全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

Prediction of the Active Ingredients and Mechanism of ASH against Liver Cancer Based on Network Pharmacology and Molecular Docking

DOI: 10.4236/oalib.1107739, PP. 1-14

Subject Areas: Medical Genetics, Molecular Biology, Biological Engineering

Keywords: Artemisiae scopariae Herba (ASH), Liver Cancer, Network Pharmacology, Molecular Docking

Full-Text   Cite this paper   Add to My Lib

Abstract

The network pharmacology and molecular docking technology were used to elucidate the mechanism of Artemisiae scopariae Herba (ASH) against liver cancer (LC). TCMSP and UniProt database were used to collect the active ingredients of ASH and predict their potential targets. The targets of LC were screened by GeneCards, OMIM and TTD database. The intersections of drug and disease targets were obtained by online software Venny 2.1, and the intersection targets were imported into R software (v3.6.3) for GO and KEGG function enrichment analysis. Construction of protein-protein interaction (PPI) network through STRING database, Cytoscape software was used to screen hub genes. Molecular docking analysis of hub genes was carried out with AutoDock vina software. A total of 13 active ingredients were screened out from ASH and 103 drug and disease intersection targets were screened. Finally, 7 hub targets including AKT1, TP53, JUN, MAPK1, TNF, RELA, IL6 were screened out. The hub targets were docked well with some active ingredients. The active ingredients of ASH are involved in hepatitis B, hepatitis C and other signaling pathways by acting on AKT1, TP53, JUN and other targets, which may play a role in the treatment of LC.

 Cite this paper

Guo, W. , Zhang, K. and Yang, L. (2021). Prediction of the Active Ingredients and Mechanism of ASH against Liver Cancer Based on Network Pharmacology and Molecular Docking. Open Access Library Journal, 8, e7739. doi: http://dx.doi.org/10.4236/oalib.1107739.

