Liquid-liquid equilibrium data for aqueous biphasic system formed by polyethylene glycol (PEG)/sodium citrate (C6H5O7Na3)/water (H2O) were determined at 298.15 K, respectively. Binodal data were correlated using a four- parameter equation. The effect of the molecular weight of PEG (1500, 3000, 6000) on the binodal curves was investigated, and it was found that the two-phase region expands with an increase in the molecular weight of PEG. The effects of some monohydric (methanol, ethanol, propanol) and polyhydric alcohols (ethylene glycol, glycerin) on the formation of biphasic system PEG/sodium citrate were examined simultaneously. It was found that the phase diagram binodal in the presence of ethylene glycol and glycerin to the system, obtained that binodal curve is slipped to the top of coordinate at the high concentrations, two-phase systems occur with a higher concentration of components which formed phases. This means that ethylene glycol and glycerin acts as a factor that destroys the structure of water, breaking the hydrogen bonds between water molecules.
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Shahbazova, G. M. and Masimov, E. A. (2022). Effect of a Various Additivies to the Formation of Aqueous Biphasic System Polyetylenglycole (Peg)/Sodium Citrate/Water. Open Access Library Journal, 9, e8142. doi: http://dx.doi.org/10.4236/oalib.1108142.
Zaslavsky, B.Y., Gulaeva, N.D., Djafarov, S.F., Masimov, E.A. and Miheeva, L.M. (1990) Phase Separation in Aqueous Poly(ethylene glycol)-(NH4)2SO4 Systems and Some Physicochemical Properties of the Phases. Journal of Colloid and Interface Science, 137, 147-156. https://doi.org/10.1016/0021-9797(90)90051-O
Toledo, M.O., Farias, F.O., Igarashi-Mafra, L. and Mafra, M.R. (2019) Salt Effect on Ethanol-Based Aqueous Biphasic Systems Applied to Alkaloids Partition: An Experimental and Theoretical Approach, Journal of Chemical & Engineering Data, 64, 2018-2026. https://doi.org/10.1021/acs.jced.8b01024
Zaslavsky, B.Y., Bagirov, T.O., Borovskaya, A.A., et al. (1986) Aqueous Biphasic Systems Formed by Nonionic Polymers I. Effects of Inorganic Salts on Phase Separation. Colloid and Polymer Science, 264, 1066-1071.
Carvalho, C.P., Coimbra, J.S.R. and Costa, I.A.F., Minim, L.A. and Maffia, M.C. (2008) Influence of the Temperature and Type of Salt on the Phase Equilibrium of peg 1500 Potassium Phosphate and peg 1500 Sodium Citrate Aqueous Two-Phase Systems. Química Nova, 31, 209-213.
Ruiz-Ruiz, F., Benavides, J., Aguilar, O. and Rito-Palomares, M. (2012) Aqueous Two-Phase Affinity Partitioning Systems: Current Applications and Trends. Journal of Chromatography A, 1244, 1-13. https://doi.org/10.1016/j.chroma.2012.04.077
Espitia-Saloma, E., Vázquez-Villegas, P., Aguilar, O. and Rito-Palomares, M. (2014) Continuous Aqueous Two-Phase Systems Devices for the Recovery of Biological Products. Food and Bioproducts Processing, 92, 101-112.
Zafarani-Moattar MT, Hamzehzadeh S, Nasiri S. (2012) A New Aqueous Biphasic System Containing Polypropylene Glycol and a Water-Miscible Ionic Liquid. Biotechnology Progress, 28, 146-156. https://doi.org/10.1002/btpr.718
Glyk, A., Solle, D., Scheper, T. and Beutel, S. (2015) Optimization of PEG-Salt Aqueous Two-Phase Systems by Design of Experiments. Chemometrics and Intelligent Laboratory Systems, 149, 12-21. https://doi.org/10.1016/j.chemolab.2015.09.014
Masimov, E., Abbasov, H., Bagirov, T., Hasanova, H. and Gurbanov, J.T. (2007) Structural Features of the Two-Phase System Polyethylene Glycol-С4О6Н4Na2-H2O. Journal Physics Institute of Physics of the National Academy of Sciences of Azerbaijan, 13, 334-336.
Mazzola, P.G., Lopes, A.M., Hasmann, F.A., Jozala, A.F., Penna, T.C., Magalhaes, P.O., Rangel-Yagui, C.O. and Pessoa Jr., A. (2008) Liquid-Liquid Extraction of Biomolecules: An Overview and Update of the Main Techniques. Journal of Chemical Technology & Biotechnology, 83, 143-157. https://doi.org/10.1002/jctb.1794
Van Berlo, M., Luyben, K.C.A. and van der Wielen, L.A. (1998) Poly(ethylene glycol)-Salt Aqueous Two-Phase Systems with Easily Recyclable Volatile Salts. Journal of Chromatography B: Biomedical Sciences and Applications, 711, 61-68.
Krasilnikov, O.V., Sabirov, R.Z., Ternovsky, V.I., Merzliak, P.G., Muratkhodjaev, J.N. (1992, September) A Simple Method for the Determination of the Pore Radius of Ion Channels in Planar Lipid Bilayer Membranes. FEMS Microbiology Immunology, 5, 93-100. https://doi.org/10.1111/j.1574-6968.1992.tb05891.x
Bárcena-Uribarri, I., Thein, M., Maier, E., Bonde, M., Bergström, S. and Benz, R. (2013) Use of Nonelectrolytes Reveals the Channel Size and Oligomeric Constitution of the Borrelia burgdorferi P66 Porin. PLoS ONE, 8, e78272.
Masimov, E.A., Shahbazova, G.M., Bagirov, T.O. and Odzhagverdieva, S.Y. (2017) Influence of Some Monohydryc Alcohols on the Phase Diagram “Peg-Sodium Salt of Citric Acid”. International Journal of Applied and Fundamental Research, 9, 140-142.