Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts

β-lactoglobulin (blg), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. a shrna targeting blg mrna with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shrna interference in goat-fetal fibroblast cells. apart from high efficiency in the knockdown of blg expression in these cells, lentivector-mediated rnai manifested stable integration into the goat genome itself. consequently, an in vitro model for goat blg-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.