全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

Separation and Purification of Ursolic Acid from Cynomorium songaricum Exacts with Macroporous Resins

DOI: 10.4236/oalib.1104475, PP. 1-12

Subject Areas: Biotechnology

Keywords: Ursolic Acid, Macroporous Resins, Cynomorium songaricum Rupr

Full-Text   Cite this paper   Add to My Lib

Abstract

Enrichment and purification of ursolic acid from Cynomorium songaricum exacts were studied using five macroporous resins. The static tests indicated that D101 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. To optimize the separation process, dynamic adsorption and desorption tests were carried out. The optimal adsorption parameters were initial concentrations in sample solution of 3.8 mg/mL, pH of 6.0, sample loading amount of 5 BV, flow rate of 2 BV/h, and temperature of 25?C. The optimal desorption parameters were 70% ethanol 5 BV, then 80% ethanol 10 BV, and flow rate of 2 BV/h. After one run treatment with AB-8 resin, the content of total flavonoids in the product increased from 9.83% to 67.8%, and the recovery yield was 84.02%. The results showed that AB-8 resin revealed a good ability to enrichment total flavonoids from Cynomorium songaricum, and the method can be referenced for the enrichment of total flavonoids from other materials.

Cite this paper

Zhang, X. , Hou, W. and Zheng, X. (2018). Separation and Purification of Ursolic Acid from Cynomorium songaricum Exacts with Macroporous Resins. Open Access Library Journal, 5, e4475. doi: http://dx.doi.org/10.4236/oalib.1104475.

