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Teratogenic Investigation of Bay Leaf (Syzygium polyanthum Wight.) Ethanol Extract on Morphology of Fetal Mice (Mus musculus L.) Strain DDY

DOI: 10.4236/oalib.1108385, PP. 1-13

Subject Areas: Toxicology

Keywords: Fetus, Mice, Organogenesis, Resorption, S. polyanthum

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Abstract

This research was conducted to observe the teratogenic potential of S. polyanthum ethanol extract on morphology of fetal mice strain DDY. Thirty pregnant female mice were divided into 5 groups, normal group and treatment groups with dose 0.5; 5; 50; 500 mg/bw. The extract was given orally from 6th to 15th day of gestation. The results showed that the effect of S. polyanthum ethanol extract at a dose of 0.5 mg/bw did not cause resorption and external malformation. At a dose of 5 mg/bw, there were resorption (6.15%) and fetal open eyelids (1.63%). Resorption (7.69%; 9.34%) and fetal hemorrhage (1.63%; 1.47%) were found in mice given doses of 50 and 500 mg/bw. The treatment of S. polyanthum ethanol extract at doses 0.5; 5; 50; 500 mg/bw during the period of organogenesis did not have a significant influence on morphology of fetal mice strain DDY (p > 0.05).

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Angelina, M. , Ovy, A. , Dewijanti, I. D. , Mardhiyah, A. and Sjahfirdi, L. (2022). Teratogenic Investigation of Bay Leaf (Syzygium polyanthum Wight.) Ethanol Extract on Morphology of Fetal Mice (Mus musculus L.) Strain DDY. Open Access Library Journal, 9, e8385. doi: http://dx.doi.org/10.4236/oalib.1108385.

