Background: Dietary antioxidants are widely used to reduce oxidative stress and enhance immune system function. Smoking is a known generator of reactive oxygen species, leading to oxidative stress, which can adversely affect general health and skin health. Methods: We completed a randomized, placebo-controlled double-blind clinical trial to investigate the effects of Solarplast？, an enzyme treated spinach supplement, on oxidative stress, immune function, and skin health, in a sample of male and female smokers and non-smokers. Fifty-nine participants were randomly assigned to 60-day daily ingestion of Solarplast？ (100 mg) or placebo to determine the effects on white blood cell counts and distribution, protein oxidation, lipid peroxidation, and ex vivo cytokine concentration (TNF-α, IL-6, IL-10, and IL-1β) with and without lipopolysaccharide (LPS) stimulation. Normalized cytokine levels were determined by dividing the LPS-stimulated levels by the non-stimulated levels. Skin health was assessed using a Pear 3D Plus Skin Analysis system and questionnaires determined subjects’ perceived health. Change scores from baseline were used for comparing treatments and results between smokers and non-smokers. Results: Solarplast？ was well-tolerated, with no adverse events noted. Treatment did not result in a statistically significant reduction for oxidative stress markers of Advanced Oxidation Protein Products (AOPP) (13% reduction) or malondialdehyde (MDA) (15% reduction) from baseline in smokers. Treatment reduced normalized ex vivo TNF-α levels (58%) from baseline, but this finding did not reach statistical significance due to the high variability across subjects. Solarplast？ significantly reduced exvivo normalized IL-1β more than 30% at both day 30 (p = 0.008) and day 60 (p = 0.026) as compared to placebo, with a more pronounced effect in the non-smokers (43% and 46% reduction at day 30 and day 60, respectively) as compared to the smokers (31% and 25% reduction at day 30 and day 60, respectively). Values for measurements of skin appearance resulted in higher moisture levels at day 60 for the Solarplast？ group compared to placebo, regardless of smoking status. Conclusion: This exploratory study suggests that Solarplast？, a novel spinach supplement appears safe and well-tolerated for human consumption and can reduce the normalized cytokine response ex
References
[1]
Halliwell, B. (1984) Oxygen Radicals: A Commonsense Look at Their Nature and Medical Importance. Medical Biology, 62, 71-77. https://pubmed.ncbi.nlm.nih.gov/6088908/
[2]
Hussain, T., Tan, B., Yin, Y., Blachier, F., Tossou, M.C.B. and Rahu, N. (2016) Oxidative Stress and Inflammation: What Polyphenols Can Do for Us? OxidativeMedicineandCellularLongevity, 2016, e7432797. https://doi.org/10.1155/2016/7432797
[3]
Pryor, W.A. and Stone, K. (1993) Oxidants in Cigarette Smoke Radicals, Hydrogen Peroxide, Peroxynitrate, and Peroxynitritea. AnnalsoftheNewYorkAcademyofSciences, 686, 12-27. https://doi.org/10.1111/j.1749-6632.1993.tb39148.x
[4]
Alberg, A.J. (2002) The Influence of Cigarette Smoking on Circulating Concentrations of Antioxidant Micronutrients. Toxicology, 180, 121-137. https://doi.org/10.1016/s0300-483x(02)00386-4
[5]
van der Vaart, H. (2004) Acute Effects of Cigarette Smoke on Inflammation and Oxidative Stress: A Review. Thorax, 59, 713-721. https://doi.org/10.1136/thx.2003.012468
Biondi‐Zoccai, G., Sciarretta, S., Bullen, C., Nocella, C., Violi, F., Loffredo, L., et al. (2019) Acute Effects of Heat‐Not‐Burn, Electronic Vaping, and Traditional Tobacco Combustion Cigarettes: The Sapienza University of Rome‐Vascular Assessment of Proatherosclerotic Effects of Smoking (SUR‐VAPES) 2 Randomized Trial. JournaloftheAmericanHeartAssociation, 8, e010455. https://doi.org/10.1161/jaha.118.010455
[8]
