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The Influence of Inorganic Salts on the Phase Diagram and Separating Ability of Aqueous Biphasic System: Peg/Sodium Citrate-Water

DOI: 10.4236/oalib.1108105, PP. 1-11

Subject Areas: Biophysics, Physical Chemistry

Keywords: PEG, Sodium Citrate, Biphasic Systems, Separating Ability, Inorganic Salts

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The purpose of the proposed research is to ensure that the value of the distinction is adjusted to each application by using various supplements to separate and clean up biological objects. The work presents the results of studies of the phase diagram of the biphasic system composed of polyethylene glycol (PEG)/sodium citrate-water and the effect of inorganic salts (sodium sulfate, sodium carbonate, sodium nitrate, potassium sulfate, potassium chloride, potassium iodide, potassium bromide) on the separating ability of this biphasic system. Analysis of the data presented shows that the change in the parameters of the phase diagram and different values of the separating ability (for PEG—sodium citrate-water system n* = 9.3) of the biphasic system, depends on the nature of the additives. The utilized inorganic salts change the structure of water clusters. Following the change, the biphasic system component relative hydrophobicity rises, which results in phase separation. The displacements of the binodal of the phase diagram in the direction of the origin of coordinates, an increase in the area of the heterogeneous region of the diagram upon the introduction of the studied salts indicate that these salts have a structuring effect on the aqueous medium.

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Gunel, S. M. and Eldar, M. A. (2021). The Influence of Inorganic Salts on the Phase Diagram and Separating Ability of Aqueous Biphasic System: Peg/Sodium Citrate-Water. Open Access Library Journal, 8, e8105. doi:


