Partial chemical and functional characterization of milk whey products obtained by different processes

whey protein samples (s-1 to s-5) were tested in vivo and in vitro for nutritional properties and selected bioactivities. weanling male wistar rats fed modified ain-93g (12 g protein.100 g-1) diets for 21 days were used the in vivo studies. the nutritional parameters did not differ among the protein diets tested. erythrocyte glutathione content was considered high and was higher for s-3, but liver glutathione was the same for all dietary groups. for s-3, cytokine secretion (il-10 and tnf-α) by human peripheral blood mononuclear cells (in rpmi-1640 medium) was higher in the absence of antigen than in the presence of bcg antigen. interleukin-4 secretion was repressed in all treatments. the ic50, whey protein concentration required to inhibit 50% of the melanoma cell proliferation, was 2.68 mg.ml-1 of culture medium for the s-3 sample and 3.66 mg.ml-1 for the s-2 sample. based on these results, it was concluded that s-3 (whey protein concentrate enriched with tgf-β and lactoferrin) produced better nutritional and immunological responses than the other products tested.