%0 Journal Article %T Proteomic analysis of whey from bovine colostrum and mature milk %A Golinelli %A Luciana Pacheco %A Conte-Junior %A Carlos Adam %A Paschoalin %A Vania Margaret Flosi %A Silva %A Joab Trajano %J Brazilian Archives of Biology and Technology %D 2011 %I Tecpar %R 10.1590/S1516-89132011000400016 %X the aim of this study was to standardize a methodology to obtain two-dimensional (2d) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (maldi tof/tof ms). a total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time. %K bovine whey proteins %K colostrum proteins %K low-abundance proteins %K two-dimensional electrophoresis %K mass spectrometry %K matrix-assisted laser desorption [ionization tandem time-of-flight mass spectrometry (maldi tof/tof ms)]. %U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132011000400016&lng=en&nrm=iso&tlng=en