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草乌化学成分分离及对党参虫害杀虫研究
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Abstract:
研究草乌化学成分以及对党参主要虫害的杀虫研究。方法：采用正相硅胶柱柱层析、反相硅胶柱层析、大孔树脂柱层析等方法进行化学成分的分离鉴定；用微量点滴法以及叶片浸渍法进行触杀以及胃毒活性研究。结果：分离鉴定出四种二萜生物碱，其中新乌头碱以及苯甲酰乌头原碱表现出较强的触杀活性，LC50值为0.797 mg/mL和0.676 mg/mL。
The study focused on the chemical constituents of Aconitum carmichaelii and their insecticidal activities against the major pests of Codonopsis pilosula. Methods: Chemical constituents were isolated and identified by normal phase silica gel column chromatography, reverse phase silica gel column chromatography, and macroporous resin column chromatography. Contact and stomach toxicity were investigated using the micro-droplet method and leaf immersion method. Results: Four diterpene alkaloids were isolated and identified. Among them, mesaconitine and benzoylaconitine showed strong contact toxicity with LC50 values of 0.797 mg/mL and 0.676 mg/mL, respectively.
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