In Burundi, cassava flour is obtained through several types of preparation, including sun-drying, which gives the flour its name. The aim of this work is to determine the level of hydrocyanic acid and the nutritional value of Akambaranga. The hydrocyanic acid content was determined by the Association of Official Analytical Chemists (AOAC, 2019) and International Organization for Standardization (ISO 2164, 1975) methods, aflatoxins by the AOAC (2019) method, proteins by Kjeldahl by the AOAC (1990) method, reducing sugars and total sugars by the Luff-Schoorl reagent method and minerals by Atomic Absorption Spectrophotometry (AAS) by the AOAC (2019) method. Hydrocyanic acid was found at a level (1.35 ± 0.16 mg/kg) below Codex Alimentarius standards (<10 mg/kg), and aflatoxins were undetectable. Proteins were found in very low quantities (1.60 ± 0.57mg/kg), so Akambaranga cannot be a source of this nutrient. Total sugars and reducing sugars are in sufficient quantity. However, of the mineral salts (iron, calcium, magnesium, sodium, potassium, manganese, phosphorus, copper and zinc) analyzed, only iron (253.43 ± 148.60 mg/kg) is sufficient for daily requirements. All others need to be fortified. Proteins and minerals need to be fortified or supplemented for Akambaranga-based meals. These results will help to improve nutritional value for both consumers and manufacturers, and thus reduce malnutrition.
References
[1]
Adebayo, W.G. (2023) Cassava Production in Africa: A Panel Analysis of the Drivers and Trends. Heliyon, 9, e19939. https://doi.org/10.1016/j.heliyon.2023.e19939
[2]
Balagopalan, C., Padmaja, G., Nanda, S.K. and Moorthy, S.N. (2018) Cassava in Food, Feed and Industry. CRC Press. https://doi.org/10.1201/9781351070430
[3]
Akinpelu, A.O.L., Amamgbo, E.F., Olojede, A.O. and Oyekal, A.S. (2011) Health Implications of Cassava Production and Consumption. Journal of Agriculture and Social Research, 11, 118-125.
[4]
FAO (Food and Agriculture Organization of the United Nations) (2013) Statistical Yearbook 2013.World Food and Agriculture, Vol. 1.
[5]
Vernier, P., N’Zué, B. and Zakhia-Rozis, N. (2018) Le manioc, entre culture alimentaire et filière agro-industrielle. Quae CTA Presses agronomiques de Gembloux.
[6]
ISTEEBU (2019) Enquête Nationale sur la Situation Nutritionnelle et la Sécurité Alimentaire au Burundi (ENSNSAB), Décembre 2018. Institut de Statistiques et d’Etudes Economiques du Burundi.
[7]
Flibert, G., Abel, T. and Aly, S. (2016) African Cassava Traditional Fermented Food: The Microorganism’s Contribution to Their Nutritional and Safety Values—A Review. International Journal of Current Microbiology and Applied Sciences, 5, 664-687. https://doi.org/10.20546/ijcmas.2016.510.074
[8]
Maclntyre, R. and Graham, M. (1976) Archiv macint. International Society for Tropical Root Crops, 277 p.
[9]
Padmaja, G. and Steinkraus, K.H. (1995) Cyanide Detoxification in Cassava for Food and Feed Uses. CriticalReviewsinFoodScienceandNutrition, 35, 299-339. https://doi.org/10.1080/10408399509527703
[10]
Panghal, A., Munezero, C., Sharma, P. and Chhikara, N. (2019) Cassava Toxicity, Detoxification and Its Food Applications: A Review. ToxinReviews, 40, 1-16. https://doi.org/10.1080/15569543.2018.1560334
[11]
Aloys, N. and Hui Ming, Z. (2006) Traditional Cassava Foods in Burundi—A Review. FoodReviewsInternational, 22, 1-27. https://doi.org/10.1080/87559120500379761
[12]
INSBU (Institut National de la Statistique du Burundi) (2025) Distribution of the Population of Ordinary Households by Hill-Zone and Municipality—Preliminary Results. BUJUMBURA.
[13]
Giezendanner, F.D. (2012) Taille d’un échantillon aléatoire et Marge d’erreur. Genève. Instruction Publique, Culture et Sport, 7, 1-22
[14]
INSBU (Institut National de la Statistique du Burundi) (2024) Annuaire statistique du Burundi edition 2023.
[15]
1975.ISO: 2164: ISO 2164, 1975 (1975) International Organization for Standardiza-tion. ISO, Determination of Glycosidic Hydrocyanic Acid.
