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Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties

DOI: 10.4236/oalib.1107291, PP. 1-8

Subject Areas: Bioengineering, Microbiology

Keywords: Bacillus subtilis QM3, Alpha-Amylase, Enzymological Properties

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Alpha-amylase is a starch hydrolyzing enzyme which has many industrial applications. In this study, Bacillus subtilis QM3 has ability to produce α-amylase. Identification was done by iodine test, based on the clear zone around the sample in starch agar plates. Alpha-amylase activity was measured using 3,5-dinitrosalicylic acid (DNS). The result showed B. subtilis QM3 can produce α-amylase and activity of α-amylase was 50.58 IU/mL. The study on enzymatic properties was found: the optimum temperature is 70°C, and its stability is high at 30°C - 70°C. The optimum pH is 6.0, and the stability is high under the condition of pH was 6.0 - 7.0. Ca2 , Na , K and Mg2 promoted the activity of alpha-amylase, and their relative enzyme activities were 130.1%, 118.1%, 115.9% and 110.4% respectively. Fe2 , Zn2 , Cu2 , Mn2 and EDTA inhibited the activity of alpha-amylase. The relative enzyme activities were 86.1% 77.8%, 67.4%, 64.2% and 48.4% respectively.

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Hu, Q. and Liu, J. (2021). Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties. Open Access Library Journal, 8, e7291. doi:


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