Objective: To establish a low-cost way to evaluate the efficacy of hypoglycemic herbs. Methods: The silkworms were divided into control group, model group, metformin group, and various herbal extracts group, and were reared at 16？C - 20？C until the sixth day of modeling, and blood glucose was detected in all silkworms on the sixth day. Results: High-jasmine crude polysaccharide (H-JCP), total flavonoids of Paederia scandens (TFPS), total flavonoids of hibiscus flowers (TFHF), low-total flavonoids of chamomile (L-TFC), high-total flavonoids of chamomile (H-TFC), low-total flavonoids of potentilla discolor (L-TFPD), high-total flavonoids of potentilla discolor (H-TFPD), low-golden flowers tea extract (L-GFTE), high-golden flowers tea extract (H-GFTE), low-guava extract (L-GE) and high-guava extract(H-GE) had different hypoglycemic effects in the silkworm, respectively, H-JCP, TFPS, TFHF, H-TFC had a certain inhibitory effect on α-glucosidase activity, while crude polysaccharide of Polyporus umbellatus (CPPU), buckwheat shell total flavonoids (BSTF), low-hawthorn leaves flavonoids (L-HLF) and high-hawthorn leaves flavonoids (H-HLF) were not as effective in lowering the hypoglycemic effect. Conclusion: A model of diabetes in the silkworm, established at low temperatures, allowed a preliminary evaluation of the hypoglycemic effect of the herbal extracts.
References
[1]
Ong, K.L., Stafford, L.K., McLaughlin, S.A., et al. (2023) Global, Regional, and National Burden of Diabetes from 1990 to 2021, with Projections of Prevalence to 2050: a Systematic Analysis for the Global Burden of Disease Study 2021. The Lancet, 402, 203-234.
[2]
Pandey, S., Chmelir, T. and Chottova Dvorakova, M. (2023) Animal Models in Diabetic Research—History, Presence, and Future Perspectives. Biomedicines, 11, Article 2852. https://doi.org/10.3390/biomedicines11102852
[3]
Lutz, T.A. (2023) Mammalian Models of Diabetes Mellitus, with a Focus on Type 2 Diabetes Mellitus. Nature Reviews Endocrinology, 19, 350-360. https://doi.org/10.1038/s41574-023-00818-3
[4]
Hou, J., Tan, C., Chen, N., Zhou, Y., Huang, S., Chen, H., et al. (2024) Establishment of Diabetes Mellitus Model Using Bombyx mori Silkworms in a Low‐Temperature Environment. Archives of Insect Biochemistry and Physiology, 115, e22083. https://doi.org/10.1002/arch.22083
[5]
Aznar-Cervantes, S.D., Monteagudo Santesteban, B. and Cenis, J.L. (2021) Products of Sericulture and Their Hypoglycemic Action Evaluated by Using the Silkworm, Bombyx mori (Lepidoptera: Bombycidae), as a Model. Insects, 12, Article 1059. https://doi.org/10.3390/insects12121059
[6]
Nwibo, D.D., Hamamoto, H., Matsumoto, Y., Kaito, C. and Sekimizu, K. (2015) Current Use of Silkworm Larvae (Bombyx mori) as an Animal Model in Pharmaco-Medical Research. Drug Discoveries & Therapeutics, 9, 133-135. https://doi.org/10.5582/ddt.2015.01026
[7]
Chen, J., Mangelinckx, S., Adams, A., Wang, Z., Li, W. and De Kimpe, N. (2015) Natural Flavonoids as Potential Herbal Medication for the Treatment of Diabetes Mellitus and Its Complications. Natural Product Communications, 10, 187-200. https://doi.org/10.1177/1934578x1501000140
[8]
Wu, J., Shi, S., Wang, H. and Wang, S. (2016) Mechanisms Underlying the Effect of Polysaccharides in the Treatment of Type 2 Diabetes: A Review. Carbohydrate Polymers, 144, 474-494. https://doi.org/10.1016/j.carbpol.2016.02.040
[9]
Lyu, H., Chen, J. and Li, W. (2016) Natural Triterpenoids for the Treatment of Diabetes Mellitus: A Review. Natural Product Communications, 11, 1579-1586. https://doi.org/10.1177/1934578x1601101037
[10]
