Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.

prior to obtain a standardized dried extract from v. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. an analytical method using hplc-pda for lupeol determination in v. ferruginea intermediate products was developed using a c8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 ml min-1, oven temperature at 23-25 oc, sample injection volume at 30 μl and detection at 210 nm. the method presented linearity from 10 to 160 μg ml-1, accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of v. ferruginea.