%0 Journal Article
%T lincRNA-Cox2 regulates NLRP3 inflammasome and autophagy mediated neuroinflammation
%A Hongkun Liu
%A Hongwei Yang
%A Junwei Hao
%A Lijuan Zhang
%A Long Jia
%A Qi Zhang
%A Rongxin Zhang
%A Wen Li
%A Xiangdong Guo
%A Yan Li
%A Ying Liu
%A Yinghui Ren
%A Yurong Da
%A Zhenyi Xue
%A Zhi Yao
%A Zhihui Zhang
%A Zimu Zhang
%J Archive of "Cell Death and Differentiation".
%D 2019
%R 10.1038/s41418-018-0105-8
%X LincRNA-Cox2 regulates autophagy in an ATG5-dependent manner. a Western blot of LC3-I and LC3-II in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4£¿h and 1£¿mM ATP for 30£¿min, further treated in the presence or absence of 3-MA and CQ. b Ratio of LC3-II/LC3-I in three independent experiments from (a). c Representative immunofluorescence images of LC3 in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4£¿h and 1£¿mM ATP for 30£¿min. LC3 staining is shown in green, and nuclei are blue. Scale bar indicates 10£¿¦Ìm (three independent experiments). d Number of LC3 puncta in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4£¿h and 1£¿mM ATP for 30£¿min. e Effects of Atg5 knockdown on LC3 II levels in control or lincRNA-Cox2 knockdown BV2 cells treated with LPS for 4£¿h and 1£¿mM ATP for 30£¿min. f Ratio of LC3-II/LC3-I in three independent experiments as in (e). g, h Western blot and the ratio of LC3-I and LC3-II in control or lincRNA-Cox2 knockdown BMDM and primary microglia cells treated with LPS for 4£¿h and 1£¿mM ATP for 30£¿min. GAPDH is shown as the loading control in all western blot figures (western blot repeated in three independent experiments). Columns are the mean values of triplicates; the error bar indicates the SEM. The symbol ** indicates a statistically significant difference from the control; p£¿<£¿0.0
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294802/