Xi, M., Hai, C., Tang, H., Chen, M., Fang, K. and Liang, X. (2008) Antioxidant and Antiglycation Properties of Total Saponins Extracted from Traditional Chinese Medicine Used to Treat Diabetes Mellitus. Phytotherapy Research, 22, 228-237. https://doi.org/10.1002/ptr.2297
[11]
Tan, K., Tesar, C., Wilton, R., Jedrzejczak, R.P. and Joachimiak, A. (2018) Interaction of Antidiabetic α‐Glucosidase Inhibitors and Gut Bacteria α‐Glucosidase. Protein Science, 27, 1498-1508. https://doi.org/10.1002/pro.3444
[12]
Liu, S., Li, D., Huang, B., Chen, Y., Lu, X. and Wang, Y. (2013) Inhibition of Pancreatic Lipase, α-Glucosidase, α-Amylase, and Hypolipidemic Effects of the Total Flavonoids from Nelumbo nucifera Leaves. Journal of Ethnopharmacology, 149, 263-269. https://doi.org/10.1016/j.jep.2013.06.034
[13]
Zhang, X., Xue, R., Cao, G., Pan, Z., Zheng, X. and Gong, C. (2012) Silkworms Can Be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs. Journal of Insect Science, 12, Article 4. https://doi.org/10.1673/031.012.0401
[14]
Hamamoto, H., Horie, R. and Sekimizu, K. (2019) Pharmacokinetics of Anti-Infectious Reagents in Silkworms. Scientific Reports, 9, Article No. 9451. https://doi.org/10.1038/s41598-019-46013-1
[15]
Ishii, M., Matsumoto, Y., Yamada, T., Abe, S. and Sekimizu, K. (2017) An Invertebrate Infection Model for Evaluating Anti-Fungal Agents against Dermatophytosis. Scientific Reports, 7, Article No. 12289. https://doi.org/10.1038/s41598-017-12523-z
[16]
Matsumoto, Y. (2020) Facilitating Drug Discovery in Human Disease Models Using Insects. Biological and Pharmaceutical Bulletin, 43, 216-220. https://doi.org/10.1248/bpb.b19-00834
[17]
Shukla, E., Thorat, L.J., Nath, B.B. and Gaikwad, S.M. (2015) Insect Trehalase: Physiological Significance and Potential Applications. Glycobiology, 25, 357-367. https://doi.org/10.1093/glycob/cwu125
[18]
Matsumoto, Y., Ishii, M., Hayashi, Y., Miyazaki, S., Sugita, T., Sumiya, E., et al. (2015) Diabetic Silkworms for Evaluation of Therapeutically Effective Drugs against Type II Diabetes. Scientific Reports, 5, Article No. 10722. https://doi.org/10.1038/srep10722
[19]
Iwami, M., Kawakami, A., Ishizaki, H., Takahashi, S.Y., Adachi, T., Suzuki, Y., et al. (1989) Cloning of a Gene Encoding Bombyxin, an Insulin‐Like Brain Secretory Peptide of the Silkmoth Bombyx mori with Prothoracicotropic Activity. Development, Growth & Differentiation, 31, 31-37. https://doi.org/10.1111/j.1440-169x.1989.00031.x
[20]
Zhang, Z., Zhang, S., Niu, B., Ji, D., Liu, X., Li, M., et al. (2019) A Determining Factor for Insect Feeding Preference in the Silkworm, Bombyx mori. PLOS Biology, 17, e3000162. https://doi.org/10.1371/journal.pbio.3000162
[21]
Nasri, H., Rafieian-Kopaei, M. (2014) Metformin: Current Knowledge. Journal of Research in Medical Sciences, 19, 658-664.
[22]
Lu, H., Xie, T., Wu, Q., Hu, Z., Luo, Y. and Luo, F. (2023) Alpha-Glucosidase Inhibitory Peptides: Sources, Preparations, Identifications, and Action Mechanisms. Nutrients, 15, Article 4267. https://doi.org/10.3390/nu15194267
[23]
Kalra, S. (2014) Incretin Enhancement without Hyperinsulinemia: α-Glucosidase Inhibitors. Expert Review of Endocrinology & Metabolism, 9, 423-425. https://doi.org/10.1586/17446651.2014.931807
[24]
Kashtoh, H. and Baek, K. (2022) Recent Updates on Phytoconstituent Alpha-Glucosidase Inhibitors: An Approach towards the Treatment of Type Two Diabetes. Plants, 11, Article 2722. https://doi.org/10.3390/plants11202722
[25]
Han, H., Kang, G., Kim, J.S., Choi, B.H. and Koo, S. (2016) Regulation of Glucose Metabolism from a Liver-Centric Perspective. Experimental & Molecular Medicine, 48, e218. https://doi.org/10.1038/emm.2015.122
