All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

ViewsDownloads

Potential Application of Hibiscus sabdariffa L. (Malvaceae) Aqueous Extract for Assessment of Viability of Protoscolices from Hydatid Cysts

DOI: 10.4236/oalib.1106398, PP. 1-8

Subject Areas: Veterinary Medicine, Parasitology

Keywords: Hibiscus sabdariffa, Calyces, Viability, Protoscolices, Hydatid Cysts

Full-Text   Cite this paper   Add to My Lib

Abstract

Cystic echinococcosis (hydatidosis) is a serious zoonotic parasitic disease of global public health and economic importance. It is caused by infection with hydatid cysts, the larva of a tapeworm of canines, particularly dogs. The canine hosts become infected with the adult parasite when they ingest with viscera fertile hydatid cysts containing protoscolices (PSCs). Generally, the mere presence of PSCs in a hydatid cyst has been considered an indication of infectivity to the host. There is no tangible measure, however, to assess the degree of this infectivity apart from feeding experimentally to the host (dogs) a known number of PSCs and counting at necropsy the number of adult worms that develop in the intestine. This approach, however, though technically feasible, has several limitations not least among which is the potential error in finding such very small worms in the intestine and the health hazard from exposure to infective eggs released by patent worms. To avoid such risks, a number of commercial stains have been used in in vitro tests to assess the viability of PSCs (defined as the capacity of being alive), a property closely linked to infectivity. Hibiscus sabdariffa is a flowering plant widely cultivated in Sudan. Aqueous extracts from plant calyces have characteristic brilliant red coloration due to the presence of anthocyanins, an important group of water-soluble plant pigments. The present study examines the potential application of aqueous extract of plant calyces for assessment of viability of PSCs taking uptake/exclusion of plant pigment as criteria. It is proposed that the application of extract can be used effectively as an objectively quantifiable low-cost assay for assessment of viability of PSCs from hydatid cysts. Performance of extract is comparable to eosin.

Cite this paper

Elowni, E. E. , Ahmad, M. F. , Abdelnabi, G. H. and Badawi, R. M. (2020). Potential Application of Hibiscus sabdariffa L. (Malvaceae) Aqueous Extract for Assessment of Viability of Protoscolices from Hydatid Cysts . Open Access Library Journal, 7, e6398. doi: http://dx.doi.org/10.4236/oalib.1106398.

