A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction

a modification of the cscl gradient centrifugation method for dna phage purification is presented. it avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. the quality of the dna obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.