References

[1]  Son, H.U., Lee, S., Heo, J.C. and Lee, S.H. (2017) The Solid-State Fermentation of Artemisia capillaris Leaves with Ganoderma lucidum Enhances the Anti-Inflammatory Effects in a Model of Atopic Dermatitis. International Journal of Molecular Medicine, 39, 1233-1241. https://doi.org/10.3892/ijmm.2017.2945
[2]  Kim, J., Jung, K.H., Yan, H.H., Cheon, M.J., Kang, S., et al. (2018) Artemisia capillaris Leaves Inhibit Cell Proliferation and Induce Apoptosis in Hepatocellular Carcinoma. BMC Complementary and Alternative Medicine, 18, 147. https://doi.org/10.1186/s12906-018-2217-6
[3]  Yan, H., Jung, K.H., Kim, J., Rumman, M., Oh, M.S., et al. (2018) Artemisia capillaris Extract AC68 Induces Apoptosis of Hepatocellular Carcinoma by Blocking the PI3K/AKT Pathway. Biomedicine & Pharmacotherapy, 98, 134-141. https://doi.org/10.1016/j.biopha.2017.12.043
[4]  Jang, E., Kim, B.J., Lee, K.T., Inn, K.S. and Lee, J.H. (2015) A Survey of Therapeutic Effects of Artemisia capillaris in Liver Diseases. Evidence-Based Complementary and Alternative Medicine, 2015, Article ID: 728137. https://doi.org/10.1155/2015/728137
[5]  Tseng, H.C., Xiong, W., Badeti, S., Yang, Y., Ma, M., et al. (2020) Efficacy of Anti-CD147 Chimeric Antigen Receptors Targeting Hepatocellular Carcinoma. Nature Communications, 11, 4810. https://doi.org/10.1038/s41467-020-18444-2
[6]  Yapasert,R., Lertprasertsuk, N., Subhawa, S., Poofery, J., Sripanidkulchai, B., et al. (2020) Antitumor Efficacy of the Herbal Recipe Benja Amarit against Highly Invasive Cholangiocarcinoma by Inducing Apoptosis Both in Vitro and in Vivo. International Journal of Molecular Sciences, 21, 5669. https://doi.org/10.3390/ijms21165669
[7]  Liu, M., Yan, Q., Sun, Y., Nam, Y., Hu, L., et al. (2020) A Hepatocyte Differentiation Model Reveals Two Subtypes of Liver Cancer with Different Oncofetal Properties and Therapeutic Targets. Proceedings of the National Academy of Sciences of the United States of America, 117, 6103-6113. https://doi.org/10.1073/pnas.1912146117
[8]  Anwanwan, D., Singh, S.K., Singh, S., Saikam, V. and Singh, R. (2020) Challenges in Liver Cancer and Possible Treatment Approaches. Biochimica et Biophysica Acta. Reviews on Cancer, 1873, Article ID: 188314. https://doi.org/10.1016/j.bbcan.2019.188314
[9]  Wang, X., Hu, Z., Wang, Z., Cui, Y. and Cui, X. (2019) Angiopoietin-Like Protein 2 Is an Important Facilitator of Tumor Proliferation, Metastasis, Angiogenesis and Glycolysis in Osteosarcoma. American Journal of Translational Research, 11, 6341-6355.
[10]  Tian, X., Wu, Y., Yang, Y., Wang, J., Niu, M., et al. (2020) Long Noncoding RNA LINC00662 Promotes M2 Macrophage Polarization and Hepatocellular Carcinoma Progression via Activating Wnt/β-Catenin Signaling. Molecular Oncology, 14, 462-483. https://doi.org/10.1002/1878-0261.12606
[11]  Hefnawy, A., Khalil, I.H., Arafa, K., Emara, M. and El-Sherbiny, I.M. (2020) Dual-Ligand Functionalized Core-Shell Chitosan-Based Nanocarrier for Hepatocellular Carcinoma-Targeted Drug Delivery. International Journal of Nanomedicine, 15, 821-837. https://doi.org/10.2147/IJN.S240359
[12]  Wang, X., Wang, N., Cheung, F., Lao, L., Li, C., et al. (2015) Chinese Medicines for Prevention and Treatment of Human Hepatocellular Carcinoma: Current Progress on Pharmacological Actions and Mechanisms. Journal of Integrative Medicine, 13, 142-164. https://doi.org/10.1016/S2095-4964(15)60171-6
[13]  Jian, G.H., Su, B.Z., Zhou, W.J. and Xiong, H. (2020) Application of Network Pharmacology and Molecular Docking to Elucidate the Potential Mechanism of Eucommia ulmoides-Radix Achyranthis bidentatae against Osteoarthritis. BioData Mining, 13, 12. https://doi.org/10.1186/s13040-020-00221-y
[14]  Zhang, Y., Tseng, J.T., Lien, I.C., Li, F., Wu, W., et al. (2020) mRNAsi Index: Machine Learning in Mining Lung Adenocarcinoma Stem Cell Biomarkers. Genes, 11, 257. https://doi.org/10.3390/genes11030257
[15]  Fang, J., Pan, Z., Yu, H., Yang, S., Hu, X., et al. (2020) Regulatory Master Genes Identification and Drug Repositioning by Integrative mRNA-miRNA Network Analysis for Acute Type A Aortic Dissection. Frontiers in Pharmacology, 11, Article ID: 575765. https://doi.org/10.3389/fphar.2020.575765
[16]  Cao, F., Guo, Y., Zhang, Q., Fan, Y., Liu, Q., et al. (2020) Integration of Transcriptome Resequencing and Quantitative Proteomics Analyses of Collagenase VII-Induced Intracerebral Hemorrhage in Mice. Frontiers in Genetics, 11, Article ID: 551065. https://doi.org/10.3389/fgene.2020.551065
[17]  Yin, B., Bi, Y.M., Fan, G.J. and Xia, Y.Q. (2020) Molecular Mechanism of the Effect of Huanglian Jiedu Decoction on Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking. Journal of Diabetes Research, 2020, Article ID: 5273914. https://doi.org/10.1155/2020/5273914
