The removal of pesticides in the environment mainly
depends on natural degradation, especially on microbial degradation.
Biodegradation has many advantages, such as
complete degradation, no secondary pollution, quick effect and wide spectrum.
Based on the single-factor experiments and Box-Benhnken design, the effect of four factors on the degradation of
chlorpyrifos by P. stutzeri ZH-1 was investigated. The four factors,
including temperature (℃), oscillator speed
(rpm), inoculum concentration (%) and pH, and their interactions on the
degradation of chlorpyrifos were studied through the use of response surface
analysis. The optimal conditions of chlorpyrifos-degrading were
as follows: Temperature 36.7℃, oscillator speed 130.00 rpm, inoculum concentration 7%, pH 7. Under these
conditions, the
degradation rate of chlorpyrifos was 96.48%. Moreover, P. stutzeri ZH-1 could be used efficiently for remediation of contaminated
soils.
Cite this paper
He, F. , Zhang, M. , Zhang, L. and Hu, Q. (2018). Response Surface Methodology for the Optimization of Chlorpyrifos-Degrading Conditions by Pseudomonas stutzeri ZH-1. Open Access Library Journal, 5, e4405. doi: http://dx.doi.org/10.4236/oalib.1104405.
Cycon’, M., Wójcik, M. and Piotrowskaseget, Z. (2009) Biodegradation of the Organophosphorus Insecticide Diazinon by Serratia sp. and Pseudomonas sp. and Their Use in Bioremediation of Contaminated Soil. Chemosphere, 76, 494-501. https://doi.org/10.1016/j.chemosphere.2009.03.023
Li, X., Jiang, J., Gu, L., Ali, S.W., He, J. and Li, S. (2008) Diversity of Chlorpyrifos-Degrading Bacteria Isolated from Chlorpyrifos-Contaminated Samples. International Biodeterioration & Biodegradation, 62, 331-335. https://doi.org/10.1016/j.ibiod.2008.03.001
Singh, B.K., Walker, A., Morgan, J.A. and Wright, D.J. (2003) Effects of Soil pH on the Biodegradation of Chlorpyrifos and Isolation of a Chlorpyrifos-Degrading Bacterium. Applied & Environmental Microbiology, 69, 5198-206. https://doi.org/10.1128/AEM.69.9.5198-5206.2003
Singh, B.K., Walker, A. and Wright, D.J. (2006) Bioremedial Potential of Fenamiphos and Chlorpyrifos Degrading Isolates: Influence of Different Environmental Conditions. Soil Biology & Biochemistry, 38, 2682-2693. https://doi.org/10.1016/j.soilbio.2006.04.019
Mallick, K., Bharati, K., Banerji, A., Shakil, N.A. and Sethunathan, N. (1999) Bacterial Degradation of Chlorpyrifos in Pure Cultures and in Soil. Bulletin of Environmental Contamination & Toxicology, 62, 48-54. https://doi.org/10.1007/s001289900840
Singh, B.K., Walker, A., Morgan, J.A.W. and Wright, D.J. (2004) Biodegradation of chlorpyrifos by Enterobacter strain B-14 and Its Use in Bioremediation of Contaminated Soils. Applied & Environmental Microbiology, 70, 4855-4863. https://doi.org/10.1128/AEM.70.8.4855-4863.2004
Holden, P.A. and Firestone, M.K. (1997) Soil Microorganisms in Soil Cleanup: How Can We Improve Our Understanding? Journal of Environmental Quality, 26, 32-40. https://doi.org/10.2134/jeq1997.00472425002600010006x
Chen, K., Liu, X.M., Li, R., Liu, Y., Hu, H., Li, S.P., et al. (2011) Isolation of a Buprofezin Co-Metabolizing Strain of Pseudomonas sp. DFS35-4 and Identification of the Buprofezin Transformation Pathway. Biodegradation, 22, 1135-1142. https://doi.org/10.1007/s10532-011-9469-x
Yu, T. (2015) Biological Characteristics of Organophosphate Degrading Bacteria and Enzymology Properties Analysis of Organophosphate Degradation Enzyme.
Aboamer, A. (2011) Biodegradation of Diazinon by Serratia marcescens DI101 and Its Use in Bioremediation of Contaminated Environment. Journal of Microbiology & Biotechnology, 21, 71. https://doi.org/10.4014/jmb.1007.07024
Sen, R. and Swaminathan, T. (2004) Response Surface Modeling and Optimization to Elucidate and Analyze The Effects of Inoculum Age and Size on Surfactin Production. Biochemical Engineering Journal, 21, 141-148. https://doi.org/10.1016/j.bej.2004.06.006
Gangadharan, D., Sivaramakrishnan, S., Nampoothiri, K.M., Sukumaran, R.K. and Pandey, A. (2008) Response Surface Methodology for the Optimization of Alpha Amylase Production by Bacillus Amyloliquefaciens. Bioresource Technology, 99, 4597-4602. https://doi.org/10.1016/j.biortech.2007.07.028
Mahiudddin, M., Fakhruddin, A.N.M., Chowdhury, M.A.Z., Rahman, M.A. and Alam, M.K. (2014) Degradation of the Organophosphorus Insecticide Diazinon by Soil Bacterial Isolate. International Journal of Biotechnology, 2014, 12-23.
Sikora, L.J., Kaufman, D. and Hornog, L.C. (1990) Enzyme Activity in Soils Showing Degradation of Organophosphosphate Insecticides. Biology and Fertility of Soils, 9, 14-18. https://doi.org/10.1007/BF00335855
Racke, K.D. and Robbins, S.T. (1991) Factors Affecting the Degradation of 3,5,6-trichloro-2-pyridinol in Soil. ACS Symposium Series, 459, 93-107. https://doi.org/10.1021/bk-1991-0459.ch007
Yang, L., Zhao, Y.H., Zhang, B.X. and Zhang, X. (2005) Isolation and Characterization of a Chlorpyrifos Degrading Bacteria and Its Bioremediation Application in the Soil. Acta Microbiologica Sinica, 45, 905-909.
Zhang, D.Y., Tan, X.Q., Luo, X.W., Zhu, C.H., Luo, Y.H., Ming-Yuan, H.E., et al. (2005) Isolation of Photosynthetic Bacteria hp-1 with Degradation of Organic-Phosphorus Insecticides and Studies on Its Biodegradation Ability and Capacity of Increasing Growth. Life Science Research, 9, 247-253.
