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Cloning and expression analysis of rubredoxin from cold-treated banana leaves

Keywords: rubredoxin, expression, banana, stress response.

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Abstract:

a banana (musa aaa, cavendish subgroup cv. brazil) cdna encoding a putative rubredoxin-like protein (mard1) was obtained from total rna isolated from cold-treated banana leaves using rapid amplification of cdna ends (race) technique. mard1 cdna contained 597 nucleotides encoding 198 amino acids in the open reading frame. mard1 protein showed 56% amino acid identity with that of pyrococcus furiosus rubredoxin (p24297). a chloroplast transit peptide and a transmembrane region were detected at the n-terminus and the c-terminus, respectively, of the deduced amino acid sequence of mard1 gene. southern blotting revealed the occurrence of at least two copies of mard1 in the banana genome. real time quantitative rt-pcr analysis revealed that the expression of mard1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. cold and salt stresses induced higher levels of mard1 transcript accumulation in leaves. this finding indicated a role of mard1 in the response to these abiotic stresses.

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