[16]
Taufik, I.I. and Guntarti, A. (2009) Comparison of Reduction Sugar Analysis Method in Cilembu Sweet Potato (Ipomoea batatas L.) Using Luff Schoorl and Anthrone Method. JurnalKedokterandanKesehatanIndonesia, 7, 219-226. https://doi.org/10.20885/jkki.vol7.iss5.art8
[17]
Degnon, G.R., Konfo, T.R.C., Adjou, S.E. and Dahouenon-Ahoussi, E. (2018) Evaluation des conditions de production, de la qualité physico-chimique et microbiologique des cossettes de manioc (Manihotesculanta Crantz) dans la commune de Bassila (Nord-Bénin). InternationalJournalofBiologicalandChemicalSciences, 12, 1528-1541. https://doi.org/10.4314/ijbcs.v12i3.36
[18]
WHO (World Health Organization) and FAO (Food and Agriculture Organization) (2016) La/les limite(s) maximale (s) pour l’acide cyanhydrique et la contamination par les mycotoxines dans le manioc et les produits à base de manioc. Codex Alimentarius CX/CF 18/12/13, 4, 1-23.
[19]
Ymdju, D., Matondo, K. and Mummguizi, B. (1995) Les moisissures toxinogènes impliquées dans le ramollissement des racines tubéreuses du manioc en fermentation sèche. Instituto Facultad de Agronomía, 367-373.
[20]
di M’balu Joachim, U., N. Thaddée, M. and Lelo, M. (2020) Inhibition du développement de l’Aspergillus flavus par l’acide acétique: Analyse de trois expériences réalisées à Kinshasa-RD Congo. JournalofAnimal&PlantSciences, 45, 7809-7821. https://doi.org/10.35759/janmplsci.v45-1.5
[21]
Oyewole, O.B. and Ayo Odunfa, S. (1989) Effects of Fermentation on the Carbohydrate, Mineral, and Protein Contents of Cassava during “Fufu” Production. JournalofFoodCompositionandAnalysis, 2, 170-176. https://doi.org/10.1016/0889-1575(89)90078-1
[22]
Chiona, M., Ntawuruhunga, P., Benesi, I., Matumba, L. and Moyo, C. (2014) Aflatoxins Contamination in Processed Cassava in Malawi and Zambia. AfricanJournalofFood, Agriculture, NutritionandDevelopment, 14, 8809-8820. https://doi.org/10.18697/ajfand.63.13080
[23]
Rosling, H. (1994) Measuring Effects in Humans of Dietary Cyanide Exposure from Cassava. ActaHorticulturae, No, 375, 271-284. https://doi.org/10.17660/actahortic.1994.375.27
[24]
Aloys, N. and Zhou, H.M. (2006) Comparative Study on Nutrient and Anti-Nutrient Changes in Ikivunde and Inyange, Two Burundian Traditionally Processed Cassava Products. JournaloftheScienceofFoodandAgriculture, 86, 1878-1886. https://doi.org/10.1002/jsfa.2547
[25]
Bradbury, J.H. (2006) Simple Wetting Method to Reduce Cyanogen Content of Cassava Flour. JournalofFoodCompositionandAnalysis, 19, 388-393. https://doi.org/10.1016/j.jfca.2005.04.012
[26]
Montagnac, J.A., Davis, C.R. and Tanumihardjo, S.A. (2009) Nutritional Value of Cassava for Use as a Staple Food and Recent Advances for Improvement. ComprehensiveReviewsinFoodScienceandFoodSafety, 8, 181-194. https://doi.org/10.1111/j.1541-4337.2009.00077.x
[27]
Milman, N.T. (2020) Dietary Iron Intake in Pregnant Women in Europe: A Review of 24 Studies from 14 Countries in the Period 1991-2014. JournalofNutritionandMetabolism, 2020, Article ID: 7102190. https://doi.org/10.1155/2020/7102190
[28]
Abrams, S.A. (2011) Dietary Guidelines for Calcium and Vitamin D: A New Era. Pediatrics, 127, 566-568. https://doi.org/10.1542/peds.2010-3576
[29]
Ekissi, A.C., Kan Kouame, B., Koko, C.A., Yao-Kouame, A. and Kati-Coulibaly, S. (2020) Détermination des minéraux des feuilles du théier de savane (Lippia multiflora). Afrique Science, 16, 139-149.
