%0 Journal Article
%T Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.
%A Oliveira
%A Ezequiane Machado Silva
%A Freitas
%A Sabrine Lemes
%A Martins
%A Frederico Severino
%A Couto
%A Ren¨º Oliveira
%A Pinto
%A Mariana Viana
%A Paula
%A Jos¨¦ Realino
%A Concei£¿£¿o
%A Edemilson Cardoso
%A Bara
%A Maria Teresa Freitas
%J Qu¨ªmica Nova
%D 2012
%I Sociedade Brasileira de Qu¨ªmica
%R 10.1590/S0100-40422012000500032
%X prior to obtain a standardized dried extract from v. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. an analytical method using hplc-pda for lupeol determination in v. ferruginea intermediate products was developed using a c8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 ml min-1, oven temperature at 23-25 oc, sample injection volume at 30 ¦Ìl and detection at 210 nm. the method presented linearity from 10 to 160 ¦Ìg ml-1, accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of v. ferruginea.
%K quality control
%K triterpene
%K standardized dried extract.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-40422012000500032&lng=en&nrm=iso&tlng=en