lincRNA-Cox2 regulates NLRP3 inflammasome and autophagy mediated neuroinflammation

LincRNA-Cox2 regulates autophagy in an ATG5-dependent manner. a Western blot of LC3-I and LC3-II in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4？h and 1？mM ATP for 30？min, further treated in the presence or absence of 3-MA and CQ. b Ratio of LC3-II/LC3-I in three independent experiments from (a). c Representative immunofluorescence images of LC3 in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4？h and 1？mM ATP for 30？min. LC3 staining is shown in green, and nuclei are blue. Scale bar indicates 10？μm (three independent experiments). d Number of LC3 puncta in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4？h and 1？mM ATP for 30？min. e Effects of Atg5 knockdown on LC3 II levels in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4？h and 1？mM ATP for 30？min. f Ratio of LC3-II/LC3-I in three independent experiments as in (e). g, h Western blot and the ratio of LC3-I and LC3-II in control or lincRNA-Cox2 knockdown BMDM and primary microglia cells treated with LPS for 4？h and 1？mM ATP for 30？min. GAPDH is shown as the loading control in all western blot figures (western blot repeated in three independent experiments). Columns are the mean values of triplicates; the error bar indicates the SEM. The symbol ** indicates a statistically significant difference from the control; p？<？0.0