Cholera is an infectious diarrheal disease, highly contagious transmitted by a gram-negative bacteria, Vibrio cholerae. The infectious strain is divided into two biotypes (El Tor and Classical) based on their phenotypic characteristics and three serotypes (Inaba, Ogawa, Hikojima) according to the antiserum agglutination test. The Hikojima serotypes form is very rarely found during an epidemic. To date, several studies have shown that the CTX phage ？ which is a vibriophage, confers on the bacterium its infectious nature. Through its lysogenic cycle, it converts the bacterial host from a non-pathogenic strain to a pathogenic strain, thereby providing virulence genes to the bacterial host. In 2011, several cases of cholera were reported worldwide, and in the Democratic Republic of Congo, the first cases of cholera were identified in 1994 following the displacement of Rwandan refugees in the town of Goma, bordering Rwanda. Since then, cholera has become endemic in this region. In 2017, several cases of cholera have been reported in several provinces of the Democratic Republic of Congo including Kinshasa. Hygienic measures must be taken into account to fight effectively against the disease. Strengthening the capacity of laboratory staff, the establishment of rapid molecular diagnostic tools is necessary for proper care of people living in areas likely to develop the cholera epidemic.
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Diabeno, V. and Kamangu, E. (2019). Vibrio cholerae: General Knowledge and Epidemiological Aspects. Open Access Library Journal, 6, e5571. doi: http://dx.doi.org/10.4236/oalib.1105571.
Sedas, V.T. (2007) Influence of Environmental Factors on the Presence of Vibrio cholera in the Marine Environment: A Climate Link. The Journal of Infection in Developing Countries, 1, 224-241. https://doi.org/10.3855/jidc.359
Kanungo, S., Tsuzuki, A., Deen, J.L., Lopez, A.L., Rajendran, K., Manna, B., Sur, D., Kim, D.R., Gupta, V.K., Ochiai, R.L., Ali, M., von, S.L., Bhattacharya, S.K. and Clemens, J.D. (2010) Use of Verbal Autopsy to Determine Mortality Patterns in an Urban Slum in Kolkata, India. Bulletin of the World Health Organization, 88, 667-674. https://doi.org/10.2471/BLT.09.073742
Mason, P.R. (2009) Zimbabwe Experiences the Worst Epidemic of Cholera in Africa. The Journal of Infection in Developing Countries, 3, 148-151. https://doi.org/10.3855/jidc.62
Das, S., Choudhry, S., Saha, R., Ramachandran, V.G., Kaur, K. and Sarkar, B.L. (2011) Emergence of Multiple Drug Resistance Vibrio cholera O1 in East Delhi. The Journal of Infection in Developing Countries, 5, 294-298. https://doi.org/10.3855/jidc.1251
Tobin D’angelo, M., Smith, A.R., Bulens, S.N., et al. (2008) Severe Diarrhea Caused by Cholera Toxin Producing Vibrio Cholera Serogroup O75 Infections Acquired in the South Eastern United States. Clinical Infectious Diseases, 47, 1035-1040. https://doi.org/10.1086/591973
Dalsgaard, A., Serichantalergs, O., Forslund, A., Lin, W., Mekelanos, J., Mintz, E., Shimada, T. and Well, J.G. (2001) Clinical and Environmental Isolates of Vibrio Cholera Serogroup O141 Carry the CTX Phage and the Genes Encoding the Toxin Coregulated Pili. Journal of Clinical Microbiology, 39, 4036-4092. https://doi.org/10.1128/JCM.39.11.4086-4092.2001
Jongsik, C., Christopher, J.G., Nur, A.H., Je, H.L., Seon, Y.C., Bradd, J.H., Elisa, T., Yoon, S.J., Dong, W.K., Jae, H.L., Thomas, S.B., David, C.B., Jean, F.C., Chris, J.D., Cliff, S.H., Christine, A.M., Olga, C., Linda, M., Elizabeth, S., Ronald, A.W., Anwar, H., Balakrish, G.N. and Rita, R.C. (2009) Comparative Genomics Reveals Mechanism for Short-Term and Long-Term Clonal Transitions in Pandemic Vibrio cholerae. Proceedings of the National Academy of Sciences of the United States of America, 106, 15442-15447. https://doi.org/10.1073/pnas.0907787106
Nair, G.B., Faruque, S.M., Bhuiyan, N.A., Kamruzzaaman, M., Siddique, A.K. and Sack, D.A. (2002) New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrheal in Bangladesh. Journal of Clinical Microbiology, 40, 3296-3299. https://doi.org/10.1128/JCM.40.9.3296-3299.2002
Faruque, S.M., Tam, V.C., Chowdhury, N., Diraphat, P., Dziejman, M., Heidelberg, J.F., Clemens, J.D., Mekelanos, J.J. and Nair, G.B. (2007) Genomic Analysis of the Mozambique Strain of Vibrio cholerae O1 Reveals the Origin of El tor Strains Carrying Classical CTX Prophage. Proceedings of the National Academy of Sciences of the United States of America, 104, 5151-1516. https://doi.org/10.1073/pnas.0700365104
Nair, G.B., Qadri, F., Holmgren, J., et al. (2006) Cholera Due to Altered El Tors Trains ofVibrio cholerae O1 in Bangladesh. Journal of Clinical Microbiology, 44, 4211-4213. https://doi.org/10.1128/JCM.01304-06
Bhabatosh, D., Julien, B. and Fran？ois, X.B. (2011) Molecular Mechanism of Acquisition of the Cholera Toxingenes. Indian Journal of Medical Research, 133, 195-200.
