%0 Journal Article %T 茶皂素对真菌液体发酵产纤维素酶的影响及酶学特性研究
Study on the Effect of Tea Saponin on Fungal Liquid Fermentation to Produce Cellulase and Its Enzymatic Characteristics %A 张健 %J Hans Journal of Agricultural Sciences %P 616-625 %@ 2164-5523 %D 2025 %I Hans Publishing %R 10.12677/hjas.2025.155076 %X 本实验对厚垣镰孢霉HML278采用液体发酵产纤维素酶，加入0.2%、0.4%、0.6%、0.8%、1.0%不同浓度的茶皂素，探究茶皂素对其产纤维素酶的影响。实验结果显示，加入0.6%浓度的茶皂素的纤维素酶活性最高。在酶学特性研究中，酶的最适温度为60℃，最适pH为6.4。在金属离子对酶活性的探究中，发现Li⁺、K⁺、Ca²⁺、Mn²⁺、Zn²⁺、Fe³⁺对酶促反应具有促进作用，其中Ca²⁺、Mn²⁺对酶促反应的促进作用最强；而Co²⁺、Al³⁺对酶促反应具有抑制作用。
This experiment used liquid fermentation to produce cellulase from Fusarium graminearum HML278, and added tea saponin at different concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% to investigate the effect of tea saponin on its cellulase production. The experimental results showed that the cellulase activity was highest when 0.6% concentration of tea saponin was added. In the study of enzymatic characteristics, the optimal temperature and pH for the enzyme are 60˚C and 6.4, respectively. In the exploration of metal ions on enzyme activity, it was found that Li⁺, K⁺, Ca²⁺, Mn²⁺, Zn²⁺, and Fe³⁺ have a promoting effect on enzymatic reactions, among which Ca²⁺ and Mn²⁺ have the strongest promoting effect on enzymatic reactions; Co²⁺ and Al³⁺ have inhibitory effects on enzymatic reactions. %K 茶皂素， %K 厚垣镰孢霉， %K 酶学性质， %K 酶活， %K 纤维素酶
Tea Saponin %K Fusarium oxysporum %K Enzymatic Characteristics %K Enzyme Activity %K Cellulase %U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=115159