%0 Journal Article
%T 罗布麻蛋白多肽部位制备及其抗衰老作用研究<br>Study on Preparation of <i>Apocynum</i> <i>venetum</i><i> L.</i> Protein Polypeptide Site and Its Anti-Aging Effect
%A 谈慧
%A 刘吉华
%J Pharmacy Information
%P 162-171
%@ 2160-4452
%D 2025
%I Hans Publishing
%R 10.12677/pi.2025.143020
%X 目的&#65306;探讨罗布麻蛋白多肽部位对过氧化氢(H<sub>2</sub>O<sub>2</sub>)、D-半乳糖诱导的人脐静脉内皮细胞(Human Umbilical Vein Endothelial Cells, HUVEC)衰老的作用。方法&#65306;采用碱提酸沉法提取罗布麻蛋白(LQ)&#65292;采用胃&#8211;胰蛋白酶进行酶解得到罗布麻蛋白多肽部位(LH)。以过氧化氢(H<sub>2</sub>O<sub>2</sub>)、D-半乳糖诱导的人脐静脉内皮细胞作为衰老模型&#65292;通过对细胞活力、<i>&#946;</i>-半乳糖苷酶活性、活性氧(ROS)含量和线粒体膜电位指标进行测定&#65292;研究罗布麻蛋白及多肽对HUVEC衰老细胞的缓解作用。结果&#65306;与模型组相比&#65292;给予罗布麻蛋白多肽可显著增加HUVEC衰老细胞的细胞活力和线粒体膜电位(<i>P</i> &lt; 0.05)&#65292;显著降低<i>&#946;</i>-半乳糖苷酶阳性细胞比例和ROS荧光强度(<i>P</i> &lt; 0.05)。结论&#65306;罗布麻蛋白及多肽部位可抑制由衰老诱发的氧化应激损伤&#65292;从而延缓衰老。<br>Objective: To investigate the effect of the protein polypeptide site of <i>Apo</i><i>cynum</i> <i>venetum</i><i> L.</i>, which is on hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and D-galactose-induced senescence of human umbilical vein endothelial cells (HUVECs). Methods: The polypeptide part (LH) of <i>Apocynum</i> <i>venetum</i><i> L.</i> protein (LQ) was extracted by alkali extraction and acid precipitation method, and <i>Apocynum</i> <i>venetum</i><i> L.</i> protein polypeptide site (LH) was obtained by enzymatic hydrolysis by gastric-trypsin. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and D-galactose-induced human umbilical vein endothelial cells were used as a model of senescence, and the alleviating effects of <i>Apocynum</i> <i>venetum</i><i> L.</i> protein and polypeptides on HUVEC senescent cells were studied by measuring cell viability, <i>&#946;</i>-galactosidase activity, reactive oxygen species (ROS) content and mitochondrial membrane potential. Results: Compared with the model group, the administration of <i>Apocynum</i> <i>venetum</i><i> L.</i> protein polypeptide significantly increased the cell viability and mitochondrial membrane potential of HUVEC senescent cells (<i>P</i> &lt; 0.05), and significantly decreased the proportion of <i>&#946;</i>-galactosidase-positive cells and ROS fluorescence intensity (<i>P</i> &lt; 0.05). Conclusion: <i>Apocynum</i> <i>venetum</i><i> L.</i> protein and polypeptide sites can inhibit oxidative stress damage induced by aging, thereby delaying aging.
%K 罗布麻&#65292
%K 抗衰老&#65292
%K 蛋白多肽部位<br><i>Apocynum</i> <i>venetum</i><i> L</i><i>.</i><i>
%K </i> Anti-Aging
%K Protein Polypeptide Site
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=113820