In the last decade Stevia rebaudiana, has become one of the most demanded crops in the market dedicated to health care and food. However, the main consuming countries, from Asia and Europe, are constantly demanding organic products. Therefore, the purpose of this study was to evaluate the effect of organic fertilizers in combination with biofertilizers in the growth of Stevia. A randomized block design with divided plots (large and small) and three replications were used. In the large plots, the cuttings of stevia were inoculated with 1) Rhizophagus intraradices fungus (Rhiz); 2) A mixture of bacterias Bacillusspp. (Bacl) and Azospirillum brasilense (Azo); 3) Trichoderma spp. fungus (Trch); 4) A treatment with no inoculant (c). These inoculation treatments were combined, in the smaller plots with three sources of organic fertilizers named: 1) Sheep manure (Shim); 2) Bocashi (Bcsh); 3) chicken manure (Chm); 4) A treatment with any organic fertilizer. The plot with no inoculants nor organic fertilizers was the Absolute Control (Ac). There was a positive relationship between organic fertilizers and biofertilizers in the growth of Stevia. The highest yield corresponded to the Rhiz Bcsh with 450.00 kg·ha-1 followed by Rhiz Shim, and Trch Chmboth with 410.00 kg·ha-1. The treatment with the lowest yield was the Ac with 120.00 kg·ha-1.
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Lozano-Contreras, M. G. , Ramírez-Jaramillo, G. and Ramírez-Silva, J. H. (2021). Interaction Effects between Organic Fertilizers and Biofertilizers on the Growth of Stevia rebaudiana Bertoni. Open Access Library Journal, 8, e8065. doi: http://dx.doi.org/10.4236/oalib.1108065.
Nieto-Garibay, A., Murillo-Amador, B., Troyo-Diéguez, E., Larrinaga-Mayoral, J.á. and García-Hernández, J.L. (2002) El uso de compostas como alternative ecológica para la producción sostenible del chile (Capsicum annuum L.) en zonas áridas. Interciencia, 27, 417-421. https://www.redalyc.org/pdf/339/33907106.pdf
Martinez, C.C. (2003) Abonos Orgánicos: Origen, Usos y Aplicación. Secretaría de Desarrollo Social del Gobierno del Estado de Chiapas. Dirección de Promoción Social. Chiapas, México, 14 p.
Hernández Garciadiego, R. and Herrerías Guerra, G. (1998) Amaranto: Historia y Promesa. Tehuacán: Horizonte del Tiempo. Vol. 1 Patrimonio Histórico de Tehuacán A. C, México, 529 p. http://www.alternativas.org.mx/Amaranto.pdf
álvarez Solís, J.D., Ferrera Cerrato, R. and Etchevers Barra, J.D. (2000) Actividad microbiana en tepetate con incorporación de residues orgánicos. Agrociencia, 34, 523-532. https://www.redalyc.org/articulo.oa?id=30234501
Pansu, M., Sallih, Z. and Bottner, P. (1998) Modelling of Soil Nitrogen Forms after Organic Amendments under Controlled Conditions. Soil Biology and Biochemistry, 30, 19-29. https://doi.org/10.1016/S0038-0717(97)00087-4
https://www.sciencedirect.com/science/article/abs/pii/S0038071797000874
Ruíz, F.J.F. (1996) Los fertilizantes y la fertilizaciónorgánica bajo la óptica de un sistema de producción orgánica. En Altamirano, Z. and Arózqueta, C., Eds., Memorias Primer Foro Nacional sobre Agricultura Orgánica, Universidad Autónoma Metropolitana, Unidad Xochimilco, México, DF, 23-47.
