In this paper we used BSA Langmuir monolayer as templates to study the effect of solute concentration on regulated biomineralization of calcium carbonate. During the crystallization process, it is found that the morphology of the calcium carbonate can be strongly affected by the solute concentration in presence of BSA Langmuir monolayer. X-ray Diffraction (XRD) results show that the crystal of calcium carbonate formed is all the calcite phase with a higher orientation. SEM images show that the morphology of calcite is time-dependent at different solute concentrations. The effect of solute concentration on morphology was discussed and the interaction of particles with bovine serum albumin Langmuir monolayers was proposed.
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