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Activities of Lactate Dehydrogenase, Methemoglobin, Carboxyhemoglobin and Sulfhemoglobin Concentrations in the Blood of Albino Rats Exposed to Cashew Seed Fume

DOI: 10.4236/oalib.1102284, PP. 1-6

Subject Areas: Toxicology

Keywords: Cashew Seed Fume, Methemoglobin, Sulfhemoglobin, Carboxyhemoglobin, Albino Wistar Rats

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Smoke produced in cashew processing factories during roasting of the seed contains toxic chemicals which have adverse health effects on workers exposed to it. This study investigated the effect of cashew seed fume on lactate dehydrogenase (LDH) activity, methemoglobin (Hi), carboxyhemoglobin (HbCO) and sulfhemoglobin (SHb) in the blood of rats exposed to cashew seed fumes (CSF), via the dynamic exposure system. Eighteen (18) male albino rats were used; they were divided into three groups of six animals each as: control, rats exposed for two (2) hours and rats exposed four (4) hours for five days. The results showed that serum level of LDH in rats exposed to CSF was significantly (P < 0.05) increased compared with the control rats. The %SHb and %HbCO in the blood of rats exposed to CSF were significantly (P < 0.05) increased compared with the control rats. However there were no significant (P > 0.05) differences in the % methemoglobin level of rats in all groups. This study revealed that exposure to CSF may have adverse effects on the lungs and may also result in system poisoning. This should be a cause of concern for industrial regulatory bodies who should regulate the safety of cashew factory workers.

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Tope, M. T. , Adedotun, I. D. , Dorcas, A. T. , Bode, O. M. , Otaru, O. W. and Esther, A. T. (2016). Activities of Lactate Dehydrogenase, Methemoglobin, Carboxyhemoglobin and Sulfhemoglobin Concentrations in the Blood of Albino Rats Exposed to Cashew Seed Fume. Open Access Library Journal, 3, e2284. doi:


[1]  Morton, J.F. (1987) Cashew Apple. Fruits of Warm Climates, 11, 239-240.
[2]  De Souza, C.P., Mendes, N.M., Janotti-Passos, L.K. and Pereira, J.P. (1992) The Use of the Shell of Cashew Nut, Anacardium occidentale, as an Alternative Molluscacide. Revista do Instituto de Medicina Tropical de Sao Paulo, 34, 459-466.
[3]  Dare, S.S., Hamman, W.O., Musa, S., Goji, A.D.T., Oyewale, A.A., Abba, S. and Ezekiel, I. (2011) Effects of Aqueous Extract of Anacardium occidentale (Cashew) Leaf on Pregnancy Outcome of Wistar Rats. International Journal of Animal and Veterinary Advances, 3, 77-82.
[4]  ITDG (2001) The Cashew Nut Processing. Intermediate Technology, 1, 1-8.
[5]  Alexander, H.T. (2008) A Nutty Chemical. Chemical and Engineering News, 86, 26-27.
[6]  Master, A., Santosh, K.D. and Sachidananda, P. (2006) Eco-Consciousness for Poisonous and Injurious Plants among Urban Dwellers of Bhubaneswar, Orissa. Journal of Human Ecology, 19, 239-248.
[7]  Ravil, K. and Patil, M.B. (2009) Evaluation of Anacardium occidentale Gum as Gelling Agent in Acelofenac Gel. Journal of PharmTech Research, 3, 695-704.
[8]  John, B. (2006) 21st Century Children’s Encyclopedia. Academic (India) Publishers, New Delhi, 13-14.
[9]  Silver, S.D. (1946) Constant Flow Gassing Chambers: Principles Influencing Design and Operation. Journal of Laboratory and Clinical Medicine, 31, 1153-1161.
[10]  Barbara, J.B., Imelda, B., Micheal, A.L. and Lewis, M.S. (2012) Laboratory Methods Used in the Investigation of the Hemolytic Anaemia. In: Dacie and Lewis Practical Haematology, 11th Edition, Church Hill Living Stone, 240-242.
[11]  Deutsche Gesellschaft fur Klinische Chemie (DGKC) (1970) Determination of Lactate Dehydrogenase Activity Using UV Method. Journal of Clinical Chemistry and Clinical Biochemistry, 8, 658.
[12]  Vasudevan, D.M., Vaidynath, K. and Sreekumari, S. (2013) Biochemistry for Medical Students. 7th Edition, Jaypee Brothers Medical Publisher Ltd., New Delhi, 346-360.
[13]  Flexman, A.M., Del Vicario, G. and Schwarz, S.K. (2007) Dark Green Blood in the Operating Theatre. The Lancet, 369, 9577-9578.
[14]  Goldfrank, L., Flomenbaum, N., Lewin, N., Howland, M.A., Hoffman, R. and Nelson, L. (2002) Carbon Monoxide. In: Goldfranks Toxicologic Emergencies, 7th Edition, McGraw-Hill, New York, 1689-1704.
[15]  Berg, J. (2011) Chapter 10: Regulatory Strategies. Berg, T. and Biochemistry, S., Eds., 7th Edition, WH. Freeman and Company Limited, New York.
[16]  Bateman, D.N. (2003) Carbon Monoxide. Medicine, 31, 41-42.
[17]  Lott, J.A. and Nemensanszky, E. (1987) Lactate Dehydrogenase. In: Lott, J.A., Wolf, P.L. and Sawhney, A.K., Eds., Clinical Enzymology: A Case-Oriented Approach, Field and Rich/Year Book, New York, 213-244.
[18]  Glick, J.H. (1969) Serum Lactate Dehydrogenase Isoenzyme and Total Lactate Dehydrogenase Values in Health and Disease, and Clinical Evaluation of These Tests by Means of Discriminant Analysis. American Journal of Clinical Pathology, 52, 320-328.
[19]  Warren, E.C., Wacker, M.D., David, D., Ulmer, M.D., Bert, L. and Vallee, M.D. (1956) Metalloeneymes and Myocardial Infarction-Malic and Lactic Dehydrogenase Activities and Zinc Concentrations in Serum. The New England Journal of Medicine, 255, 449-456.
[20]  Shahjahan, M., Jainu, S.M. and Devi, C.S.S. (2004) Effect of Solanum trilobatum against Carbon Tetrachloride Induced Hepatic Damage in Albino Rats. Indian Journal of Medical Research, 120, 194-198.


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