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The Efficacy of Rapid Diagnostic Test in the Diagnosis of Malaria among Adults as Compared to Microscopy in a Hospital in Imo State, South Eastern Nigeria

DOI: 10.4236/oalib.1106528, PP. 1-11

Subject Areas: Epidemiology

Keywords: Malaria, Efficacy, Rapid Diagnostic Test, Microscopy

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Malaria has been noted to be one of the most prevalent diseases affecting mankind. Diagnosis is based on clinical assessment using microscopy and recently by rapid diagnostic tests (RDTs). The objective of this study was to ascertain the efficacy of RDT to microscopy in the identification of malaria parasite among adults attending a hospital in Owerri, Imo State. A cross-sectional study was carried out using 110 consenting outpatients of a hospital in Imo state, Nigeria. Results of the study showed that more respondents were females 63 (57.3%). Microscopy was positive in 97.3% (107) patients, and RDT was positive in 100% (32) of the adult, while 2.7% of those negative by microscopy were also negative with RDT. There was no significant difference when the accuracy of both tests was compared (P = 0.261, χ2 = 1.262). The RDT used had a sensitivity of 29.9%, a specificity of 100%. The positive predictive value was 100% and the negative predictive value 3.85%. The microscopy performance was extremely high compared to RDT in the diagnosis of malaria in adults. Microscopy is recommended, if available to be done for all patients suspected of having malaria with negative RDT in other not to miss out people with the disease as it remains the gold standard for malaria diagnosis.

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Dozie, U. W. , Ekeh, N. A. , Iwuoha, G. N. , Nwaokoro, C. J. , Asuzu, N. E. and Dozie, I. N. S. (2020). The Efficacy of Rapid Diagnostic Test in the Diagnosis of Malaria among Adults as Compared to Microscopy in a Hospital in Imo State, South Eastern Nigeria. Open Access Library Journal, 7, e6528. doi:


[1]  Center for Disease Control and Prevention (2010) About Malaria.
[2]  Reyburn, H., Mbakilwa, H., Mwangi, R., Mwerinde, O., Olomi, R., Drakeley, C. and Whitty, C.J. (2007) Rapid Diagnostic Tests Compared with Malaria Microscopy for Guiding Outpatient Treatment of Febrile Illness in Tanzania: Randomised Trial. British Medical Journal, 334, 403.
[3]  Wongsrichanalai, C., Barcus, M.J., Muth, S., Sutamihardja, A. and Wernsdorfer, W.H. (2007) A Review of Malaria Diagnostic Tools: Microscopy and Rapid Diagnostic Test (RDT). American Journal of Tropical Medical Hygiene, 77, 119-127.
[4]  Harvey, S.A., Jennings, L., Chinyama, M., Masaninga, F., Mulholland, K. and Bell, D.R. (2008) Improving Community Health Worker Use of Malaria Rapid Diagnostic Tests in Zambia: Package Instructions, Job Aid and Job Aid-plus-Training. Malaria Journal, 7, 1475-2875.
[5]  Swarthout, T.D., Counihan, H., Senga, R.K.K. and van den Broek, I. (2007) Paracheck-Pf Accuracy and Recently Treated Plasmodium falciparum Infections: Is There a Risk of Over-Diagnosis? Malaria Journal, 6, 58.
[6]  Abeku, T.A., Kristan, M., Jones, C., Beard, J., Mueller, D.H., Okia, M., Rapuoda, B., Greenwood, B. and Cox, J. (2008) Determinants of the Accuracy of Rapid Diagnostic Tests in Malaria Case Management: Evidence from Low and Moderate Transmission Settings in the East African Highlands. Malaria Journal, 7, 202.
[7]  Hopkins, H., Bebell, L., Kambale, W., Dokomajilar, C., Rosenthal, P.J. and Dorsey, G. (2008) Rapid Diagnostic Tests for Malaria at Sites of Varying Transmission Intensity in Uganda. Journal of Infectious Disease, 197, 510-518. James, S.P. and Tate, P. (2004) Exo-Erythrocytic Schizogony in Plasmodium gallinaceum Brumpt. Parasitology, 30, 128-138.
[8]  Harris, I., Sharrock, W.W., Bain, L.M., Gray, K.A., Bobogare, A., Boaz, L., Lilley, K., Krause, D. and Vallely, A. (2010) A Large Proportion of Asymptomatic Plasmodium Infections with Low and Sub-Microscopic Parasite Densities in the Low Transmission Setting of Temotu Province, Solomon Islands: Challenges for Malaria Diagnostics in an Elimination Setting. Malaria Journal, 9, 254.
[9]  Laurent, A., Schellenberg, J., Shirima, K., Ketende, S.C., Alonso, P.L., Mshinda, H., Tanner, M. and Schellenberg, D. (2010) Performance of HRP-2 Based Rapid Diagnostic Test for Malaria and Its Variation with Age in an Area of Intense Malaria Transmission in Southern Tanzania. Malaria Journal, 9, 294.
[10]  Mtove, G., Nadjm, B., Amos, B., Hendriksen, I.C.E., Muro, F. and Reyburn, H. (2011) Use of an HRP2-Based Rapid Diagnostic Test to Guide Treatment of Children Admitted to Hospital in a Malaria-Endemic Area of North-East Tanzania. Tropical Medicine and International Health, 16, 545-550.
[11]  Kalu, M.K., Nwogo, A.O., Onyemachi, F. and Otuchristi, G.A. (2012) Comparative Study of the Prevalence of Malaria in Aba and Umuahia Urban Areas of Abia State, Nigeria. Research Journal of Parasitology, 7, 17-24.
[12]  Ibekwe, A.C., Okonko, I.O., Onunkwo, A.I., Ogun, A.A. and Udeze, A.O. (2009) Comparative Prevalence Level of Plasmodium in Freshmen (First Year Students) of Nnamdi Azikwe University in Akwa, Southern-Eastern, Nigeria. Malaysian Journal of Microbiology, 5, 51-54.
[13]  Olatunji, M.K., Olugbenga, A.M., Yetunde, A.O. and Tolulope, O.O. (2017) Comparative Prevalence of Plasmodium falciparum Malaria in Patients Attending Okelele Health Center, Okelele, Ilorin, Nigeria. India Journal of Health Sciences and Biomedical Research, 10, 57-62.
[14]  Adeyemo, F.O., Okpala, P.U., Oyana, E.N. and Imoukhuede, M.O. (2014) Malaria Infection amongst Students of the University of Benin, Edo State, Nigeria. International Journal of Recent Scientific Research, 5, 1529-1532.
[15]  Ruquayyah, M.H., Nock, I.H., Ndams, S.I. and George, B.D.J. (2017) Investigating the Prevalence and Intensity of Plasmodium falciparum Infection and Two Methods of Malaria Diagnosis in North-Western Nigeria. International Journal of Tropical Disease and Health, 23, 1-8.
