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聚氨酯三维肺癌模型的建立及姜黄素药敏试验
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Abstract:
目的：通过聚氨酯构建三维(3D)肺癌模型，并用于姜黄素药敏试验。方法：聚氨酯泡沫支架经I型胶原表面修饰后种植A549细胞构建肺癌3D模型，并进行姜黄素药敏试验。扫描电镜观察肺癌A549细胞生长情况。并均与2D培养下进行比较。结果：姜黄素可抑制A549的生长，浓度提高，抑制加强，呈剂量依赖性。3D培养条件下的细胞生长抑制率显著小于2D培养。结论：与2D培养相比，聚氨酯3D肺癌模型可能更好地模拟体内肺癌肿瘤微环境，提高临床前药物研究的预测能力，改善药物临床转化。
Objective: To construct a three-dimensional (3D) lung cancer model by polyurethane and use it for curcumin drug sensitivity test. Methods: The 3D model of lung cancer was constructed by transplantation of A549 cells into polyurethane foam scaffolded with collagen type I surface modification, and curcumin susceptibility test was performed. The growth of lung cancer A549 cells was observed by scanning electron microscope. Then the results were compared with 2D culture. Results: Curcumin could inhibit the growth of A549. The concentration increased and the inhibition increased in a dose-dependent manner. The inhibition rate of 3D culture was significantly lower than that of 2D culture. Conclusion: Compared with 2D culture, polyurethane 3D lung cancer model may better simulate the microenvironment of lung cancer in vivo, and improve the predictive ability of preclinical drug studies, and improve the clinical transformation of drugs.
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