[30]
Aloys, N. and Zhou, H.M. (2006) Functional and Chemical Properties of Ikivunde and Inyange, Two Traditionally Processed Burundian Cassava Flours. JournalofFoodBiochemistry, 30, 429-443. https://doi.org/10.1111/j.1745-4514.2006.00073.x
[31]
Charles, A., Sriroth, K. and Huang, T. (2005) Proximate Composition, Mineral Contents, Hydrogen Cyanide and Phytic Acid of 5 Cassava Genotypes. FoodChemistry, 92, 615-620. https://doi.org/10.1016/j.foodchem.2004.08.024
[32]
Hawashi, M., Widjaja, T. and Gunawan, S. (2020) Solid-State Fermentation of Cassava Products for Degradation of Anti-Nutritional Value and Enrichment of Nutritional Value. In: Martínez-Espinosa, R.M., Ed., NewAdvancesonFermentationProcesses, IntechOpen. https://doi.org/10.5772/intechopen.87160
[33]
Mente, A., O’Donnell, M., Rangarajan, S., McQueen, M., Dagenais, G., Wielgosz, A., etal. (2018) Urinary Sodium Excretion, Blood Pressure, Cardiovascular Disease, and Mortality: A Community-Level Prospective Epidemiological Cohort Study. TheLancet, 392, 496-506. https://doi.org/10.1016/s0140-6736(18)31376-x
[34]
Lawal, O.M., van Stuijvenberg, L., Boon, N., Awolu, O., Fogliano, V. and Linnemann, A.R. (2021) Technological and Nutritional Properties of Amaranth‐fortified Yellow Cassava Pasta. JournalofFoodScience, 86, 5213-5225. https://doi.org/10.1111/1750-3841.15975
[35]
Berdanier, C.D. and Zempleni, J. (2009) Advanced Nutrition: Macronutrients, Micronutrients, and Metabolism. Taylor & Francis Group, LLC.
[36]
Bjørklund, G., Chartrand, M.S. and Aaseth, J. (2017) Manganese Exposure and Neurotoxic Effects in Children. EnvironmentalResearch, 155, 380-384. https://doi.org/10.1016/j.envres.2017.03.003
[37]
Cotzias, G.C. (1958) Manganese in Health and Disease. PhysiologicalReviews, 38, 503-532. https://doi.org/10.1152/physrev.1958.38.3.503
[38]
Taleb, S. and Menasria, S. (2019) Alimentation, état nutritionnel, apport calcique et pathologies associées à la ménopause chez des femmes de la ville de Tébessa (Est algérien) [Food, Nutrition, Calcium Intake, and Associated Diseases to Menopause in Women of Tebessa City (Algeria East)]. Nutrition & Santé, 8, 51-60.
[39]
Gupta, U.C. and Gupta, S.C. (2014) Sources and Deficiency Diseases of Mineral Nutrients in Human Health and Nutrition: A Review. Pedosphere, 24, 13-38. https://doi.org/10.1016/s1002-0160(13)60077-6
[40]
Bose, S., French, S., Evans, F.J., Joubert, F. and Balaban, R.S. (2003) Metabolic Network Control of Oxidative Phosphorylation. JournalofBiologicalChemistry, 278, 39155-39165. https://doi.org/10.1074/jbc.m306409200
[41]
Chan, L. and Uribarri, J. (2017) Dietary Phosphorus Intake and Kidney Function. In: Uribarri, J. and Calvo, M., Eds., Dietary Phosphorus: Health, Nutrition, and Regulatory Aspects, CRC Press, 83-91.
[42]
Takeda, E., Taketani, Y., Sawada, N., Sato, T. and Yamamoto, H. (2004) The Regulation and Function of Phosphate in the Human Body. BioFactors, 21, 345-355. https://doi.org/10.1002/biof.552210167
[43]
Pettersson, R. and Rasmussen, F. (1999) Daily Intake of Copper from Drinking Water among Young Children in Sweden. EnvironmentalHealthPerspectives, 107, 441-446. https://doi.org/10.1289/ehp.99107441
[44]
Power, M.L., Heaney, R.P., Kalkwarf, H.J., Pitkin, R.M., Repke, J.T., Tsang, R.C., etal. (1999) The Role of Calcium in Health and Disease. AmericanJournalofObstetricsandGynecology, 181, 1560-1569. https://doi.org/10.1016/s0002-9378(99)70404-7
[45]
Solomons, N.W. (1985) Biochemical, Metabolic, and Clinical Role of Copper in Human Nutrition. JournaloftheAmericanCollegeofNutrition, 4, 83-105. https://doi.org/10.1080/07315724.1985.10720069
[46]
Bretti, C., Cigala, R.M., Lando, G., Milea, D. and Sammartano, S. (2012) Sequestering Ability of Phytate toward Biologically and Environmentally Relevant Trivalent Metal Cations. JournalofAgriculturalandFoodChemistry, 60, 8075-8082. https://doi.org/10.1021/jf302007v
[47]
Johanita, K., Annelies, B., Luc, P. and Nina, H. (2018) Vegetable Relishes, High in β-Carotene, Increase the Iron, Zinc and β-Carotene Nutritive Values from Cereal Porridges. International Journal of Food Sciences and Nutrition, 69, 291-297.
