Inhibitory Activity of Methanolic Extracts of Six (06) Medicinal Plants on the Growth of Escherichia coli Resistant to Imipenem by Production of Carbapenemase Isolated at the Tengandogo Hospital in Ouagadougou
The fight against bacterial infections is essentially based on the use of antibiotics. Unfortunately, bacteria have developed resistance mechanisms against antibiotics, particularly those of the β-lactam family. Thus, this study aims to evaluate the inhibitory activity of six medicinal plants resulting from an ethnobotanical investigation of these formidable bacteria. Plant methanol extracts were obtained using Soxhlet. The photometric method was used to measure the absorbances of bacterial (Escherichia coli) suspensions and for the phytochemical assay of plant extracts. Terminalia laxiflora (T. laxiflora) Engl. & Diels proves to be the most effective plant with an optical density (OD) of 0.322 against 1.133 of the positive control and 0.901 of the antibiotic. The total phenolic content of the methanolic extract of T. laxiflora Engl. & Diels was 68.45 ± 0.11 mg EAG/100 mg extract. The total flavonoids were 2.71 ± 0.09 mg EQ/100mg of extract. The antioxidant content of the extract (820.46 ± 0.17 μmol EAA/g of extract) is higher than the contents of quercetin (646.85 μmol EAA/g of extract) and trolox (765.99 μmol EAA/g of extract). As for the FRAP method, the extract gave a content (4190.93 ± 1.79 μmol EAA/g of extract) which exceeds that of quercetin (5991.29 ± 1.33 μmol EAA/g of extract). At the level of the ABTS method, the extract still gave a value (11935.45 ±3.18 μmol EAA/g of extract) higher than trolox (8137.61 ± 4.04 μmol EAA/g of extract).
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