[18]  Samec, M., Liskova, A., Koklesova, L., Samuel, S.M., Zhai, K., et al. (2020) Flavonoids against the Warburg Phenotype-Concepts of Predictive, Preventive and Personalised Medicine to Cut the Gordian Knot of Cancer Cell Metabolism. The EPMA Journal, 11, 377-398. https://doi.org/10.1007/s13167-020-00217-y
[19]  Liu, X., Hu, P., Li, H., Yu, X.X., Wang, X.Y., et al. (2020) LW-213, a Newly Synthesized Flavonoid, Induces G2/M Phase Arrest and Apoptosis in Chronic Myeloid Leukemia. Acta Pharmacologica Sinica, 41, 249-259. https://doi.org/10.1038/s41401-019-0270-4
[20]  Liao, C.Y., Lee, C.C., Tsai, C.C., Hsueh, C.W., Wang, C.C., et al. (2015) Novel Investigations of Flavonoids as Chemopreventive Agents for Hepatocellular Carcinoma. BioMed Research International, 2015, Article ID: 840542. https://doi.org/10.1155/2015/840542
[21]  Silva, C.F.M., Batista, V.F., Pinto, D. and Silva, A.M.S. (2018) Challenges with Chromone as a Privileged Scaffold in Drug Discovery. Expert Opinion on Drug Discovery, 13, 795-798. https://doi.org/10.1080/17460441.2018.1494720
[22]  Shahzad, N., Khan, W., Md, S., Ali, A., Saluja, S.S., et al. (2017) Phytosterols as a Natural Anticancer Agent: Current Status and Future Perspective. Biomedicine & Pharmacotherapy, 88, 786-794. https://doi.org/10.1016/j.biopha.2017.01.068
[23]  Gu, Z., Wang, L., Yao, X., Long, Q., Lee, K., et al. (2020) ClC-3/SGK1 Regulatory Axis Enhances the Olaparib-Induced Antitumor Effect in Human Stomach Adenocarcinoma. Cell Death & Disease, 11, 898. https://doi.org/10.1038/s41419-020-03107-3
[24]  Chen, J., Liang, J., Liu, S., Song, S., Guo, W., et al. (2018) Differential Regulation of AKT1 Contributes to Survival and Proliferation in Hepatocellular Carcinoma Cells by Mediating Notch1 Expression. Oncology Letters, 15, 6857-6864. https://doi.org/10.3892/ol.2018.8193
[25]  Yu, Y., Gao, C., Chen, Y., Wang, M., Zhang, J., et al. (2020) Copy Number Analysis Reveal Genetic Risks of Penile Cancer. Frontiers in Oncology, 10, Article ID: 596261. https://doi.org/10.3389/fonc.2020.596261
[26]  Geng, X., Wang, Y., Hong, Q., Yang, J., Zheng, W., et al. (2015) Differences in Gene Expression Profiles and Signaling Pathways in Rhabdomyolysis-Induced Acute Kidney Injury. International Journal of Clinical and Experimental Pathology, 8, 14087-14098.
[27]  Chang, L., Yuan, W. and Zhu, L. (2020) β-Cantenin Is Potentially Involved in the Regulation of c-Jun Signaling Following Bovine Herpesvirus 1 Infection. Veterinary Microbiology, 248, Article ID: 108804. https://doi.org/10.1016/j.vetmic.2020.108804
[28]  Jiang, G., Zhang, L., Zhu, Q., Bai, D., Zhang, C., et al. (2016) CD146 Promotes Metastasis and Predicts Poor Prognosis of Hepatocellular Carcinoma. Journal of Experimental & Clinical Cancer Research, 35, 38. https://doi.org/10.1186/s13046-016-0313-3
[29]  Roderburg, C., Gautheron, J. and Luedde, T. (2012) TNF-Dependent Signaling Pathways in Liver Cancer: Promising Targets for Therapeutic Strategies? Digestive Diseases (Basel, Switzerland), 30, 500-507. https://doi.org/10.1159/000341700
[30]  Pierce, R.H., Campbell, J.S., Stephenson, A.B., Franklin, C.C., Chaisson, M., et al. (2000) Disruption of Redox Homeostasis in Tumor Necrosis Factor-Induced Apoptosis in a Murine Hepatocyte Cell Line. The American Journal of Pathology, 157, 221-236. https://doi.org/10.1016/S0002-9440(10)64533-6
[31]  Tan, W., Luo, X., Li, W., Zhong, J., Cao, J., et al. (2019) TNF-α Is a Potential Therapeutic Target to Overcome Sorafenib Resistance in Hepatocellular Carcinoma. EBioMedicine, 40, 446-456. https://doi.org/10.1016/j.ebiom.2018.12.047
[32]  Luo, Z., Yi, Z.J., Ou, Z.L., Han, T., Wan, T., et al. (2019) RELA/NEAT1/ miR-302a-3p/RELA Feedback Loop Modulates Pancreatic Ductal Adenocarcinoma Cell Proliferation and Migration. Journal of Cellular Physiology, 234, 3583-3597. https://doi.org/10.1002/jcp.27039
[33]  Ricca, A., Biroccio, A., Trisciuoglio, D., Cippitelli, M., Zupi, G., et al. (2001) relA Over-Expression Reduces Tumorigenicity and Activates Apoptosis in Human Cancer Cells. British Journal of Cancer, 85, 1914-1921. https://doi.org/10.1054/bjoc.2001.2174
[34]  Kim, Y., Allen, E., Baird, L.A., Symer, E.M., Korkmaz, F.T., et al. (2019) NF-κB RelA Is Required for Hepatoprotection during Pneumonia and Sepsis. Infection and Immunity, 87, e00132-19. https://doi.org/10.1128/IAI.00132-19
[35]  Chen, S., Tang, Y., Yang, C., Li, K., Huang, X., et al. (2020) Silencing CDC25A Inhibits the Proliferation of Liver Cancer Cells by Downregulating IL-6 in Vitro and in Vivo. International Journal of Molecular Medicine, 45, 743-752. https://doi.org/10.3892/ijmm.2020.4461
[36]  Song, Y., Yang, X., Shen, Y., Wang, Y., Xia, X., et al. (2019) STAT3 Signaling Pathway Plays Importantly Genetic and Functional Roles in HCV Infection. Molecular Genetics & Genomic Medicine, 7, e821. https://doi.org/10.1002/mgg3.821

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133

WeChat 1538708413




©2008-2020 OALib, Inc | Public user content licensed CC-BY 4.0 unless otherwise specified. OALib ISSN Online: 2333-9721 OALib ISSN Print: 2333-9705