[48]
Essack, H., Odhav, B. and Mellem, J.J. (2017) Screening of Traditional South African Leafy Vegetables for Specific Anti-Nutritional Factors before and after Processing. FoodScienceandTechnology, 37, 462-471. https://doi.org/10.1590/1678-457x.20416
[49]
Mongwaketse, T., Kruger, J., Lewies, A., Baumgartner, J. and Mattheus Smuts, C. (2022) Minerals, Antinutrients Content and the Bioaccessibility of Iron and Zinc in Cooked, Spontaneously Fermented-Dried, and Blanched-Dried Dark Green Leafy Vegetables Commonly Consumed in Sub-Saharan Africa. FoodScienceandTechnology, 42, e37921. https://doi.org/10.1590/fst.37921
[50]
Tripathi, M.K. and Mishra, A.S. (2007) Glucosinolates in animal nutrition: A review. AnimalFeedScienceandTechnology, 132, 1-27. https://doi.org/10.1016/j.anifeedsci.2006.03.003
[51]
Stephens, T.V., Payne, M., Ball, R.O., Pencharz, P.B. and Elango, R. (2015) Protein Requirements of Healthy Pregnant Women during Early and Late Gestation Are Higher than Current Recommendations. TheJournalofNutrition, 145, 73-78. https://doi.org/10.3945/jn.114.198622
[52]
Cannon, P.R., Wissler, R.W., Woolridge, R.L. and Bendtt, E.P. (1948) The Relationship of Protein Deficiency to Infection. Nutrition Reviews, 3, 78-79. https://doi.org/10.1111/j.1753-4887.1945.tb08552.x
[53]
Vis, H.L. (1969) Protein Deficiency Disorders. PostgraduateMedicalJournal, 45, 107-115. https://doi.org/10.1136/pgmj.45.520.107
[54]
Slattery, R.A., Walker, B.J., Weber, A.P.M. and Ort, D.R. (2017) The Impacts of Fluctuating Light on Crop Performance. PlantPhysiology, 176, 990-1003. https://doi.org/10.1104/pp.17.01234
[55]
Wang, Y. (2024) Improving Photosynthetic Efficiency in Fluctuating Light to Enhance Yield of C3 and C4 Crops. CropandEnvironment, 3, 184-193. https://doi.org/10.1016/j.crope.2024.06.003
[56]
Gómez, G., Valdivieso, M. and Noma, A.T. (1985) The Influence of Cultivar and Plant Age on the Chemical Composition of Field-Grown Cassava Leaves and Roots. QualitasPlantarumPlantFoodsforHumanNutrition, 35, 109-119. https://doi.org/10.1007/bf01092126
[57]
Zvauya, R. and Muzondo, M.I. (1995) Reduction of Cyanide Levels in Cassava during Sequential Sundrying and Solid State Fermentation. InternationalJournalofFoodSciencesandNutrition, 46, 13-16. https://doi.org/10.3109/09637489509003380
[58]
Visvanathan, R., Houghton, M.J. and Williamson, G. (2021) Maltoheptaoside Hydrolysis with Chromatographic Detection and Starch Hydrolysis with Reducing Sugar Analysis: Comparison of Assays Allows Assessment of the Roles of Direct α-Amylase Inhibition and Starch Complexation. FoodChemistry, 343, Article ID: 128423. https://doi.org/10.1016/j.foodchem.2020.128423
[59]
Levine, H. and Slade, L. (1986) A Polymer Physico-Chemical Approach to the Study of Commercial Starch Hydrolysis Products (SHPs). CarbohydratePolymers, 6, 213-244. https://doi.org/10.1016/0144-8617(86)90021-4