Alpha-amylase is a starch hydrolyzing enzyme which has many industrial applications. In this study, Bacillus subtilis QM3 has ability to produce α-amylase. Identification was done by iodine test, based on the clear zone around the sample in starch agar plates. Alpha-amylase activity was measured using 3,5-dinitrosalicylic acid (DNS). The result showed B. subtilis QM3 can produce α-amylase and activity of α-amylase was 50.58 IU/mL. The study on enzymatic properties was found: the optimum temperature is 70°C, and its stability is high at 30°C - 70°C. The optimum pH is 6.0, and the stability is high under the condition of pH was 6.0 - 7.0. Ca2 , Na, K and Mg2 promoted the activity of alpha-amylase, and their relative enzyme activities were 130.1%, 118.1%, 115.9% and 110.4% respectively. Fe2 , Zn2 , Cu2 , Mn2 and EDTA inhibited the activity of alpha-amylase. The relative enzyme activities were 86.1% 77.8%, 67.4%, 64.2% and 48.4% respectively.
Ahmed, M.A., Eman, A.K., Manal, M.G., Maysa, E.M. and Amany, L.K. (2020) Production of a Novel α-Amylase by Bacillus Atrophaeus NRC1 Isolated from Honey: Purification and Characterization. International Journal of Biological Macromolecules, 148, 292-301. https://doi.org/10.1016/j.ijbiomac.2020.01.120
David, S., Femi, B., Gbenga, A. and Adeleke, B.S. (2017) Purification and Characterization of α-Amylase from Bacillus subtilis Isolated from Cassava Processing Sites. Journal of Bioremediation & Biodegradation, 8, 2-7.
Wu, X.R., Wang, Y.X., Tong, B.D., Chen, X.H. and Jian, H. (2018) Purification and Biochemical Characterization of a Thermostable and Acid-Stable Alpha-Amylase from Bacillus licheniformis B4-423. International Journal of Biological Macromolecules: Structure, Function and Interactions, 109, 329-337.
Rajagopalan, G. and Krishnan, C. (2008) Alpha-Amylase Production from Catabolite Derepressed Bacillus subtilis KCC103 Utilizing Sugarcane Bagasse Hydrolysate. Bioresource Technology, 99, 3044-3050.
Jaiswal, N. and Prakash, O. (1998) Immobilization of Soybean a-Amylase on Gelatin and its Application as a Detergent Additive. Asian Journal of Biochemistry, 6, 337-346.
Saideh, A.A., Abdolhamid, B.D. and Namaki, S. (2016) Characterization of a Thermostable, CaCl2-Activated and Raw-Starch Hydrolyzing Alpha-Amylase from Bacillus licheniformis AT70: Production under Solid State Fermentation by Utilizing Agricultural Wastes. Journal of Molecular Catalysis B: Enzymatic, 132, 98-106.
Ahmed, S.A., Abdella, M.A., El-Sherbiny, G.M., Ibrahim, A.M. and Atalla, S.M. (2019) Application of One-Factor-at-a-Time and Statistical Designs to Enhance α-Amylase Production by a Newly Isolate Bacillus subtilis Strain-MK1. Biocatalysis and Agricultural Biotechnology, 22, Article ID: 101397.
Raul, D., Biswas, T., Mukhopadhyay, S., Das, S.K. and Gupta, S. (2014) Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation. Biochemistry Research International, 2014, Article ID: 586141. https://doi.org/10.1155/2014/568141
Li, Y.J. and Hu, Q.P. (2020) Effect of Bacillus subtilis QM3 on β-Amylase Isoenzyme in Early Germination of Wheat Seed. South Asian Journal of Research in Microbiology, 6, 24-32. https://doi.org/10.9734/sajrm/2020/v6i230146