All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

ViewsDownloads

The Investigation of the Apoptose Structural Effects and Mechanism in Leukemic Cells of Sirt1 Inhibitor Sirtinol

DOI: 10.4236/oalib.1104268, PP. 1-6

Subject Areas: Hematology

Keywords: SIRT1, Sirtinol, Leukemia Cell

Full-Text   Cite this paper   Add to My Lib

Abstract

Human SIRT1 is an enzyme that deacetylates the p53 tumor suppressor protein. It has been suggested to modulate p53-dependent functions including DNA damage-induced cell death. Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. In this study, we investigated the relationship between sirtinols and apoptosis mechanism at leukemic cells. For this, we applied sirtinol to K-562 (chronic myeloid leukemia) and Jurkat (acute T-cell leukemia) cell lines at different dilutions in cell culture conditions. And Cdna isolated patient RNA samples, after that Caspase3, Bax, Bcl2 expression performed with used qRT-PCR technique and cells were stained by Annexin V method. According to the results of research, we identified that Sirtinol dilutions were increased in both K-562 and Jurkat, while the number of living cells was decreasing, the number of dead cells increased at 50 μM dilution. Sirtinol, an HDAC inhibitor in the direction of these results, has been observed to be a drug that can be effective in the treatment of CML as well as T-ALL.

Cite this paper

Akin, D. F. , Mumcuoglu, M. , Ozkan, D. T. , Akar, N. and Kurekci, A. E. (2018). The Investigation of the Apoptose Structural Effects and Mechanism in Leukemic Cells of Sirt1 Inhibitor Sirtinol. Open Access Library Journal, 5, e4268. doi: http://dx.doi.org/10.4236/oalib.1104268.

References

[1]  Yuan, H., Su, L. and Chen, W.Y. (2013) The Emerging and Diverse Roles of Sirtuins in Cancer: A Clinical Perspective. Onco Targets and Therapy, 6, 1399-1416.
[2]  Michishita, E., Park, J.Y., Burneskis, J.M., Barrett, J.C. and Horikawa, I. (2005) Evolutionarily Conserved and Nonconserved Cellular Localizations and Functions of Human SIRT Proteins. Molecular Biology of the Cell, 16, 4623-4635.
https://doi.org/10.1091/mbc.E05-01-0033
[3]  Liu, T., Liu, P.Y. and Marshall, G.M. (2009) The Critical Role of the Class III Histone Deacetylase SIRT1 in Cancer. Cancer Research, 69, 1702-1705.
https://doi.org/10.1158/0008-5472.CAN-08-3365
[4]  Fraga, M.F. and Esteller, M. (2007) Epigenetics and Aging: The Targets and the Marks. Trends in Genetics, 23, 413-418.
https://doi.org/10.1016/j.tig.2007.05.008
[5]  Lim, C.S. (2007) Human SIRT1: A Protein Biomarker for Tumorigenesis? Cell Bio logy International, 31, 636-637.
https://doi.org/10.1016/j.cellbi.2006.11.003
[6]  Kozako, T., Aikawa A., Shoji, T., Fujimoto, T., et al. (2012) High Expression of the Longevity Gene Product SIRT1 and Apoptosis Induction by Sirtinol in Adult T-Cell Leukemia Cells. T. International Journal of Cancer, 131, 2044-2055.
https://doi.org/10.1002/ijc.27481
[7]  Mumcuoglu, M., Akin, D.F., Ezer, u. and Akar, N. (2015) Ankaferd Blood Stopper Induces Apoptosis and Regulates PAR1 and EPCR Expression in Human Leukemia Cells. Egyptian Journal of Medical Human Genetics, 83, 19-27.
https://doi.org/10.1016/j.ejmhg.2014.10.001

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

微信:OALib Journal