[1]  Albertson, P. (1970) Partition of Cell Particles and Macromolecules in Polymer Two- Phase Systems. Advances in Protein Chemistry, 24, 309-341.
[2]  Masimov, E., Bagirov, T., Mahmudov, A., Zaslavski, B. (2018) Phase Separation in Liquid Solutions. Journal of Baku Engineering University, 21, 77-88.
[3]  Masimov, E.A., Hasanov, A.A. and Hasanova, H.T. (2015) “Structural Temperature” and Free Energy of Activation of Viscous Flow of Aqueous Solutions. International Journal of Applied and Fundamental Research, 4, 40-44.
[4]  Masimov, E.A., Ismailov, E.H. and Odzhaqverdiyeva, S.Y. (2015) Complexation of Polyethylene-Glycol with the Sodium Salts of Citric and Succinic Acids in the Aqueous Solutions. Studies by Dynamic Light Scattering and UV/VIS Spectrophotometry. Journal of Advances in Chemistry, 11, 3866-3872
[5]  Zaslavsky, B.Y., Bagirov, T.O., Borovskaya, A.A., et al. (1989). Structure of Water as a Key Factor of Phase Separation in Aqueous Mixtures of Two Nonionic Polymers. Polymer, 30, 2104-2111.
[6]  Albertson, P.-A. (1958) Particle Fractionation in Liquid Two-Phase Systems the Composition of Some Phase Systems and the Behaviour of Some Model Particles in Them Application to the Isolation of Cell Walls From Microorganisms. Biochimica et Biophysica Acta, 27, 378-394.
[7]  Zaslavsky, B.Y. and Masimov, E.A. (1988) Methods of Analysis of the Relative Hydrophobicity of Biological Solutes. In: Physical Organic Chemistry, Vol. 146, Springer, Berlin, Heidelberg, 171-202.
[8]  Raja, S. and Murty, V.R. (2013) Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology. Journal of Engineering, 2013, Article ID: 217483.
[9]  Mazzola, P.G., Lopes, A.M., Hasmann, F.A., Jozala, A.F., Penna, T.C., Magalhaes, P.O., Rangel-Yagui, C.O., Pessoa Jr., A. (2008) Liquid-Liquid Extraction of Biomolecules: An Overview and Update of the Main Techniques. Journal of Chemical Technology & Biotechnology, 83, 143-157.
[10]  Chaiko, D.J., Zaslavsky, B., Rollins, A.N., Vojta, Y., Gartelmann, J. and Mego, W. (1996) Metal Separations Using Aqueous Biphasic Partitioning Systems. Office of Scientific and Technical Information, United States.
[11]  Iqbal, M., Tao, Y., Xie, S., Zhu, Y., Chen, D., Wang, X., et al. (2016) Aqueous Two- Phase System (ATPS): An Overview and Advances in Its Applications. Biological Procedures Online, 18, Article No. 18.
[12]  Da Silva, L.H.M. and Loh, W. (2006) Aqueous Two-Phase Systems: Fundamentals and Applications for Partitioning/Purification of Proteins. Química Nova, 29, 1345- 1351.
[13]  Zafarani-Moattar, M.T., Hamzehzadeh, S. and Nasiri, S. (2012) A New Aqueous Biphasic System Containing Polypropylene Glycol and a Water-Miscible Ionic Liquid. Biotechnology Progress, 28, 146-156.
[14]  Barani, A., Pirdashti, M., Heidari, Z. and Dragoi, E.-N. (2018) Influence of the Molecular Weight of Polymer, Temperature and pH on Phase Diagrams of Poly (Ethylene Glycol) di-potassium Tartrate Aqueous Two-Phase Systems. Fluid Phase Equilibria, 459, 1-9.
[15]  Barbosa, A.A., Bonomo, R.C.F., Martins, C.V., Fontan, R.C.I., Júnior, E.C.S., Minim, L.A. and Pignata, M.C. (2016) Equilibrium Data and Physical Properties of Aqueous Two-Phase Systems Formed by PEG (1500 and 4000) g·mol–1 Sodium Sulfate Water at Different Temperatures and pH 2. Journal of Chemical & Engineering Data, 61, 3-11.
[16]  Han, J., Wang, Y., Yu, C., Li, Y., Kang, W. and Yan, Y. (2012) (Liquid liquid) Equilibrium of (Imidazolium ioNic Liquids Organic Salts) Aqueous Two-Phase Systems at T = 298.15 K and the Influence of Salts and Ionic Liquids on the Phase Separation. The Journal of Chemical Thermodynamics, 45, 59-67.
[17]  Liu, Y., Wu, Z. and Zhao, Y (2015) Liquid-Liquid Equilibrium Correlation of Aqueous Two-Phase Systems Composed of Polyethylene Glycol and Nonionic Surfactant. Thermochimica Acta, 602, 78-86.
[18]  Raja, S. and Murty, V.R. (2013) Liquid-Liquid Equilibrium of Poly (Ethylene Glycol) 6000 Sodium Succinate Water System at Different Temperatures. The Scientific World Journal, 2013, Article ID: 819259.
[19]  Ferreira, L.A. and Teixeira, J.A. (2011) Salt Effect on the Aqueous Two-Phase System PEG 8000-Sodium Sulfate. Journal of Chemical & Engineering Data, 56, 133- 137.
[20]  Zaslavsky, B.Yu., Bagirov, T.O., Borovskaya, A.A., et al. (1986) Aqueous Biphasic Systems Formed by Nonionic Polymers I. Effects of Inorganic Salts on Phase Separation. Colloid and Polymer Science, 264, 1066-1071.
[21]  Zaslavski, B.Y., Mahmudov, A.U., Bagirov, T.O., Borovskaya, A.A. and Rodnikova, M.N. (1987) Aqueous Biphasic Systems Formed by Nonionic Polymers II. Concen- tration Effects of Inorganic Salts of Phase Separation. Colloid and Polymer Science, 265, 548-552.
[22]  Zhang, K., Su, T., Cheng, F., Lin, Y., Zhou, M., Zhu, P., Li, R., Wu, D. (2020) Effect of Sodium Citrate/Polyethlene Glycol on Plasticization and Retrogradation of Maize Stratch. International Journal of Biological Macromolecules, 154, 1471-1477.
[23]  Samoilov, O.Y. (1967) Structure of Water Solutions of Electrolytes. USSR Art. 15- 18, 1967 (in Russian).


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