Human SIRT1 is an enzyme that deacetylates the p53 tumor
suppressor protein. It has been suggested to modulate p53-dependent functions including
DNA damage-induced cell death. Sirtuins are nicotinamide adenine dinucleotide dependent
class III histone deacetylase proteins that play a crucial role in several cellular
processes, including DNA repair, apoptosis, and lifespan. In this study, we investigated
the relationship between sirtinols and apoptosis mechanism at leukemic cells. For
this, we applied sirtinol to K-562 (chronic myeloid leukemia) and Jurkat (acute
T-cell leukemia) cell lines at different dilutions in cell culture conditions. And
Cdna isolated patient RNA samples, after that Caspase3, Bax, Bcl2 expression performed
with used qRT-PCR technique and cells were stained by Annexin V method. According to the results of research, we identified that Sirtinol dilutions were increased in both K-562 and Jurkat, while the number
of living cells was decreasing,
the number of dead cells increased at 50 μM dilution. Sirtinol, an HDAC inhibitor in the direction of these
results, has been observed to be a drug that can be effective in the treatment of
CML as well as T-ALL.
Cite this paper
Akin, D. F. , Mumcuoglu, M. , Ozkan, D. T. , Akar, N. and Kurekci, A. E. (2018). The Investigation of the Apoptose Structural Effects and Mechanism in Leukemic Cells of Sirt1 Inhibitor Sirtinol. Open Access Library Journal, 5, e4268. doi: http://dx.doi.org/10.4236/oalib.1104268.
Michishita, E., Park, J.Y., Burneskis, J.M., Barrett, J.C. and Horikawa, I. (2005) Evolutionarily Conserved and Nonconserved Cellular Localizations and Functions of Human SIRT Proteins. Molecular Biology of the Cell, 16, 4623-4635. https://doi.org/10.1091/mbc.E05-01-0033
Kozako, T., Aikawa A., Shoji, T., Fujimoto, T., et al. (2012) High Expression of the Longevity Gene Product SIRT1 and Apoptosis Induction by Sirtinol in Adult T-Cell Leukemia Cells. T. International Journal of Cancer, 131, 2044-2055. https://doi.org/10.1002/ijc.27481
Mumcuoglu, M., Akin, D.F., Ezer, u. and Akar, N. (2015) Ankaferd Blood Stopper Induces Apoptosis and Regulates PAR1 and EPCR Expression in Human Leukemia Cells. Egyptian Journal of Medical Human Genetics, 83, 19-27.