands are no ...
Jul 26, 2016Open Access
Targeted mutagenesis by
zinc-finger nucleases (ZFNs) can be used to generate knock-out mammalian cell
lines with high efficiency. A number of different methods have been developed
for the design and assembly of gene-specific ZFNs, making them easily accessible
to researchers. In this study, we used ZFNs assembled through the CoDA
(context-dependent assembly) platforms to generate mutant caprine fetal fibroblasts
cells for the BLG ...
Sep 10, 2014Open Access
The major cause of poor
livestock productivity in tropical regions of the world is inadequate nutrition.
The shortage of feed in most developing countries and the increasing cost of
feed constituents entail that there is a call for to improvement feed
utilization strategies in developing countries. Animal feeds and feeding
practices can be enhanced by biotechnology to develop better animal nutrition
packages in smallholder and commercial production systems as well as to reduce
environmental wast...
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