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Pharmacy Information 2025
罗布麻蛋白多肽部位制备及其抗衰老作用研究
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Abstract:
目的：探讨罗布麻蛋白多肽部位对过氧化氢(H2O2)、D-半乳糖诱导的人脐静脉内皮细胞(Human Umbilical Vein Endothelial Cells, HUVEC)衰老的作用。方法：采用碱提酸沉法提取罗布麻蛋白(LQ)，采用胃–胰蛋白酶进行酶解得到罗布麻蛋白多肽部位(LH)。以过氧化氢(H2O2)、D-半乳糖诱导的人脐静脉内皮细胞作为衰老模型，通过对细胞活力、β-半乳糖苷酶活性、活性氧(ROS)含量和线粒体膜电位指标进行测定，研究罗布麻蛋白及多肽对HUVEC衰老细胞的缓解作用。结果：与模型组相比，给予罗布麻蛋白多肽可显著增加HUVEC衰老细胞的细胞活力和线粒体膜电位(P < 0.05)，显著降低β-半乳糖苷酶阳性细胞比例和ROS荧光强度(P < 0.05)。结论：罗布麻蛋白及多肽部位可抑制由衰老诱发的氧化应激损伤，从而延缓衰老。
Objective: To investigate the effect of the protein polypeptide site of Apocynum venetum L., which is on hydrogen peroxide (H2O2) and D-galactose-induced senescence of human umbilical vein endothelial cells (HUVECs). Methods: The polypeptide part (LH) of Apocynum venetum L. protein (LQ) was extracted by alkali extraction and acid precipitation method, and Apocynum venetum L. protein polypeptide site (LH) was obtained by enzymatic hydrolysis by gastric-trypsin. Hydrogen peroxide (H2O2) and D-galactose-induced human umbilical vein endothelial cells were used as a model of senescence, and the alleviating effects of Apocynum venetum L. protein and polypeptides on HUVEC senescent cells were studied by measuring cell viability, β-galactosidase activity, reactive oxygen species (ROS) content and mitochondrial membrane potential. Results: Compared with the model group, the administration of Apocynum venetum L. protein polypeptide significantly increased the cell viability and mitochondrial membrane potential of HUVEC senescent cells (P < 0.05), and significantly decreased the proportion of β-galactosidase-positive cells and ROS fluorescence intensity (P < 0.05). Conclusion: Apocynum venetum L. protein and polypeptide sites can inhibit oxidative stress damage induced by aging, thereby delaying aging.
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