%0 Journal Article
%T Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1
%A Hao
%A Zhikui
%A Cai
%A Yujie
%A Liao
%A Xiangru
%A Zhang
%A Xiaoli
%A Fang
%A Zhiyou
%A Zhang
%A Dabing
%J Brazilian Journal of Microbiology
%D 2012
%I Sociedade Brasileira de Microbiologia
%R 10.1590/S1517-83822012000100019
%X the present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name chitinolyticbacter meiyuanensis sybc-h1 is proposed. a sequential statistical methodology comprising of plackett-burman and response surface methodology (rsm) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. as a result, maximum chitinase activity of 5.17 u/ml was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. the triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
%K chitinase
%K medium optimization
%K response surface method
%K inulin
%K chitinolyticbacter meiyuanensis sybc-h1.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1517-83822012000100019&lng=en&nrm=iso&tlng=en