Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

the present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name chitinolyticbacter meiyuanensis sybc-h1 is proposed. a sequential statistical methodology comprising of plackett-burman and response surface methodology (rsm) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. as a result, maximum chitinase activity of 5.17 u/ml was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. the triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.