%0 Journal Article
%T A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
%A Queiroz
%A J. C. F.
%A Teixeira
%A C. F. P.
%A Lebrun
%A I.
%A Spencer
%A P. J.
%A Zucchi
%A T. M. A. D.
%J Journal of Venomous Animals and Toxins including Tropical Diseases
%D 2007
%I Centro de Estudos de Venenos e Animais Pe?onhentos - CEVAP, Universidade Estadual Paulista - UNESP
%R 10.1590/S1678-91992007000200010
%X total rna from lipopolysaccharide (lps)-stimulated rat macrophages used to treat protoplasts from an aspergillus nidulans strain originated the rt2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in wistar rats. the protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. the screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male swiss mice. biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). total sugar quantification revealed 32% glycosylation of the protein fraction. amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. sds-page revealed that the protein sample is pure and its molecular weight is about 40kda. intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. the isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
%K aspergillus nidulans
%K protein purification and characterization
%K glycoprotein
%K anti-inflammatory activity.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1678-91992007000200010&lng=en&nrm=iso&tlng=en