Evaluación del método de conservación en papel de filtro en dos cepas de Bacillus subtilis Cohn mediante la actividad antagónica frente a Rhizoctonia solani Kühn

antagonistic activity of two bacillus subtilis strains preserved in filter paper disks at 4°c for a period of three years was evaluated. strains bs-21 and bs-42, isolated from cuban soil and antagonists to rhizoctonia solani rs-10 were choiced. five colonies from bs-42 and one from bs-21, randomly selected were growth in nutrient agar. the antagonism was compared by dual culture method in pda after 96 h at 30°c. viability of both strains was 108 cfu/ml. selected colonies kept purity, morphological characteristics and positive response to gram stain compared with b. subtilis atcc 6633. all selected colonies inhibited mycelial growth of r. solani rs-10, but not the reference strain. strain bs-21 had the lower inhibition (78%) while bs-421 and bs-422 had the higher percent (98%). variance analysis showed significant differences in the percentage of inhibition exhibited by tested colonies.