Abdel Magid, H.M., Sabrah, R.E.A., El Nadi, A.R.H., Abdel-Aal, S.I. and Rabic, R.K. (1994) Kinetics of Biodegradation Bates of Chicken Manure and Municipal Refuse in a Sandy Soil. Journal of Arid Environments, 28, 163-171.
https://doi.org/10.1016/S0140-1963(05)80047-3
Rodríguez Dimas, N., Cano Ríos, P., Figueroa Viramontes, U., Favela Chávez, E., Moreno Reséndez, A., Márquez Hernández, C., Ochoa Martínez, E. and Preciado Rangel, P. (2009) Uso de abonos orgánicos en la producción de tomate en invernadero. Terra Latinoamericana, 27, 319-327.
https://www.redalyc.org/pdf/573/57313040006.pdf
https://www.researchgate.net/profile/Candido-Marquez/publication/264274248_Uso_de_abonos_organicos_en_la_produccion_de_tomate_en_invernadero/links/53d684d90cf228d363ea5d90/Uso-de-abonos-organicos-en-la-produccion-de-tomate-en-invernadero.pdf
Rueda, P.E.O., Murillo, A.B., García, H.J.L., Barrón, H.J.M., Preciado, R.P. and Tarazón, H.M.A. (2009) Las bacterias promotoras del crecimiento vegetal como biofertilizantes en ambientes árido salinos. In: Castillo, I.O., Salazar, E., Fortis, M., Trejo, H.I., Vázquez, C., López, J.D., Figueroa, R., Zúñiga, R., Preciado, P. and Chavarría, J.A., Eds., agricultura orgánica, Comisión Nacional de la Ciencia del Suelo y CONACYT. Gómez Palacio, México, 259-272.
Caballero-Mellado, J., Carcaño-Montiel, M.G. and Mascarua-Esparza, M.A. (1992) Field Inoculation of Wheat (Triticum aestivum) with Azospirillum brasilense under Temperate Climate. Symbiosis, 13, 243-253.
https://dalspace.library.dal.ca/bitstream/handle/10222/77357/VOLUME%2013-NUMBERS%201,2,3-1992-PAGE%20243.pdf?sequence=1
Caballero-Mellado, J., Onofre-Lemus, J., Estrada-de los Santos, P. and Martínez- Aguilar, L. (2007) The Tomato Rhizosphere, an Environment Rich in Nitrogen fixing burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation. Applied and Environmental Microbiology, 73, 5308-5319.
https://doi.org/10.1128/AEM.00324-07
Mendoza, H.A. and Cruz, H.M.A. (2010) Empleo de Azospirillum Comobiofertilizante. In: Aguado, G.A., Ed., Introducción al uso y manejo de los biofertilizantes en la agricultura. INIFAP-SAGARPA, México, 171-185.
Umesha, S., Divya, M., Prasanna, K.S., Lakshmipathi, R.N. and Sreeramulu, K.R. (2014) Comparative Effect of Organics and Biofertilizers on Growth and Yield of Maize (Zea mays L.). Current Agriculture Research Journal, 2, 55-62.
https://doi.org/10.12944/CARJ.2.1.08
Geuns, J.M.C, Augustijns, P., Mols, R., Buyse, J.G. and Driessen, B. (2003) Metabolism of Stevioside in Pigs and Intestinal Absorption Characteristics of Stevioside, Rebaudioside A and Steviol. Food and Chemical Toxicology, 41, 1599-1607.
https://doi.org/10.1016/S0278-6915(03)00191-1
https://www.sciencedirect.com/science/article/abs/pii/S0278691503001911
Grosso, V., Podetti, J. and Soltermann, A. (2012) Stevia rebaudiana Bertoni. Cuadernillo de Divulgación del Laboratorio de Desarrollo y Vinculacion Tecnologica. Colección vinculación y Educación. Universidad Nacional de Río Cuarto Ruta Nacional. Río Cuarto, Argentina. UniRio Editora, 24 p.
Christaki, E., Bonos, E., Giannenas, I., Karatzia, M.A. and Florou-Paneri, P.C. (2013) Stevia rebaudiana as a Novel Source of Food Additives. Journal of Food and Nutritional Research, 52, 195-202.
Chaturvedula, V.S.P. and Zamora, J. (2014) Reversed-Phase HPLC Analysis of Steviol Glycosides Isolated from Stevia rebaudiana Bertoni. Food and Nutrition Sciences, 5, 1711-1716. https://doi.org/10.4236/fns.2014.517184
Shintani, M., Leblanc, H. and Tabora, P. (2000) Bokashi, Abono orgánico fermentado; Tecnología Tradicional Adaptada para una Agricultura Sostenible y un Manejo de Desechos Modernos. (1 Ed.). Escuela de Agricultura de la Región Tropical Humedad. San José, Costa Rica, 14-25.