Son, Y., Mishin, V., Laskin, J.D., Mainelis, G., Wackowski, O.A., Delnevo, C., et al. (2019) Hydroxyl Radicals in E-Cigarette Vapor and E-Vapor Oxidative Potentials under Different Vaping Patterns. ChemicalResearchinToxicology, 32, 1087-1095. https://doi.org/10.1021/acs.chemrestox.8b00400
[9]
Yogeswaran, S., Muthumalage, T. and Rahman, I. (2021) Comparative Reactive Oxygen Species (ROS) Content among Various Flavored Disposable Vape Bars, Including Cool (Iced) Flavored Bars. Toxics, 9, Article 235. https://doi.org/10.3390/toxics9100235
[10]
Münzel, T., Hahad, O., Kuntic, M., Keaney, J.F., Deanfield, J.E. and Daiber, A. (2020) Effects of Tobacco Cigarettes, E-Cigarettes, and Waterpipe Smoking on Endothelial Function and Clinical Outcomes. EuropeanHeartJournal, 41, 4057-4070. https://doi.org/10.1093/eurheartj/ehaa460
[11]
Bonner, E., Chang, Y., Christie, E., Colvin, V., Cunningham, B., Elson, D., et al. (2021) The Chemistry and Toxicology of Vaping. Pharmacology&Therapeutics, 225, Article 107837. https://doi.org/10.1016/j.pharmthera.2021.107837
[12]
Jiang, H., Ahmed, C.M.S., Martin, T.J., Canchola, A., Oswald, I.W.H., Garcia, J.A., et al. (2020) Chemical and Toxicological Characterization of Vaping Emission Products from Commonly Used Vape Juice Diluents. ChemicalResearchinToxicology, 33, 2157-2163. https://doi.org/10.1021/acs.chemrestox.0c00174
[13]
Hsu, H. and Wen, M. (2002) Lipopolysaccharide-Mediated Reactive Oxygen Species and Signal Transduction in the Regulation of Interleukin-1 Gene Expression. JournalofBiologicalChemistry, 277, 22131-22139. https://doi.org/10.1074/jbc.m111883200
[14]
Salim, S. (2014) Oxidative Stress and Psychological Disorders. CurrentNeuropharmacology, 12, 140-147. https://doi.org/10.2174/1570159x11666131120230309
[15]
Bali？, A. and Mokos, M. (2019) Do We Utilize Our Knowledge of the Skin Protective Effects of Carotenoids Enough? Antioxidants, 8, Article 259. https://doi.org/10.3390/antiox8080259
[16]
Roberts, J.L. and Moreau, R. (2016) Functional Properties of Spinach (Spinacia oleracea L.) Phytochemicals and Bioactives. Food&Function, 7, 3337-3353. https://doi.org/10.1039/c6fo00051g
[17]
Simon, A., Mazhar, S., Khokhlova, E., Leeuwendaal, N., Phipps, C., Deaton, J., et al. (2023) Solarplast？—An Enzymatically Treated Spinach Extract. Plants, 12, Article 2678. https://doi.org/10.3390/plants12142678
[18]
Rea, K., Inarejos-Garcia, A.M., Guilera Bermell, S., Garcia Bou, R., Olusoga, Y. and Deaton, J. (2024) Solarplast？ Demonstrates Anti-Inflammatory and Anti-Oxidant Activity in Vivo and Positively Modulates Perceived Anti-Ageing Quality of Life Questionnaire and Skin Analogue Scale. InternationalJournalofMolecularSciences, 25, Article 12689. https://doi.org/10.3390/ijms252312689
[19]
Bloomer, R.J., Butawan, M., van der Merwe, M. and Keating, F.H. (2020) An Assessment of the Glyconutrient Ambrotose？ on Immunity, Gut Health, and Safety in Men and Women: A Placebo-Controlled, Double-Blind, Randomized Clinical Trial. Nutrients, 12, Article 1751. https://doi.org/10.3390/nu12061751
[20]
Bielfeldt, S., Springmann, G., Seise, M., Wilhelm, K.‐P. and Callaghan, T. (2018) An Updated Review of Clinical Methods in the Assessment of Ageing Skin-New Perspectives and Evaluation for Claims Support. InternationalJournalofCosmeticScience, 40, 348-355. https://doi.org/10.1111/ics.12484
[21]
Callaghan, T.M. and Wilhelm, K.-P. (2008) A Review of Ageing and an Examination of Clinical Methods in the Assessment of Ageing Skin. Part 2: Clinical Perspectives and Clinical Methods in the Evaluation of Ageing Skin. InternationalJournalofCosmeticScience, 30, 323-332. https://doi.org/10.1111/j.1468-2494.2008.00455.x
[22]
Baradaran, A., Nasri, H. and Rafieian-Kopaei, M. (2014) Oxidative Stress and Hypertension: Possibility of Hypertension Therapy with Antioxidants. Journal of Research in Medical Sciences, 19, 358-367.
