Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried a？aí (Euterpe oleraceae Mart.) by HPLC

availability of analytical standards is a critical aspect in developing methods for quantitative analysis of anthocyanins. the anthocyanins cyanidin-3-o-glucoside and cyanidin-3-o-rutinoside were isolated from samples of freeze-dried a？aí (euterpe oleraceae mart.), which is a round and purple well-known palm fruit in brazil, and then used as standards. the isolation of the anthocyanins was performed by high performance liquid chromatography (hplc), using an adapted six-channel selection valve. the identification of anthocyanin pigments in a？aí was based on mass spectrometric data for molecular ions and ms-ms product ions and on previous published data. after the collection procedure, standards with a high purity grade were obtained and an external standard curve of each anthocyanin was plotted.