Uribe-Valle, G. and Dzib-Echeverría, R. (2006) Micorriza arbuscular (Glomus intraradices), Azospirillum brasilense y Brassinoesteroide en la producción de maízen el suelo luvisol. Agricultura Técnica en México, 32, 67-76.
https://www.redalyc.org/pdf/608/60832107.pdf
Lozano-Contreras, M.G. and Ramírez-Jaramillo, G. (2015) Enraizamiento de esquejes de estevia con biofertilizantes. Desplegable para productores No. 6, Centro de investigación regional sureste. Campo experimental Mocochá. Mérida, Yucatán.
Daniels-Hylton, K.D.M. and Ahmad, M.H. (1994) Inoculation Response in Kidney Beans (Phaseolus vulgaris L.) to Vesicular-Arbuscular Mycorrhizal Fungi and Rhizobia in Non-Sterilized Soil. Biology and Fertility of Soils, 18, 95-98.
https://link.springer.com/article/10.1007/BF00336452
Allen, B.L., Jolley, V.D., Robbins, C W. and Freeborne, L.L. (2001) Fallow versus Wheat Cropping of Unamended and Manure-Amended Soils Related to Mycorrhizal Colonization, Yield and Plant Nutrition of Dry Bean and Sweet Corn. Journal of Plant Nutrition, 24, 921-943. https://doi.org/10.1081/PLN-100103782
Hernández-León, R., Rojas-Solís, D., Contreras-Pérez, M, Orozco-Mosqueda, M.D.C., Macías-Rodríguez, L.I., Reyes-de la Cruz, H., Valencia-Cantero, E. and Santoyo, G. (2015) Characterization of the Antifungal and Plant Growth-Promoting Effects of Diffusible and Volatile Organic Compounds Produced by Pseudomonas fluorescens Strains. Biological Control, 81, 83-92.
https://doi.org/10.1016/j.biocontrol.2014.11.011
Domingues Duarte, C.F., Cecato, U., Trento Biserra, T., Mamédio, D. and Galbeiro, S. (2020) Azospirillum spp. engramíneas y forrajeras. Revisión. Revista mexicana de ciencias pecuarias, 11, 223-240. https://doi.org/10.22319/rmcp.v11i1.4951
Kashyap, P.L., Rai, P., Srivastava, A.K. and Kumar, S. (2017) Trichoderma for Climate Resilient Agriculture. World Journal of Microbiology Biotechnology, 33, Article No. 155. https://doi.org/10.1007/s11274-017-2319-1
Villamarín-Gallegos, D., Oviedo-Pereira, D.G., Evangelista-Lozano, S., Sepúlveda- Jiménez, G., Molina-Torres, J. and Rodríguez-Monroy. M. (2020) Trichoderma asperellum, an Inoculant for the Production of Steviol Glycosides in Stevia rebaudiana Bertoni Plants Micropropagated in a Temporary Immersion Bioreactor. Revista Mexicana de Ingeniería Química, 19, 1153-1161.
https://doi.org/10.24275/rmiq/Bio947
http://rmiq.org/ojs311/index.php/rmiq/article/view/947/429
Garcés, K.S.R. (2011) Evaluación de diferentes niveles de abono orgánico solido potencializado con Trichoderma en la producción de Medicago sativa (Alfalfa) en la estación experimental Sunshi. Riobamba, Chimborazo, Ecuador, 76 p
Cegarra, J.A., Roig, A.F., Navarro, M.P., Bernal, M., Abad, M., Climent, D. and Aragón, P. (1993) Características, compostaje y uso agrícola de residues sólidosurbanos. In: Warman, P.R. and Taylor, B.R., eds., Memorias Jornadas de Recogidas Selectivasen Origen y Reciclaje, Mundi-Prensa, Córdoba, España, 46-55.