[23]
Ardalan, M.R. and Rafieian-Kopaei, M. (2013) Antioxidant Supplementation in Hypertension. Journal of Renal Injury Prevention, 3, 39-40.
[24]
Pearson, T.A., Mensah, G.A., Alexander, R.W., Anderson, J.L., Cannon, R.O., Criqui, M., et al. (2003) Markers of Inflammation and Cardiovascular Disease: Application to Clinical and Public Health Practice: A Statement for Healthcare Professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation, 107, 499-511. https://doi.org/10.1161/01.cir.0000052939.59093.45
[25]
Koh, D.-H. (2023) The Relationship between Heated Cigarette Smoking and Blood White Blood Cell Count: A Population-Based Cross-Sectional Study. PublicHealth, 222, 154-159. https://doi.org/10.1016/j.puhe.2023.07.006
[26]
Barbieri, S.S., Zacchi, E., Amadio, P., Gianellini, S., Mussoni, L., Weksler, B.B., et al. (2011) Cytokines Present in Smokers’ Serum Interact with Smoke Components to Enhance Endothelial Dysfunction. CardiovascularResearch, 90, 475-483. https://doi.org/10.1093/cvr/cvr032
[27]
Addor, F.A.S. (2017) Antioxidants in Dermatology. AnaisBrasileirosdeDermatologia, 92, 356-362. https://doi.org/10.1590/abd1806-4841.20175697
[28]
Hergesell, K., Paraskevopoulou, A., Opálka, L., Velebny, V., Vávrová, K. and Dole？ková, I. (2023) The Effect of Long-Term Cigarette Smoking on Selected Skin Barrier Proteins and Lipids. ScientificReports, 13, Article No. 11572. https://doi.org/10.1038/s41598-023-38178-7
[29]
Callaghan, T.M. and Wilhelm, K.-P. (2008) A Review of Ageing and an Examination of Clinical Methods in the Assessment of Ageing Skin. Part I: Cellular and Molecular Perspectives of Skin Ageing. InternationalJournalofCosmeticScience, 30, 313-322. https://doi.org/10.1111/j.1468-2494.2008.00454.x
[30]
Ortiz, A. and Grando, S.A. (2012) Smoking and the Skin. InternationalJournalofDermatology, 51, 250-262. https://doi.org/10.1111/j.1365-4632.2011.05205.x
[31]
Yazdanparast, T., Hassanzadeh, H., Nasrollahi, S.A., Seyedmehdi, S.M., Jamaati, H., Naimian, A., Karimi, M., Roozbahani, R. and Firooz, A. (2019) Cigarettes Smoking and Skin: A Comparison Study of the Biophysical Properties of Skin in Smokers and Non-Smokers. Tanaffos, 18, 163-168.
[32]
Wang, H., Jin, M., Xie, M., Yang, Y., Xue, F., Li, W., et al. (2023) Protective Role of Antioxidant Supplementation for Depression and Anxiety: A Meta-Analysis of Randomized Clinical Trials. JournalofAffectiveDisorders, 323, 264-279. https://doi.org/10.1016/j.jad.2022.11.072
[33]
Godos, J. and Grosso, G. (2021) Dietary Antioxidants and Brain Health: Focus on Cognitive and Affective Disorders. Antioxidants, 10, Article 1659. https://doi.org/10.3390/antiox10111659
