%0 Journal Article %T A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction %A Santelli %A Roberto V. %A Navarro-Cattapan %A Luci Deise %J Genetics and Molecular Biology %D 2000 %I Sociedade Brasileira de Gen¨¦tica %R 10.1590/S1415-47572000000100011 %X a modification of the cscl gradient centrifugation method for dna phage purification is presented. it avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. the quality of the dna obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc. %U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572000000100011&lng=en&nrm=iso&tlng=en