Ouédraogo, E., Mando, A. and Zombré, N.P. (2001) Use of Compost to Improve Soil Properties and Crop Productivity under Low Input Agricultural System in West Africa. Agriculture, Ecosystems & Environment, 84, 259-266.
https://doi.org/10.1016/S0167-8809(00)00246-2
https://www.sciencedirect.com/science/article/abs/pii/S0167880900002462
Courtney, R.G. and Mullen, G.J. (2008) Soil Quality and Barley Growth as Influenced by the Land Application of Two Compost Types. Bioresource Technology, 99, 2913-2918. https://doi.org/10.1016/j.biortech.2007.06.034
Erhart, E. and Hartl, W. (2003) Mulching with Compost Improves Growth of Blue Spruce in Christmas Tree Plantations. European Journal of Soil Biology, 39, 149-156. https://doi.org/10.1016/S1164-5563(03)00030-X
Jakobsen, S.T. (1996) Leaching of Nutrients from Pots with and without Applied Compost. Resources, Conservation and Recycling, 17, 1-11.
https://doi.org/10.1016/0921-3449(96)01069-5
Miyasaka, S.C., Hollyer, J.R. and Kodani, L.S. (2001) Mulch and Compost Effects on Yield and Corm Rots of Taro. Field Crops Research, 71, 101-112.
https://doi.org/10.1016/S0378-4290(01)00154-X
Sánchez de la Cruz, R., Díaz-Franco, A., Pecina-Quintero, V., Garza-Cano, I. and Loera-Gallardo, J. (2008) Glomus intraradices y Azospirillum brasilense en trigo bajo dos regímenes de humedaden el suelo. Universidad y Ciencia, 24, 239-245.
http://www.scielo.org.mx/pdf/uc/v24n3/v24n3a8.pdf
Aguirre-Medina, J.F. (2006) Biofertilizantes microbianos: Experiencias agronómicas del programa nacional del INIFAP en México. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-Centro de Investigaciones Regionales Pacífico Sur-Campo Experimental Rosario Izapa, México.
Aguirre-Medina, Juan F., Mina-Briones, Francisco O., Cadena-Iñiguez, Jorge, Dardón- Zunun, J.D. and Hernández-Sedas, D.A. (2014) Crecimiento de Cedrelao dorata L. Biofertilizada con Rhizophagus intraradices y Azospirillum brasilense en vivero. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 20, 177-183.
https://doi.org/10.5154/r.rchscfa.2014.01.001
Harman, G.E. (2000) Myths and Dogmas of Biocontrol. Changes in Perceptions Derived from Research on Trichoderma harzianum T-22. Plant Disease, 84, 377-393.
https://doi.org/10.1094/PDIS.2000.84.4.377
Pertot, I., Alaboubette, C., Hinarejos, E. and Franca, S. (2015) Mini Papaer: The Use of Microbial Biocontrol Agents against Soil-Borne Diseases. Eip-Agri, Agriculture & Innovation, Bruselas, Bélgica.
http://ec.europa.eu/eip/agriculture/sites/agrieip/files/8_eip_sbd_mp_biocontrol_final.pdf
Paleologos, M.F. and Sarandón, S.J. (2014) Principios de ecología de poblaciones. En Sarandón, S. J. and Flores, C.C., Eds., Agroecología: bases teóricas para el diseño y manejo de Agroecosistemas sustentables, Edulp. La Plata, Buenos Aires, Argentina, 235-258.
https://aulavirtual.agro.unlp.edu.ar/pluginfile.php/20439/mod_resource/content/1/Capitulo%209%20Ecologia%20de%20poblaciones.pdf
Pirlak, L. and Köse, M. (2009) Effects of Plant Growth Promoting Rhizobacteria on Yield and Some Fruit Properties of Strawberry. Journal of Plant Nutrition, 32, 1173-1184. https://doi.org/10.1080/01904160902943197
Karakurt, H., Kotan, R., Dadasoglu, F., Aslantas, R. and Sahin, F. (2011) Effects of Plant Growth Promoting Rhizobacteria on Fruit Set, Pomological and Chemical Characteristics, Color Values, and Vegetative Growth of Sour Cherry (Prunus cerasus cv. Kütahya). Turkish Journal of Biology, 35, 283-291.
http://supersol.com.tr/Files/PlantNutrition/2011Prunuscerasus.pdf