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- 2019
Novel fluorescent probes of 10-hydroxyevodiamine: autophagy and apoptosis-inducing anticancer mechanismsDOI: 10.1016/j.apsb.2018.08.003 Keywords: Boc, di-tert-butyl dicarbonate, CCK8, cell counting kit-8, DMAP, 4-dimethylaminopyridine, DMSO, dimethylsulfoxide, EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, HBTU, O-benzotriazole-N,N,N,N-tetramethyl-uronium-hexafluorophosphate, 3MA, 3-methyladenine, MMP, mitochondrial membrane potential, NPs, natural products, TEA, trimethylamine, TFA, trifluoroacetic acid Fluorescent probes, 10-Hydroxyevodiamine, Anticancer mechanisms, Autophagy, Apoptosis Abstract: Natural product evodiamine and its derivatives represent a promising class of multi-target antitumor agents. However, the clinical development of these compounds has been hampered by a poor understanding of their antitumor mechanisms. To tackle this obstacle, herein, novel fluorescent probes were designed to elucidate the antitumor mode of action of 10-hydroxyevodiamine. This compound was proven to be distributed in the mitochondria and lysosomes and to act by autophagy and apoptosis mechanisms
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