Faruque, S.M., Islam, M.J., Ahmad, Q.S., Faruque, A.S., Sack, D.A., Nair, G.B., Mekalanos, J.J. (2005) Self-Limiting Nature of Seasonal Cholera Epidemics: Role of Host-Mediated Amplification of Phage. Proceedings of the National Academy of Sciences of the United States of America, 102, 6119-6124. https://doi.org/10.1073/pnas.0502069102
Kimsey, H.H., Nair, G.B., Ghosh, A. and Waldor, M.K. (1998) Diverse CTXphis and Evolution of New Pathogenic Vibrio cholerae. The Lancet, 352, 457-458. https://doi.org/10.1016/S0140-6736(05)79193-5
Val, M.E., El Kennedy, S.P., Karoui, M., Bonné, L., Chevalier, F. and Barre, F.X. (2008) FtsK-Dependent Dimer Resolution on Multiple Chromosomes in the Pathogen Vibrio cholera. PLoS Genet, 4, e1000201. https://doi.org/10.1371/journal.pgen.1000201
Lencer, W.I. andTsai, B. (2003) The Intracellular Voyage of Cholera Toxin: Going Retro. Trends in Biochemical Sciences, 28, 639-645. https://doi.org/10.1016/j.tibs.2003.10.002
Davis, B.M. and Waldor, M.K. (2002) Mobile Genetic Elements and Bacterial Pathogenesis. American Society for Microbiology Press, Washington DC, 1040-1059. https://doi.org/10.1128/9781555817954.ch45
Ochman, H., Lawrence, J.G. and Groisman, E.A. (2000) Lateral Gene Transfer and the Nature of Bacterial Innovation. Nature, 405, 299-304. https://doi.org/10.1038/35012500
Boyd, E.F., Heilpern, A.J. and Waldor, M.K. (2000) Molecular Analyses of a Putative CTXphi Precursor and Evidence for Independent Acquisition Distinct CTX (w) s by Toxigenic Vibrio cholerae. Journal of Bacteriology, 182, 5530-5538. https://doi.org/10.1128/JB.182.19.5530-5538.2000
Boyd, F. (2008) Filamentous Phages of Vibrio cholerae. In: Faruque, S. and Nair, G.B., Eds., Vibriocholerae: Genomics and Molecular Biology, Horizon Scientific Press, UK, 49-66.
Jensen, M.A., Faruque, S.M., Mekalanos, J.J. and Levin, B.R. (2006) Modeling the Role of Bacteriophage in the Control of Cholera Outbreaks. Proceedings of the National Academy of Sciences of the United States of America, 103, 4652-4657. https://doi.org/10.1073/pnas.0600166103
Shah, M.F. and Mekalanos, J.J. (2012) Phage-Bacterial Interactions in the Evolution of Toxygenic Vibrocholerae. Virulence, 3, 7. https://doi.org/10.4161/viru.22351
Faruque, S.M. and Mekalanos, J.J. (2003) Pathogenicity Islands and Phages in Vibrio cholerae Evolution. Trends in Microbiology, 11, 505-510. https://doi.org/10.1016/j.tim.2003.09.003
Karaolis, D.K., Johnson, J.A., Bailey, C.C., Boedeker, E.C., Kaper, J.B. and Reeves, P.R.A. (1998) Vibrio cholerae Pathogenicity Island Associated with Epidemic and Pandemic Strains. Proceedings of the National Academy of Sciences of the United States of America, 95, 3134-3139. https://doi.org/10.1073/pnas.95.6.3134
Davis, B.M., Kimsey, H.H., Chang, W. and Waldor, M.K. (1999) The Vibrio cholerae O139 Calcutta Bacteriophage CTXphi Is Infec-tious and Encodes a Novel Repressor. Journal of Bacteriology, 181, 6779-6787.
Bhattacharya, T., Chatterjee, S., Maiti, D., Bhadra, R.K., Takeda, Y., Nair, G.B., et al. (2006) Molecular Analysis of the rstR and orfU Genes of the CTX Prophages Integrated in the Small Chromosomes of Environmental Vibrio cholera non-O1, non-O139 Strains. Environmental Microbiology, 8, 526-634. https://doi.org/10.1111/j.1462-2920.2005.00932.x
Ministry of Public Health, General Secretariat (2012) Epidemiological Situation of Cholera in the Democratic Republic of Congo in 2011. Cellule de SUM du plan stratégique d’élimination du Choléra en RDC, 25, 27-28, 31, 33-34.
Ministry of Public Health, General Secretariat (2017) Epidemiological Situation of Cholera in the Democratic Republic of Congo, Week 48. National Program for the Elimination of Cholera and Diarrheal Disease Control in the DRC. 4-5.
Ministry of Public Health, General Secretariat (2013) Multisectoral Strategic Plan for the Elimination of Cholera in the Democratic Republic of Congo 2013-2017. 21 p.
