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Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties

DOI: 10.4236/oalib.1107291, PP. 1-8

Subject Areas: Bioengineering, Microbiology

Keywords: Bacillus subtilis QM3, Alpha-Amylase, Enzymological Properties

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Abstract

Alpha-amylase is a starch hydrolyzing enzyme which has many industrial applications. In this study, Bacillus subtilis QM3 has ability to produce α-amylase. Identification was done by iodine test, based on the clear zone around the sample in starch agar plates. Alpha-amylase activity was measured using 3,5-dinitrosalicylic acid (DNS). The result showed B. subtilis QM3 can produce α-amylase and activity of α-amylase was 50.58 IU/mL. The study on enzymatic properties was found: the optimum temperature is 70°C, and its stability is high at 30°C - 70°C. The optimum pH is 6.0, and the stability is high under the condition of pH was 6.0 - 7.0. Ca2 , Na , K and Mg2 promoted the activity of alpha-amylase, and their relative enzyme activities were 130.1%, 118.1%, 115.9% and 110.4% respectively. Fe2 , Zn2 , Cu2 , Mn2 and EDTA inhibited the activity of alpha-amylase. The relative enzyme activities were 86.1% 77.8%, 67.4%, 64.2% and 48.4% respectively.

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Hu, Q. and Liu, J. (2021). Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties. Open Access Library Journal, 8, e7291. doi: http://dx.doi.org/10.4236/oalib.1107291.

References

[1]  Ahmed, M.A., Eman, A.K., Manal, M.G., Maysa, E.M. and Amany, L.K. (2020) Production of a Novel α-Amylase by Bacillus Atrophaeus NRC1 Isolated from Honey: Purification and Characterization. International Journal of Biological Macromolecules, 148, 292-301. https://doi.org/10.1016/j.ijbiomac.2020.01.120
[2]  David, S., Femi, B., Gbenga, A. and Adeleke, B.S. (2017) Purification and Characterization of α-Amylase from Bacillus subtilis Isolated from Cassava Processing Sites. Journal of Bioremediation & Biodegradation, 8, 2-7. https://doi.org/10.4172/2155-6199.1000417
[3]  Wu, X.R., Wang, Y.X., Tong, B.D., Chen, X.H. and Jian, H. (2018) Purification and Biochemical Characterization of a Thermostable and Acid-Stable Alpha-Amylase from Bacillus licheniformis B4-423. International Journal of Biological Macromolecules: Structure, Function and Interactions, 109, 329-337. https://doi.org/10.1016/j.ijbiomac.2017.12.004
[4]  Aehle, W. (2007) Enzymes in Industry: Production and Applications. Wiley-VCH/ Verlag GmbH and Co., Weinheim. https://doi.org/10.1002/9783527617098
[5]  Arikan, B. (2008) Highly Thermostable, Thermophilic, Alkaline, SDS and Chelator Resistant Amylase from a Thermophilic Bacillus sp. Isolate A3-15. Bioresource Technology, 99, 3071-3076. https://doi.org/10.1016/j.biortech.2007.06.019
[6]  Rajagopalan, G. and Krishnan, C. (2008) Alpha-Amylase Production from Catabolite Derepressed Bacillus subtilis KCC103 Utilizing Sugarcane Bagasse Hydrolysate. Bioresource Technology, 99, 3044-3050. https://doi.org/10.1016/j.biortech.2007.06.001
[7]  Padma, S. and Pallavi, K. (2016) Isolation and Characterization of Amylase Producing Bacillus spp. from Selected Soil Sample. International Journal of Research in BioSciences, 5, 24-29.
[8]  Kaur, P.S., Kaur, S., Kaur, H. and Sondhi, S. (2019) Purification and Characterization of a Amylase from Bacillus licheniformis MTCC 1483 Having Applications in Textile Industry. IJEAM, 54, 363-368.
[9]  Souza, P.M.D. and Pérola, O.M. (2010) Application of Microbial α-Amylase in Industry—A Review. Brazilian Journal of Microbiology, 41, 850-861. https://doi.org/10.1590/S1517-83822010000400004
[10]  Nielsen, J.E. and Borchert, T.V. (2000) Protein Engineering of Bacterial Alpha-Amylases. Biochimica Et Biophysica Acta, 1543, 253-274. https://doi.org/10.1016/S0167-4838(00)00240-5
[11]  Couto, S.R. and Sanroman, M.A. (2006) Application of Solid-State Fermentation to Food Industry—A Review. Journal of Food Engineering, 76, 291-302. https://doi.org/10.1016/j.jfoodeng.2005.05.022
[12]  Jaiswal, N. and Prakash, O. (1998) Immobilization of Soybean a-Amylase on Gelatin and its Application as a Detergent Additive. Asian Journal of Biochemistry, 6, 337-346. https://doi.org/10.3923/ajb.2011.337.346
[13]  Saideh, A.A., Abdolhamid, B.D. and Namaki, S. (2016) Characterization of a Thermostable, CaCl2-Activated and Raw-Starch Hydrolyzing Alpha-Amylase from Bacillus licheniformis AT70: Production under Solid State Fermentation by Utilizing Agricultural Wastes. Journal of Molecular Catalysis B: Enzymatic, 132, 98-106. https://doi.org/10.1016/j.molcatb.2016.07.002
[14]  Ahmed, S.A., Abdella, M.A., El-Sherbiny, G.M., Ibrahim, A.M. and Atalla, S.M. (2019) Application of One-Factor-at-a-Time and Statistical Designs to Enhance α-Amylase Production by a Newly Isolate Bacillus subtilis Strain-MK1. Biocatalysis and Agricultural Biotechnology, 22, Article ID: 101397. https://doi.org/10.1016/j.bcab.2019.101397
[15]  Raul, D., Biswas, T., Mukhopadhyay, S., Das, S.K. and Gupta, S. (2014) Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation. Biochemistry Research International, 2014, Article ID: 586141. https://doi.org/10.1155/2014/568141
[16]  Li, Y.J. and Hu, Q.P. (2020) Effect of Bacillus subtilis QM3 on β-Amylase Isoenzyme in Early Germination of Wheat Seed. South Asian Journal of Research in Microbiology, 6, 24-32. https://doi.org/10.9734/sajrm/2020/v6i230146

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