%0 Journal Article
%T 辣根过氧化物酶的纯度与降解苯甲醛效果关系的研究<br>Study on the Relationship between the Purity of Horseradish Peroxidase and Efficacy on the Degradation of Benzaldehyde
%A 严筱文
%A 陈胜胜
%A RajendranSatheeshkumar
%A 刘向晖
%A 王文龙
%J Hans Journal of Medicinal Chemistry
%P 14-20
%@ 2331-8295
%D 2020
%I Hans Publishing
%R 10.12677/HJMCe.2020.82003
%X 本文研究不同纯度的辣根过氧化物酶(horseradish peroxidase, HRP)催化降解苯甲醛的效果。实验室制备商业级产品A (RZ &gt; 0.8，酶活 &gt; 80 U/mg)，B (RZ &gt; 1.5，酶活 &gt; 150 U/mg)，C (RZ &gt; 3.0，酶活 &gt; 300 U/mg)三种不同纯度的辣根过氧化物酶。通过改变时间、温度、pH、反应物浓度和酶量等变量探究产品B催化转化苯甲醛的最佳反应条件。利用获得的最佳反应条件，确认产品A和C催化降解苯甲醛的效果，发现产品B与A有明显差别，与C没有显著性差别。考虑到不同纯度HRP产品存在显著的价格差异，本研究认为，产品B (RZ &gt; 1.5，酶活 &gt; 150 U/mg)对于降解苯甲醛而言具有最佳经济适用性。<br />
In this paper, the effects of horseradish peroxidase (HRP) with different purities on the catalytic degradation of benzaldehyde were evaluated. Products A (RZ &gt; 0.8, enzyme activity &gt; 80 U/mg), B (RZ &gt; 1.5, enzyme activity &gt; 150 U/mg) and C (RZ &gt; 3.0, enzyme activity &gt; 300 U/mg) were prepared in our lab. The parameters including time, temperature, pH, reactant concentration and enzyme amount were optimized. Using the optimal parameters, the effects of products A and C were evaluated. The results indicated that products B was significantly different from A but similar with product C. Considering the economic issue, these results indicated that Product B (RZ &gt; 1.5, enzyme activity&gt; 150 U/mg) might be the best choice to degrade benzaldehyde for industry.
%K 辣根过氧化物酶，苯甲醛，催化氧化，经济适用性<br>Horseradish Peroxidase
%K Benzaldehyde
%K Catalytic Oxidation
%K Economic Applicability
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=34942