References

[1]  Kemenkes, R.I. (2010) Demam Berdarah Dengue. Bulletin Jendela Epidemiologi, 2, 48.
[2]  Sigarlaki, H.J.O. (2007) Karakteristik, pengetahuan, dan sikap ibu terhadap penyakit demam berdarah dengue. Berita Kedokteran Masyarakat, 23, 148-153.
[3]  Widyawati, T. and Ilham, R. (2020) The Effect of Syzygium polyanthum Wight Ethanolic Leaf Extract on Aedes spp Instar III-IV Larvae. Proceedings of the 2nd International Conference on Tropical Medicine and Infectious Disease (ICTROMI), Medan, 5-6 July 2019, 185-188. https://doi.org/10.5220/0009863001850188
[4]  Rosmalena, R., Elya, B., Dewi, B.E., Fithriyah, F., Desti, H., Angelina, M., et al. (2019) The Antiviral Effect of Indonesian Medicinal Plant Extracts against Dengue Virus in Vitro and in Silico. Pathogens, 8, Article No. 85. https://doi.org/10.3390/pathogens8020085
[5]  Dwiyanti, R.D., Dediq, R. and Thuraidah, A. (2017) Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp. Medical Laboratory Technology Journal, 3, 93-97. https://doi.org/10.31964/mltj.v3i1.151
[6]  Ismail, A. and Wan Ahmad, W.A.N. (2019) Syzygium polyanthum (Wight) Walp: A Potential Phytomedicine. Pharmacognosy Journal, 11, 429-438. https://doi.org/10.5530/pj.2019.11.67
[7]  Murray, S.A., Morgan, J.L., Kane, C., Sharma, Y., Heffner, C.S., et al. (2010) Mouse Gestation Length Is Genetically Determined. PLoS ONE, 5, e12418. https://doi.org/10.1371/journal.pone.0012418
[8]  Angelina, M., Dewijanti, I.D., Hartati, S. and Meilawati, L. (2014) Laporan kegiatan kompetitif LIPI pengembangan antiviral dengue dari ekstrak bahan alam. LIPI, Serpong, 108 p.
[9]  Dwianto, W., Amin, Y., Darmawan, T. and Wahyuni, I. (2008) Observasi Jenis-Jenis Kayu Cepat Tumbuh di Kebun Raya Purwodadi dan Ekakarya Bali. RIMBA Kalimantan, 13, 74-77.
[10]  Ecobichon, D.J. (1997) The Basis of Toxicity Testing. CRC Press, Boca Raton, 211 p.
[11]  BPOM (Badan Pengawas Obat dan Makanan) Republik Indonesia (2014) Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia tentang pedoman uji toksisitas nonklinik secara in Vivo. Badan Pengawas Obat dan Makanan Republik Indonesia, Jakarta, 165 p.
[12]  Cook, M.J. and Fairweather, F.A. (1968) Methods Used in Teratogenic Testing. Laboratory Animals, 2, 219-228. https://doi.org/10.1258/002367768781082834
[13]  Akbar, D. (2010) Tumbuhan dengan kandungan senyawa aktif yang berpotensi sebagai bahan antifertilitas. Adabia Press, Jakarta.
[14]  Syahrum, M.H. and Kamaludin, T.A. (1999) Reproduksi dan embriologi: Dari satu sel menjadi organisme. Fakultas Kedokteran Universitas Indonesia, Jakarta, 217 p.
[15]  Soemardji, A.A. and Sutedja, L. (2003) Uji Aktivitas Anti Diabetes dari Ekstrak Kulit Jamblang Syzigium cumini pada Mencit Diabetes Aloksan. Laporan terbatas Kerjasama Penelitian Antar Staf UBI Farmakologi-Toksikologi. Departemen Farmasi, FMIPA ITB dan Pusat Penelitian Kimia LIPI, Bandung, 14 p.
[16]  Danneman, P.J., Suckow, M.A. and Brayton, C.F. (2012) The Laboratory Mouse. 2nd Edition, CRC Press, Boca Raton, 256 p. https://doi.org/10.1201/b12900
[17]  Santoso, S. (2015) Menguasai SPSS22 from Basic to Expert Skills. PT Elex Media Komputindo, Jakarta.
[18]  Wiebold, J.L., Stanfield, P.H., Becker, W.C. and Hillers, J.K. (1986) The Effect of Restraint Stress in Early Pregnancy in Mice. Journal of Reproduction and Fertility, 78, 185-192. https://doi.org/10.1530/jrf.0.0780185
[19]  Widyastuti, N., Widiyani, T. and Listyawati, S. (2006) Efek Teratogenik Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) pada Tikus Putih (Rattus norvegicus) Galur Winstar. Bioteknologi, 3, 56-62.
[20]  Wernsing, D.R. (1999) Fetal and Placental Development in GHR-KO Mice. Honors Thesis, Paper 78, 26 p.
[21]  Depkes, R.I. (2006) Pedoman Pelayanan Farmasi untuk Ibu Hamil dan Menyusui. Direktorat Bina Farmasi Komunitas dan Klinik Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan RI. Jakarta, 53 p.
[22]  Hagerman, A.E. (2002) Tannin Chemistry. Miami University, Oxford, OH, 116 p.
[23]  Baines, M.G., Haddad, E.K., Pomerantz, D.K. and Duclos, A.J. (1994) Effects of Sensory Stimuli on the Incidence of Fetal Resorption in a Murine Model of Spontaneous Abortion: The Presence of an Alien Male and Postimplantation Embryo Survival. Journal of Reproduction and Fertility, 102, 221-228. https://doi.org/10.1530/jrf.0.1020221
[24]  Zhao, Y.T., Wang, X.D., Shi, W.Y. and Zhong, X.H. (2011) Anti-Abortive Effect of Quercetin and Bornyl Acetate on Macrophages and IL-10 in Uterus of Mice. African Journal of Biotechnology, 10, 8675-8682. https://doi.org/10.5897/AJB11.939
[25]  Arck, P.C., Merali, F.S., Stanisz, A.M., Stead, R.H., Chaouat, G., Manuel, J., et al. (1995) Stress-Induced Murine Abortion Associated with Substance P-Dependent Alteration in Cytokines in Maternal Uterine Decidua. Biology of Reproduction, 53, 814-819. https://doi.org/10.1095/biolreprod53.4.814
[26]  Lea, R.G., McIntyre, S., Baird, J.D. and Clark, D.A. (1998) Tumor Necrosis Factor-α mRNA-Positive Cells in Spontaneous Resorption in Rodents. American Journal of Reproductive Immunology, 39, 50-57. https://doi.org/10.1111/j.1600-0897.1998.tb00333.x
[27]  Theiler, K. (1989) A House Mouse: Atlas of Embryonic Development. Springer-Verlag. New York. 178 p. https://doi.org/10.1007/978-3-642-88418-4
[28]  Vanhees, K., Godschalk, R.W., Sanders, A., Van Waalwijk van Doorn-Khosrovani, S.B. and Van Schooten, F.J. (2011) Maternal Quercetin Intake during Pregnancy Results in an Adapted Iron Homeostasis at Adulthood. Toxicology, 290, 350-358. https://doi.org/10.1016/j.tox.2011.10.017
[29]  Setyawati, I., Oka, I.G.L., Putra, S. and Mahardika, I.G. (2014) Calliandra Leaf Meal Decreased Reproductive Performance of Wistar Rat during Gestation and Lactation. International Journal of Pure & Applied Bioscience, 2, 209-214.
[30]  Kalter, H. (1978) Elemination of Fetal Mice with Sporadic Malformation by Spontaneous Resorption in Pregnancies of Older Females. Journal of Reproduction and Fertility, 53, 407-410. https://doi.org/10.1530/jrf.0.0530407

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