A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain

total rna from lipopolysaccharide (lps)-stimulated rat macrophages used to treat protoplasts from an aspergillus nidulans strain originated the rt2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in wistar rats. the protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. the screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male swiss mice. biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). total sugar quantification revealed 32% glycosylation of the protein fraction. amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. sds-page revealed that the protein sample is pure and its molecular weight is about 40kda. intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. the isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.