References

[1]  WHO/OIE (2002) Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Organization for Animal Health and World Health Organization.
[2]  Miman, O., Aycan, O.M., Aydin, C. and Atambay, M. (2010) What Should Be the Concentration of Eosin to Qualification of Ideal Staining for Viability Determination on Hydatid Cyst? Türk Hijyen ve Deneysel Biyoloji Dergisi, 67, 21-26.
[3]  Esfandiari, B. and Youssef, M.R. (2010) Comparison of Eosin and Trypan Blue Staining in Viability of Hydatid Cyst Protoscolices. Global Veterinaria, 4, 456-458.
[4]  Elowni, E.E., Ahmad, M.F., Abdelnabi, G.H. and Badawi, R.M. (2019) Validation of the Methylene Blue Test for Assessment of Viability of Protoscolices from Hydatid Cysts. Open Veterinary Journal, 9, 172-176. https://doi.org/10.4314/ovj.v9i2.13
[5]  Abou-Arab, A.A., Abu-Salem, F.M. and Abou-Arab, E.A. (2011) Physico-Chemical Properties of Natural Pigments (Anthocyanin) Extracted from Roselle Calyces (Hibiscus sabdariffa). Journal of American Science, 7, 445-456.
[6]  Shruthi, V.H., Ramachandra, C.T., Udaykumar, N., Sharanagouda, H., Nagaraj, N. and Kurubar, R.A. (2016) Roselle (Hibiscus sabdariffa L) as a Source of Natural Color: A Review. Plant Archives, 16, 515-522.
[7]  Horbowicz, M., Kosson, R., Grzesiuk, A. and Dbski, H. (2008) Anthocyanins of Fruits and Vegetables—Their Occurrence, Analysis and Role in Human Nutrition. Vegetable Crops Research Bulletin, 68, 5-22. https://doi.org/10.2478/v10032-008-0001-8
[8]  Eltayeib, A.A. and Hamade, H. (2014) Phytochemical and Chemical Composition of Water Extract of Hibiscus sabdariffa (Red Karkade Calyces) in North Kordofan State Sudan. International Journal of Advanced Research in Chemical Science, 1, 10-13.
[9]  Moazeni, M. and Alipour-Chaharmahali, M.R. (2011) Echinococcus granulosus: In Vitro Effectiveness of Warm Water on Protoscolices. Experimental Parasitology, 127, 14-17. https://doi.org/10.1016/j.exppara.2010.06.021
[10]  Yones, D.A., Taher, G.A. and Ibraheim, Z.Z. (2011) In Vitro Effects of Some Herbs Used in Egyptian Traditional Medicine on Viability of PSCs of Hydatid Cysts. Korean Journal of Parasitology, 49, 255-263. https://doi.org/10.3347/kjp.2011.49.3.255
[11]  Mahmoudvand, H., Dezaki, E.S., Kheirandish, F., Ezatpour, B., Jahanbakhsh, S. and Harandi, M.F. (2014) Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts. Korean Journal of Parasitology, 52, 653-659. https://doi.org/10.3347/kjp.2014.52.6.653
[12]  Mokhtaria, K. and Ammar, S.S.M. (2019) Frozen Hydatid Cysts Can Replace Incineration and Sterilize Cysts. Open Veterinary Journal, 9, 1-4. https://doi.org/10.4314/ovj.v9i1.1
[13]  Lumsden, R.D. and Specian, R. (1980) The Morphology, Histology and Fine Structure of the Adult Stage of the Cyclophyllidean Tapeworm Hymenolepis diminuta. In: Arai, H.P., Ed., Biology of the Tapeworm Hymenolepis diminuta, Academic Press, Cambridge, 157-280. https://doi.org/10.1016/B978-0-12-058980-7.50008-7
[14]  Podesta, R.B. (1980) Concepts of Membrane Biology in Hymenolepis diminuta. In: Arai, H.P., Ed., Biology of the Tapeworm Hymenolepis diminuta, Academic Press, Cambridge, 505-549. https://doi.org/10.1016/B978-0-12-058980-7.50014-2
[15]  Dalton, J.P., Skelly, P. and Halton, D.W. (2004) Role of the Tegument and Gut in Nutrient Uptake by Parasitic Platyhelminthes. Canadian Journal of Zoology, 82, 211-232. https://doi.org/10.1139/z03-213
[16]  Jeffs, S.A., Hurd, H., Allen, J.T. and Arme, C. (1978) Kinetics of Molecular Transfer across the Tegument of Protoscolices and Hydatid Cysts of Echinococcus granulosus and the Relevance of These Studies to Drug Targeting. In: Geerts, S., Kumar, V. aand Brandt, J., Eds., Helminth Zoonoses, Springer, Dordrecht, 37-43. https://doi.org/10.1007/978-94-009-3341-5_6
[17]  Elmahdi, I.E., Ali, Q.M., Magzoub, M.M., Ibrahim, A.M., Saad, M.B. and Romig, T. (2004) Cystic Echinococcosis of Livestock and Humans in Central Sudan. Annals of Tropical Medicine and Parasitology, 98, 473-479. https://doi.org/10.1179/000349804225003578
[18]  Sahar, A.M. and Abdelgadir, A.E. (2011) Study on Hydatid Cyst Infection in Slaughterhouses in Khartoum State, Sudan. Archives of Applied Science Research, 3, 18-23.
[19]  Ibrahim, K., Romig, T., Peter, K. and Omer, R.A. (2011) A Molecular Survey on Cystic Echinococcosis in Sinnar Area, Blue Nile State (Sudan). Chinese Medical Journal, 124, 2829-2833.
[20]  Ismail, N.B.O., Elfadil, A.A. and Ahmed, E.G.M. (2016) Epidemiological Study on Bovine Hydatidosis in North Kordofan State, Sudan. International Journal of Veterinary Science, 5, 74-78.
[21]  Mohammed, A. and El Malik, K.H. (1999) The Epidemiology of Cystic Echinococcosis in Nyala, Southern Darfur State, Sudan. The Sudan Journal of Veterinary Research, 16, 49-53.
[22]  Omer, R.A., Dinkel, A., Romig, T., Mackenstedt, U., Elnahas, A.A., Aradaib, I.E., et al. (2010) A Molecular Survey of Cystic Echinococcosis in Sudan. Veterinary Parasitology, 169, 340-346. https://doi.org/10.1016/j.vetpar.2010.01.004
[23]  Abdel-Moemin, A.R. (2016) Effect of Roselle Calyces Concentrate with Other Ingredients on the Physiochemical and Sensory Properties of Cupcakes. Journal of Food Processing and Technology, 7, 603. https://doi.org/10.4172/2157-7110.1000603
[24]  Aishah, B., Nursabrina, M., Noriham, A., Norizzah, A.R. and Shahrimi, H.M. (2013) Anthocyanins from Hibiscus sabdariffa, Melastoma malabathricum and Ipomoea batatas and Its Color Properties. International Food Research Journal, 20, 827-834.

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

微信:OALib Journal