References

[1]  Liu, G.D., Chen, G.L., Li, W. and Li, C.X. (2013) Genetic and Phytochemical Diversities of Cynomorium songaricum Rupr. in Northwest China Indicated by ISSR Markers and HPLC-Fingerprinting. Biochemical Systematics and Ecology, 48, 34-41. https://doi.org/10.1016/j.bse.2012.12.004
[2]  Ma, L.J., Chen, G.L., Jin, S.W. and Wang, C.X. (2006) The Anti-Aging Effect and the Chemical Consttuents of Cynomorium songaricum Rupr. Acta Horticulturae, 765, 23-30.
[3]  Jin, S.W., Chen, G.L., Du, J.J.M., Wang, L.H., Ren, X. and An, T.Y. (2014) Antioxidant Properties and Principal Phenolic Compositions of Cynomorium songaricum Rupr. International Journal of Food Properties, 17, 13-25.
https://doi.org/10.1080/10942912.2011.587623
[4]  Ma, C.M., Nakamura, N., Miyashiro, H., Hattori, M. and Shimotohno, K. (1999) Inhibitory Effects of Constituents from Cynomorium songari-cum and Related Triterpene Derivatives on HIV-1 Protease. Chemical and Pharmaceutical Bulletin, 47, 141-145.
[5]  Ma, L.J., Chen, G.L., Nie, L.S. and Ai, M. (2009) Effect of Cynomorium songaricum Polysaccharide on Telomere Length in Blood and Brain of D-Galactose-Induced Senescence Mice. China Journal of Chinese Materia Medica, 34, 1257-1260.
[6]  Zhang, X.R., Jia, Z.P., Li, M.X., Wang, J., Yin, Q., Luo, J.D. and Liu, H.Y. (2008) Study on the Effect of Part III from Cynomorium songaricum on Immu-nosuppressive Mice Induced by Cyclophosphamide. Journal of Chinese Medicinal Materials, 31, 407-409.
[7]  Tao, J., Tu, P., Xu, W. and Chen, D. (1999) Studies on Chemical Constituents and Pharmacological Effects of the Stem of Cynomorium songaricum Rupr. Journal of Chinese Medicinal Materials, 24, 292-294.
[8]  Zheng, L.L., Wang, D., Li, Y.Y., Peng, H.Y., Yuan, M.Y. and Gao, F. (2014) Ultrasound-Assisted Extraction of Total Flavonoids from Aconitum gymnandrum. Pharmacognosy Magazine, 10, 141-146.
https://doi.org/10.4103/0973-1296.127364
[9]  Cai, F., Xiao, C., Chen, Q., Ouyang, C., Zhang, R., Wu, J. and Liu, C. (2014) Orthogonal Test Design for Optimizing the Extraction of Total Flavonoids from Flos Pueraria. African Journal of Pharmacy and Pharmacology, 8, 1-8.
https://doi.org/10.5897/AJPP2013.3939
[10]  Srinivas, K., King, J.W., Monrad, J.K., Howard, L.R. and Zhang, D. (2011) Pressurized Solvent Extraction of Flavonoids from Grape Pomace Utilizing Organic Acid Additives. Italian Journal of Food Science, 23, 90-105.
[11]  Bi, W., Tian, M. and Row, K.H. (2013) Evaluation of Alcohol-Based Deep Eutectic Solvent in Extraction and Determination of Flavonoids with Response Surface Methodology Optimization. Journal of Chromatography A, 1285, 22-30.
https://doi.org/10.1016/j.chroma.2013.02.041
[12]  Yin, H., Zhang, S., Long, L., Yin, H., Tian, X., Luo, X. and He, S. (2013) The Separation of Flavonoids from Pongamia pinnata Using Combination Columns in High-Speed Counter-Current Chromatography with a Three-Phase Solvent System. Journal of Chromatography A, 1315, 80-85.
https://doi.org/10.1016/j.chroma.2013.09.010
[13]  Dmitrienko, S.G., Stepanova, A.V., Kudrinskaya, V.A. and Apyari, V.V. (2012) Specifics of Separation of Flavonoids by Reverse Phase High Performance Liquid Chromatography on the Luna 5u C18(2) Column. Moscow University Chemistry Bulletin, 67, 254-258.
[14]  Goncalves, M.S., Vieira, I.J.C., Oliveira, R.R. and Braz-Filho, R. (2011) Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis (Apocynaceae). Molecules, 16, 7480-7487.
https://doi.org/10.3390/molecules16097480
[15]  Jiang, Z.G., Du, Q.Z. and Sheng, L.Y. (2009) Separation and Purification of Lentinan by Preparative High Speed Counter Current Chromatography. Chinese Journal of Analytical Chemistry, 37, 412-416.
[16]  Shi, S., Zhang, Y., Huang, K., Liu, S. and Zhao, Y. (2008) Application of Preparative High-Speed Counter-Current Chromatography for Separation and Purification of Lignans from Taraxacum mongolicum. Food Chemistry, 108, 402-406.
https://doi.org/10.1016/j.foodchem.2007.10.069
[17]  Yin, L., Xu, Y., Qi, Y., Han, X., Xu, L., Peng, J. and Sun, C.K. (2010) A Green and Efficient Protocol for Industrial-Scale Preparation of Dioscin from Dioscorea nipponica Makino by Two-Step Macroporous Resin Column Chromatography. Chemical Engineering Journal, 165, 281-289.
https://doi.org/10.1016/j.cej.2010.09.045
[18]  Sun, P.C., Liu, Y., Yi, Y.T., Li, H.J., Fan, P. and Xia, C.H. (2015) Preliminary Enrichment and Separation of Chlorogenic Acid from Helianthus tuberosus L. Leaves Extract by Macroporous Resins. Food Chem-istry, 168, 55-62.
https://doi.org/10.1016/j.foodchem.2014.07.038
[19]  Li, C.L., Shi, X.L., Men, Y., Yang, L. and Yang, A.M. (2014) Purification of Astragalus Polysaccharide with Macroporous Resins. Applied Mechanics and Materials, 618, 326-329.
https://doi.org/10.4028/www.scientific.net/AMM.618.326
[20]  Wang, C., Chao, Z., Wu, X., Sun, W. and Ito, Y. (2014) En-richment and Purification of Pedunculoside and Syringin from the Barks of Ilex rotunda with Macroporous Resins. Journal of Liquid Chromatography & Related Technologies, 37, 572-587.
https://doi.org/10.1080/10826076.2012.749499
[21]  Lv, X.H., Kuang, P.Q., Yuan, Q.P., Liang, H. and Zheng, G.Q. (2014) Preparative Separation of Steviol Glycosides from Stevia rebaudiana Bertoni by Macroporous Resin and Preparative HPLC. Acta Chromatographica, 26, 123-135.
https://doi.org/10.1556/AChrom.26.2014.1.10
[22]  Kim, E.J., Lee, H.J., Kim, H.J., Nam, H.S., Lee, M.K., Kim, H.Y. and Kim, H.Y. (2005) Comparison of Colorimetric Methods for the Determination of Flavonoid in Propolis Extract Products. Korean Journal of Food Science and Technology, 37, 918-921.
[23]  Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. (2002) Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10, 178-182.
[24]  Afsar, M.Z., Hoque, S. and Osman, K.T. (2012) A Comparison of the Langmuir, Freundlich and Temkin Equations to Describe Phosphate Sorption Characteristics of Some Representative Soils of Bangladesh. International Journal of Soil Science, 7, 91-99.
https://doi.org/10.3923/ijss.2012.91.99
[25]  Tellan, A.C. and Owalude, S.O. (2007) Some Langmuir and Freundlich Parameters of Adsorption Studies of Chlorpheniramine Maleate. Research Journal of Applied Sciences, 2, 875-878.
[26]  Xiao, F. and Pignatello, J.J. (2014) Effect of Adsorption Nonlinearity on the pH-Adsorption Profile of Ionizable Organic Compounds. Langmuir, 30, 1994-2001.
https://doi.org/10.1021/la403859u
[27]  Zhang, Y., Li, S.F., Wu, X.W. and Zhao, X. (2007) Macroporous Resin Adsorption for Purification of Flavonoids in Houttuynia cordata Thunb. Chinese Journal of Chemical Engineering, 15, 872-876.
https://doi.org/10.1016/S1004-9541(08)60017-8
[28]  Langmuir, I. (1918) Adsorption of Gases on Plain Surface of Glass, Mica and Platinum. Journal of the American Chemical Society, 40, 1361-1403.
https://doi.org/10.1021/ja02242a004
[29]  Freundlich, H. (1907) Kolloidfallung und Adsorption. Angewandte Chemie, 20, 749-750.
[30]  Ferraro, V., Cruz, I.B., Jorge, R.F., Pintado, M.E. and Castro, P.M. (2013) Effects of Physical Parameters onto Adsorption of the Borderline Amino Acids Glycine, Lysine, Taurine, and Tryptophan upon Amberlite XAD16 Resin. Journal of Chemical & Engineering Data, 58, 707-717.
https://doi.org/10.1021/je301206r
[31]  Zhao, Z.Y., Dong, L.L., Wu, Y.L. and Lin, F. (2011) Preliminary Separation and Purification of Rutin and Quercetin from Euonymus alatus (Thunb.) Siebold Extracts by Macroporous Resins. Food and Bioproducts Processing, 89, 266-272.
https://doi.org/10.1016/j.fbp.2010.11.001
[32]  Zhao, H., Wang, J., Jia, J., Liu, J., Ling, X.Q. and Lu, D.Q. (2010) Enrichment and Purification of Total Chlorogenic Acids from Tobacco Waste Extract with Macroporous Resins. Separation Science and Technology, 45, 794-800.
https://doi.org/10.1080/01496390903566713

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133

WeChat 1538708413