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微量紫外–可见分光光度法测定阳离子脂质体中MicroRNA的含量
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Abstract:
目的：建立以硬质胺制备载MicroRNA阳离子脂质体中RNA含量测定的方法。方法：采用Q5000微量紫外–可见分光光度计，检测波长为260 nm，进行标准曲线绘制，精密度，重复性，稳定性及回收率等考察，建立可靠的、灵敏的RNA含量测定方法。结果：得到MicroRNA的线性回归方程：Y = 1.0378X ？ 4.054 6，(R2 = 0.9984)，在3.32~1091.22 ng/μL范围内具有良好的线性关系，精密度RSD值为0.41%，重复性RSD值为3.62%，稳定性RSD值为0.63%，加样回收率平均值为99.80%，RSD值为1.81%。阳离子脂质体中MicroRNA含量为200.73 ± 10.31 ng/μL。结论：本实验方法简便准确、重复性好、灵敏度高、含量测定方法稳定可行，适用于阳离子脂质体中MicroRNA的含量测定。
Objective: To establish a method for the determination of RNA in MicroRNA cationic liposomes prepared with Stearic amine. Methods: A Q5000 UV-visible spectrophotometer was used with the detection wavelength of 260 nm, and the standard curve drawing, precision, repeatability, stability and recovery were investigated to establish a reliable and sensitive method for the determination of RNA content. Results: The linear regression equation for MicroRNA was obtained: Y = 1.0378X ？ 4.0546, (R2 = 0.9984), with good linearity in the range of 3.32~1091.22 ng/μL, precision RSD value of 0.41%, repeatability RSD value of 3.62%, stability RSD value of 0.63%, mean spiked recovery of 99.80%, and the RSD value was 1.81%. The content of MicroRNA in cationic liposomes was 200.73 ± 10.31 ng/μL. Conclusion: The established present experimental method was simple, accurate, reproducible, sensitive, stable and could be used for the determination of MicroRNA in cationic liposomes.
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