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辣根过氧化物酶的纯度与降解苯甲醛效果关系的研究
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Abstract:
本文研究不同纯度的辣根过氧化物酶(horseradish peroxidase, HRP)催化降解苯甲醛的效果。实验室制备商业级产品A (RZ > 0.8，酶活 > 80 U/mg)，B (RZ > 1.5，酶活 > 150 U/mg)，C (RZ > 3.0，酶活 > 300 U/mg)三种不同纯度的辣根过氧化物酶。通过改变时间、温度、pH、反应物浓度和酶量等变量探究产品B催化转化苯甲醛的最佳反应条件。利用获得的最佳反应条件，确认产品A和C催化降解苯甲醛的效果，发现产品B与A有明显差别，与C没有显著性差别。考虑到不同纯度HRP产品存在显著的价格差异，本研究认为，产品B (RZ > 1.5，酶活 > 150 U/mg)对于降解苯甲醛而言具有最佳经济适用性。
In this paper, the effects of horseradish peroxidase (HRP) with different purities on the catalytic degradation of benzaldehyde were evaluated. Products A (RZ > 0.8, enzyme activity > 80 U/mg), B (RZ > 1.5, enzyme activity > 150 U/mg) and C (RZ > 3.0, enzyme activity > 300 U/mg) were prepared in our lab. The parameters including time, temperature, pH, reactant concentration and enzyme amount were optimized. Using the optimal parameters, the effects of products A and C were evaluated. The results indicated that products B was significantly different from A but similar with product C. Considering the economic issue, these results indicated that Product B (RZ > 1.5, enzyme activity> 150 U/mg) might be the best choice to degrade benzaldehyde for industry.
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