Proteomic analysis of whey from bovine colostrum and mature milk

the aim of this study was to standardize a methodology to obtain two-dimensional (2d) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (maldi tof/tof ms). a total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.