[16]  Enitan, S.S., Emmanuel, I., Adejumo, E.N., Olayimika, K.A., Gideon, T.F. and Adedoyin, O.A. (2019) Assessment of Rapid Diagnostic Test and Microscopy in the Detection of Plasmodium falciparum Malaria Infection among Young Adults in South-Western Nigeria. South Asian Journal of Parasitology, 2, 1-21.
[17]  Adesina, O.O. (2013) Subclinical Malaria Infection among University of Maiduguri Students: Prevalence and Parasite Density. BOMJ, 10, 6-10.
[18]  Oyeyemi, O.T., Ogunlade, A.F. and Oyewole, I.O. (2015) Comparative Assessment of Microscopy and Rapid Diagnostic Test (RDT) as Malaria Diagnostic Tools Comparative Assessment of Microscopy and Rapid Diagnostic Test (RDT) as Malaria Diagnostic Tools.
[19]  Mahende, C., Billy, N., John, L., Tai-Soon, Y., Paminus, L., Martha, L., Bruno, M. and Zul, P. (2016) Performance of Rapid Diagnostic Test, Blood-Film Microscopy and PCR for the Diagnosis of Malaria Infection among Febrile Children from Korogwe District, Tanzania. Malaria Journal, 15, 391.
[20]  Ojurongbe, O., Adegbosin, O.O., Taiwo, S.S., Alli, O.A., Olowe, O.A., Ojurongbe, T.A., Oloyede, S.B. and Oluwaseyi, A.A. (2013) Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria. Malaria Research and Treatment, 2013, Article ID: 308069.
[21]  Garba, B.I., Muhammad, A.S., Musa, A., Edem, B., Yusuf, I., Bello, N.K., Adeniji, A.O. and Kolawole, T. (2006) Diagnosis of Malaria: A Comparison between Microscopy and Rapid Diagnostic Test among Under-Five Children at Gusau, Nigeria. Sub-Saharan African Journal of Medicine, 3, 96-101.
[22]  Endeshaw, T., Gebre, T., Ngondi, J., Patricia, M.G., Estifanos, B., Ejigsemahu, S.Y., Ayele, B., Yohannes, G., Teferi, T., Messele, A., Zerihun, M., Genet, A., Mosher, A.W., Emerson, P.M. and Richards, F.O. (2008) Evaluation of Light Microscopy and Rapid Diagnostic Test for the Detection of Malaria under Operational Field Conditions: A Household Survey in Ethiopia. Malaria Journal, 7, Article 118.
[23]  Marx, A., Pewsner, D., Egger, M., Nüesch, R., Bucher, H.C., Genton, B., Hatz, C. and Jüni, P. (2005) Meta-Analysis: Accuracy of Rapid Tests for Malaria in Travellers Returning from Endemic Areas. Annals of Internal Medicine, 142, 836-846.
[24]  Jelinek, T., Grobusch, M.P. and Nothdurft, H.D. (2000) Use of Dipstick Tests for the Rapid Diagnosis of Malaria in Nonimmune Travellers. Journal of Travel Medicine, 7, 175-179.
[25]  WHO (2011) Malaria Rapid Diagnostic Test Performance: Summary Results of WHO Malaria RDT Product Testing: Rounds 1-3 (2008-2011). World Health Organization, Geneva.
[26]  Ilesanmi, R.I., Oluwasogo, A.O., Oluwasegun, T.A., Ayodele, O.I., Hyacinth, E. and Abimbola, O.A. (2017) Comparative Assessment of Malaria Rapid Diagnostic Tests (RDT) in Ibadan, Nigeria. Malaria Journal, 8, 17.
[27]  Awortu, J.Z., Uko, E.K., Buseri, F.I. and Awortu, J.T. (2007) Field Evaluation of SD Bioline Rapid Malaria Diagnostic Test Kit among Asymptomatic Malaria-Infected Children in Port Harcourt, Nigeria. Research Journal of Parasitology, 2, 39-44.
[28]  Enebeli, U.U., Amadi, A.N., Okechukwu, K.I., Oparaocha, E.T., Nwoke, E.A., Oti, N.N., Ibe, S.N.O., Chukwuocha, U.M., Nwufo, C.R., Amadi, C.O. and Esomonu, I. (2019) A Comparison of the Rapid Diagnostic Test and Microscopy for Malaria Diagnosis in Children under Five in Abia State, Nigeria. International Journal of Research and Scientific Innovation, 6, 199-202.
[29]  Murungi, M., Fulton, T., Reyes, R., Matte, M., Ntaro, M., Mulogo, E. and Buyce, R. (2017) Improving the Specificity of Plasmodium falciparum Malaria Diagnosis in High-Transmission Settings with a Two-Step Rapid Diagnostic Test and Microscopy Algorithm. Journal of Clinical Microbiology, 55, 1540-1549.
[30]  Albertini, A., Lee, E., Coulibaly, S.O., Sleshi, M., Faye, B. and Mationg, M.L. (2012) Malaria Rapid Diagnostic Test Transport and Storage Conditions in Burkina Faso, Senegal, Ethiopia and the Philippines. Malaria Journal, 11, 406.


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