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茶皂素对真菌液体发酵产纤维素酶的影响及酶学特性研究
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Abstract:
本实验对厚垣镰孢霉HML278采用液体发酵产纤维素酶，加入0.2%、0.4%、0.6%、0.8%、1.0%不同浓度的茶皂素，探究茶皂素对其产纤维素酶的影响。实验结果显示，加入0.6%浓度的茶皂素的纤维素酶活性最高。在酶学特性研究中，酶的最适温度为60℃，最适pH为6.4。在金属离子对酶活性的探究中，发现Li+、K+、Ca2+、Mn2+、Zn2+、Fe3+对酶促反应具有促进作用，其中Ca2+、Mn2+对酶促反应的促进作用最强；而Co2+、Al3+对酶促反应具有抑制作用。
This experiment used liquid fermentation to produce cellulase from Fusarium graminearum HML278, and added tea saponin at different concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% to investigate the effect of tea saponin on its cellulase production. The experimental results showed that the cellulase activity was highest when 0.6% concentration of tea saponin was added. In the study of enzymatic characteristics, the optimal temperature and pH for the enzyme are 60？C and 6.4, respectively. In the exploration of metal ions on enzyme activity, it was found that Li+, K+, Ca2+, Mn2+, Zn2+, and Fe3+ have a promoting effect on enzymatic reactions, among which Ca2+ and Mn2+ have the strongest promoting effect on enzymatic reactions; Co2+ and Al3+ have inhibitory effects on enzymatic reactions.
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