Background: Diabetes is a disease characterized by chronic hyperglycemia with acute and chronic complications. We have previously reported that the hydroalcoholic extract of Uvariachamae leaves and of the whole plant of Sidalinifolia exerted antihyperglycemic activity in vitro on glucose uptake using yeast cells and ex vivo on glucose absorption by Wistar rats’ muscle and intestine. Objectives: The present study aimed to evaluate the antidiabetic and antioxidant activities of Uvariachamae and Sidalinifolia extracts fractions. Methods: For fractionation, solvents of increasing polarity (n-hexane, chloroform, ethyl acetate, butanol, and distilled water) were used. Also, an evaluation of the in vitro antioxidant activity (DPPH, FRAP) and the ex vivo effect on glucose absorption by Wistar rat muscle and jejunum was performed. Results: Aqueous fractions of Uvariachamae (0.2 mg/mL) and Sidalinifolia (0.1 mg/mL) exhibited the strongest antihyperglycemic activities (P < 0.05) in rat muscle and jejunum glucose absorption tests. Phytochemistry analysis revealed the presence of phenols, flavonoids, condensed tannins, sterols, triterpenes, and reducing sugars in the fractions. Phenol, flavonoid, and tannin contents were highest in the butanol and aqueous fractions, which held the highest in vitro antioxidant activities. Conclusion: Based on the results found in ex vivo antidiabetic experiments, the fractions of Uvariachamae and Sidalinifolia can be used in further investigations as such as antidiabetic properties assessments using in vivo methods.
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