In this study, 60 direct seeding rice varieties with different genotypes were used as experimental materials. Through long-term submergence treatment (7 d and 14 d), the responses of different genotypes to submergence stress were compared in germination and seedling emergence, stem, leaf and root elongation, starch and other storage material consumption. The results showed that there were significant differences in submergence tolerance of different genotypes at the germination and seedling stage. Through further analysis by statistical methods such as correlation analysis, principal component analysis, weight comprehensive evaluation and cluster analysis, 60 varieties could be divided into four categories: strong submergence tolerance, medium submergence tolerance, weak submergence tolerance and submergence sensitivity, while 6, 22, 4 and 28 varieties were screened respectively. After 14 d and 4 cm deep submergence, more than 78.1% of rice seeds could germinate, and the average plant height and root length could reach more than 11.99 cm and 9.66 cm respectively. The dry matter mass, starch content and soluble sugar content per plant were significantly higher than those of other tolerant types. The average coleoptile of the sensitive type was only 3.17 cm, and the radicle had little elongation. The evaluation and variety screening of submergence tolerance of different genotypes of direct seeding rice at the seedling stage can provide a theoretical basis for further clarifying the mechanism of rice submergence tolerance, screening suitable direct seeding varieties and cultivating special varieties of direct seeding rice.
Cite this paper
Su, X. , Wu, H. , Xiang, J. , Zhan, J. , Wang, J. , Li, X. , Wei, Y. , Dai, H. and Chen, H. (2022). Evaluation of Submergence Tolerance of Different Rice Genotypes at Seedling Emergence Stage under Water Direct Seeding. Open Access Library Journal, 9, e8706. doi: http://dx.doi.org/10.4236/oalib.1108706.
Wang, Y., Wang, Y.Y. and Hong, D.L. (2009) Genetic Variation of Seed Vigor and Tolerance to Anoxia among Rice (Oryza sativa L.) Varieties in Taihu Lake Region. Journal of Nanjing Agricultural University, 32, 1-7.
Gibbs, J. (2000) Regulation of Alcoholic Fermentation in Coleoptiles of Two Rice Cultivars Differing in Tolerance to Anoxia. Journal of Experimental Botany, 51, 785-796. https://doi.org/10.1093/jexbot/51.345.785
Matsushima, K. and Sakagami, J. (2013) Effects of Seed Hydropriming on Germination and Seedling Vigor during Emergence of Rice under Different Soil Moisture Conditions. American Journal of Plant Sciences, 4, 1584-1593.
https://doi.org/10.4236/ajps.2013.48191
Turner, F.T., Cy-Chain, C. and Mccauley, G.N. (1981) Morphological Development of Rice Seedlings in Water at Controlled Oxygen Levels. Agronomy Journal, 73, 566-568. https://doi.org/10.2134/agronj1981.00021962007300030037x
Ismail, A.M., Ella, E.S. and Vergara, G.V. (2009) Mechanisms Associated with Tolerance to Submergence during Germination and Early Seedling Growth in Rice (Oryza sativa). Annals of Botany, 103, 197-209. https://doi.org/10.1093/aob/mcn211
Magneschi, L., Kudahettige, R.L. and Alpi, A. (2009) Comparative Analysis of Anoxic Coleoptile Elongation in Rice Varieties: Relationship between Coleoptile Length and Carbohydrate Levels, Fermentative Metabolism and Anaerobic Gene Expression. Plant Biology, 11, 561-573. https://doi.org/10.1111/j.1438-8677.2008.00150.x
Xiang, J., Wu, H. and Zhang, Y.P. (2017) Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence. International Journal of Molecular Sciences, 18, Article 2225. https://doi.org/10.3390/ijms18102225
Wu, H., Xiang, J., Chen, H.Z., Zhang, Y.P., Zhang, Y.K. and Zhu, D.F. (2018) Effects of Exogenous Growth Regulators on Plant Elongation and Carbohydrate Consumption of Rice Seedlings under Submergence. Chinese Journal of Applied Ecology, 29, 149-157.
Wu, H., Hou, L.L., Zhou, Y.F., Fan, Z.C., Shi, J.Y. and Zhang, J.S. (2012) Analysis of Chilling-Tolerance and Determination of Chilling-Tolerance Evaluation Indicators in Cotton of Different Genotypes. Agricultural Science & Technology, 13, 2338-2346.
Zhao, L.P., Tao, Y.S., Tang, Y.P. and Tang, Q.Y. (2013) The Evolution History and Development Trend of Rice’s Cultivation Methods. Crop Research, 27, 169-173.
Rao, A.N., Johnson, D.E. and Sivaprasad, B. (2007) Weed Management in Direct-Seeded Rice. Advances in Agronomy, 93, 155-255.
https://doi.org/10.1016/S0065-2113(06)93004-1
Iqbal, M.F., Hussain, M. and Rasheed, A. (2017) Direct Seeded Rice: Purely a Site Specific Technology. International Journal of Advanced Research in Biological Sciences, 4, 53-57.
Liu, G.C. and Sun, T.Q. (2012) Analysis on Production Status of Direct-Seeded Rice in Jiangsu Province and Discussion on Countermeasures. China Seed Industry, 31, 44-45.
Ye, T., Qian, C. and Peng, S. (2016) Lower Global Warming Potential and Higher Yield of Wet Direct-Seeded Rice in Central China. Agronomy for Sustainable Development, 36, Article No. 24. https://doi.org/10.1007/s13593-016-0361-2
Wu, X.Z. and Zeng, Q.S. (2017) Development Status, Existing Problems and Countermeasures of Direct Seeding Rice in Hubei Province. Rural Economy and Science-Technology, 28, 154-155.
