%0 Journal Article
%T Cloning and expression analysis of rubredoxin from cold-treated banana leaves
%A Feng1
%A RJ
%A Lu
%A LF
%A Yuan
%A KH
%A Cheng
%A P
%A Zhang
%A LL
%A Qi
%A JF
%A Ren
%A Y
%A Xu
%A XL
%A Zhang
%A XB
%A Zhou
%A LY
%A Zhang
%A YD
%J Phyton (Buenos Aires)
%D 2010
%I Scientific Electronic Library Online
%X a banana (musa aaa, cavendish subgroup cv. brazil) cdna encoding a putative rubredoxin-like protein (mard1) was obtained from total rna isolated from cold-treated banana leaves using rapid amplification of cdna ends (race) technique. mard1 cdna contained 597 nucleotides encoding 198 amino acids in the open reading frame. mard1 protein showed 56% amino acid identity with that of pyrococcus furiosus rubredoxin (p24297). a chloroplast transit peptide and a transmembrane region were detected at the n-terminus and the c-terminus, respectively, of the deduced amino acid sequence of mard1 gene. southern blotting revealed the occurrence of at least two copies of mard1 in the banana genome. real time quantitative rt-pcr analysis revealed that the expression of mard1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. cold and salt stresses induced higher levels of mard1 transcript accumulation in leaves. this finding indicated a role of mard1 in the response to these abiotic stresses.
%K rubredoxin
%K expression
%K banana
%K stress response.
%U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S1851-56572010000200008&lng=en&nrm=iso&tlng=en