%0 Journal Article
%T Stable silencing of ¦Â-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
%A Zhang
%A Shumin
%A Xiong
%A Kai
%A Xie
%A Zhourui
%A Nan
%A Wenting
%A Liu
%A Honglin
%A Chen
%A Jie
%J Genetics and Molecular Biology
%D 2012
%I Sociedade Brasileira de Gen¨¦tica
%R 10.1590/S1415-47572012005000042
%X ¦Â-lactoglobulin (blg), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. a shrna targeting blg mrna with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shrna interference in goat-fetal fibroblast cells. apart from high efficiency in the knockdown of blg expression in these cells, lentivector-mediated rnai manifested stable integration into the goat genome itself. consequently, an in vitro model for goat blg-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.
%K rnai
%K shrna
%K lentivirus
%K blg
%K goat.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572012000400020&lng=en&nrm=iso&tlng=en